Hox gene expression in the embryonic genital system of the sea turtle Lepidochelys olivacea (Eschscholt, 1829), a species with temperature-dependent sex determination

2010 ◽  
Vol 10 (6) ◽  
pp. 290-298 ◽  
Author(s):  
Itzel Sifuentes-Romero ◽  
Horacio Merchant-Larios ◽  
Alejandra García-Gasca
Keyword(s):  
Gene Expression ◽  
Sex Determination ◽  
Sea Turtle ◽  
Genital System ◽  
Temperature Dependent ◽  
Hox Gene ◽  
Lepidochelys Olivacea

Spatiotemporal Expression of Foxl2 and Dmrt1 before, during, and after Sex Determination in the Sea Turtle Lepidochelys olivacea

2019 ◽  
Vol 13 (5-6) ◽  
pp. 286-296
Author(s):  
Verónica Díaz-Hernández ◽  
Paloma Dominguez-Mora ◽  
Luis Chino-Palomo ◽  
Alejandro Marmolejo-Valencia ◽  
Martha Harfush ◽  
...  
Keyword(s):  
Sex Determination ◽  
Sea Turtle ◽  
Sexually Dimorphic ◽  
Cell Nuclei ◽  
Temperature Dependent ◽  
Olive Ridley ◽  
Lepidochelys Olivacea ◽  
Spatiotemporal Expression ◽  
Dimorphic Expression ◽  
Medullary Cords

The sex of sea turtles is determined by temperature during egg incubation. Thus, climate change affects the sex ratio, exacerbating their vulnerability to extinction. Understanding spatiotemporal effects of temperature on sex determination at the gonadal level may facilitate the design of strategies to mitigate the effects of global warming. Here, we used qRT-PCR and immunofluorescence to analyze the spatiotemporal expression of <i>Dmrt1 </i>and <i>Foxl2</i> in developing gonads of <i>Lepidochelys olivacea</i> incubated at male-producing temperature (MPT, 26°C) or female-producing temperature (FPT, 33°C). Although both transcription factors are expressed in bipotential gonads up to stage 25, the timing of their sexually dimorphic regulation differs. Whereas the dimorphic expression of Dmrt1 protein initiates at stage 24, Foxl2 protein was expressed specifically in females at stage 25. Interestingly, whereas Dmrt1 colocalizes with Sox9 in cell nuclei of primary medullary cords to form the testis cords, Foxl2 protein is first detected in Sox9-negative cells of primary medullary cords, prior to its substantial expression in the ovarian cortex. Thus, results suggest that the temperature-dependent regulation of sexual pathways is stochastic among the cells of primary medullary cords in undifferentiated bipotential gonads of the olive ridley.

Dimorphic DNA methylation during temperature-dependent sex determination in the sea turtle Lepidochelys olivacea

2016 ◽  
Vol 236 ◽  
pp. 35-41 ◽  
Author(s):  
Daniela Venegas ◽  
Alejandro Marmolejo-Valencia ◽  
Christian Valdes-Quezada ◽  
Tzipe Govenzensky ◽  
Félix Recillas-Targa ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Sex Determination ◽  
Sea Turtle ◽  
Temperature Dependent ◽  
Lepidochelys Olivacea

Expression profiles of Dax1, Dmrt1, and Sox9 during temperature sex determination in gonads of the sea turtle Lepidochelys olivacea

2002 ◽  
Vol 129 (1) ◽  
pp. 20-26 ◽  
Author(s):  
L.C Torres Maldonado ◽  
A Landa Piedra ◽  
N Moreno Mendoza ◽  
A Marmolejo Valencia ◽  
A Meza Martı́nez ◽  
...  
Keyword(s):  
Sex Determination ◽  
Expression Profiles ◽  
Sea Turtle ◽  
Lepidochelys Olivacea

Gonadal Morphogenesis and Gene Expression in Reptiles with Temperature-Dependent Sex Determination

2010 ◽  
Vol 4 (1-2) ◽  
pp. 50-61 ◽  
Author(s):  
H. Merchant-Larios ◽  
V. Díaz-Hernández ◽  
A. Marmolejo-Valencia
Keyword(s):  
Gene Expression ◽  
Sex Determination ◽  
Temperature Dependent

scholarly journals Temperature-dependent effects of house fly proto-Y chromosomes on gene expression act independently of the sex determination pathway

2020 ◽  
Author(s):  
Kiran Adhikari ◽  
Jae Hak Son ◽  
Anna H. Rensink ◽  
Jaweria Jaweria ◽  
Daniel Bopp ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sex Determination ◽  
Sex Chromosomes ◽  
Developmental Process ◽  
Natural Populations ◽  
House Fly ◽  
Sexually Dimorphic ◽  
Temperature Dependent ◽  
Y Chromosomes ◽  
Polygenic Sex Determination

AbstractSex determination, the developmental process by which sexually dimorphic phenotypes are established, evolves fast. Species with polygenic sex determination, in which master regulatory genes are found on multiple different proto-sex chromosomes, are informative models to study the evolution of sex determination. House flies are such a model system, with male determining loci possible on all six chromosomes and a female-determiner on one of the chromosomes as well. The distributions of the two most common male-determining proto-Y chromosomes across natural populations suggests that temperature variation is an important selection pressure responsible for maintaining polygenic sex determination in this species. To test that hypothesis, we used RNA-seq to identify temperature-dependent effects of the proto-Y chromosomes on gene expression. We find no evidence for ecologically meaningful temperature-dependent expression of sex determining genes between male genotypes, but we identified hundreds of other genes whose expression depends on the interaction between proto-Y chromosome genotype and temperature. Notably, genes with genotype-by-temperature interactions on expression are not enriched on the proto-sex chromosomes. Moreover, there is no evidence that temperature-dependent expression is driven by chromosome-wide expression divergence between the proto-Y and proto-X alleles. Therefore, if temperature-dependent gene expression is responsible for differences in phenotypes and fitness of proto-Y genotypes across house fly populations, these effects are driven by a small number of temperature-dependent alleles on the proto-Y chromosomes.

Temperature-Controlled Sex Determination in the Sea Turtle Lepidochelys olivacea

1983 ◽  
Vol 17 (4) ◽  
pp. 404 ◽  
Author(s):  
C. J. McCoy ◽  
Richard C. Vogt ◽  
Ellen J. Censky
Keyword(s):  
Sex Determination ◽  
Sea Turtle ◽  
Lepidochelys Olivacea ◽  
Temperature Controlled
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