scholarly journals Modulation of dimethylsulfoniopropionate (DMSP) concentration in an Alexandrium minutum (Dinophyceae) culture by small-scale turbulence: A link to toxin production?

Harmful Algae ◽  
2011 ◽  
Vol 11 ◽  
pp. 88-95 ◽  
Author(s):  
Elisa Berdalet ◽  
Gisela Llaveria ◽  
Rafel Simó
2007 ◽  
Vol 4 (4) ◽  
pp. 559-567 ◽  
Author(s):  
L. Bolli ◽  
G. Llaveria ◽  
E. Garcés ◽  
Ò. Guadayol ◽  
K. van Lenning ◽  
...  

Abstract. Some dinoflagellate species have shown different physiological responses to certain turbulent conditions. Here we investigate how two levels of turbulent kinetic energy dissipation rates (ε = 0.4 and 27 cm² s−3) affect the PSP toxins and ecdysal cyst dynamics of two bloom forming species, Alexandrium minutum and A. catenella. The most striking responses were observed at the high ε generated by an orbital shaker. In the cultures of the two species shaken for more than 4 days, the cellular GTX(1+4) toxin contents were significantly lower than in the still control cultures. In A. minutum this trend was also observed in the C(1+2) toxin content. For the two species, inhibition of ecdysal cyst production occurred during the period of exposure of the cultures to stirring (4 or more days) at any time during their growth curve. Recovery of cyst abundances was always observed when turbulence stopped. When shaking persisted for more than 4 days, the net growth rate significantly decreased in A. minutum (from 0.25±0.01 day−1 to 0.19±0.02 day−1) and the final cell numbers were lower (ca. 55.4%) than in the still control cultures. In A. catenella, the net growth rate was not markedly modified by turbulence although under long exposure to shaking, the cultures entered earlier in the stationary phase and the final cell numbers were significantly lower (ca. 23%) than in the control flasks. The described responses were not observed in the experiments performed at the low turbulence intensities with an orbital grid system, where the population development was favoured. In those conditions, cells appeared to escape from the zone of the influence of the grids and concentrated in calmer thin layers either at the top or at the bottom of the containers. This ecophysiological study provides new evidences about the sensitivity to high levels of small-scale turbulence by two life cycle related processes, toxin production and encystment, in dinoflagellates. This can contribute to the understanding of the dynamics of those organisms in nature.


2007 ◽  
Vol 4 (2) ◽  
pp. 893-908 ◽  
Author(s):  
L. Bolli ◽  
G. Llaveria ◽  
E. Garcés ◽  
Ó. Guadayol ◽  
K. van Lenning ◽  
...  

Abstract. In some dinoflagellate species, physiological processes appear to be altered by exposure to certain turbulent conditions. Here we investigated how two levels of turbulent kinetic energy dissipation rates (ε = 0.4 and 27 cm2 s−3) affected the toxin and ecdysal cyst dynamics of two bloom forming species, Alexandrium minutum and A. catenella. The most striking responses were observed at the high ε generated by an orbital shaker. In A. catenella, lower cellular toxin content was measured in cultures shaken for more than 4 days. The same trend was observed in A. minutum, although variability masked statistical significance. For the two species, inhibition of ecdysal cyst production occurred immediately and during the period of exposure of the cultures to stirring (4 or more days) at any time during their growth curve. Recovery of cyst abundances was always observed when turbulence stopped. When turbulence persisted for more than 4 days the net growth rate significantly decreased and the final biomass yield was lower than in the unshaken cultures. This study suggests that high levels of small-scale turbulence would contribute to the modulation of the harmful bloom dynamics through the interaction at the level of toxin and encystment processes.


2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Zhuo Wang ◽  
Kun Luo ◽  
Junhua Tan ◽  
Dong Li ◽  
Jianren Fan
Keyword(s):  

2019 ◽  
Vol 4 (12) ◽  
Author(s):  
C. Marchioli ◽  
H. Bhatia ◽  
G. Sardina ◽  
L. Brandt ◽  
A. Soldati

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Hai Le-The ◽  
Christian Küchler ◽  
Albert van den Berg ◽  
Eberhard Bodenschatz ◽  
Detlef Lohse ◽  
...  

AbstractWe report a robust fabrication method for patterning freestanding Pt nanowires for use as thermal anemometry probes for small-scale turbulence measurements. Using e-beam lithography, high aspect ratio Pt nanowires (~300 nm width, ~70 µm length, ~100 nm thickness) were patterned on the surface of oxidized silicon (Si) wafers. Combining wet etching processes with dry etching processes, these Pt nanowires were successfully released, rendering them freestanding between two silicon dioxide (SiO2) beams supported on Si cantilevers. Moreover, the unique design of the bridge holding the device allowed gentle release of the device without damaging the Pt nanowires. The total fabrication time was minimized by restricting the use of e-beam lithography to the patterning of the Pt nanowires, while standard photolithography was employed for other parts of the devices. We demonstrate that the fabricated sensors are suitable for turbulence measurements when operated in constant-current mode. A robust calibration between the output voltage and the fluid velocity was established over the velocity range from 0.5 to 5 m s−1 in a SF6 atmosphere at a pressure of 2 bar and a temperature of 21 °C. The sensing signal from the nanowires showed negligible drift over a period of several hours. Moreover, we confirmed that the nanowires can withstand high dynamic pressures by testing them in air at room temperature for velocities up to 55 m s−1.


1990 ◽  
Vol 140 ◽  
pp. 133-134
Author(s):  
J. Panesar ◽  
A.H. Nelson

We report here some preliminary results of 3–D numerical simulations of an α–ω dynamo in galaxies with differential rotation, small–scale turbulence, and a shock wave induced by a stellar density wave. We obtain the magnetic field from the standard dynamo equation, but include the spiral shock velocity field from a hydrodynamic simulation of the gas flow in a gravitational field with a spiral perturbation (Johns and Nelson, 1986).


1997 ◽  
Vol 29 (1) ◽  
pp. 435-472 ◽  
Author(s):  
K. R. Sreenivasan ◽  
R. A. Antonia
Keyword(s):  

2012 ◽  
Vol 696 ◽  
pp. 122-151 ◽  
Author(s):  
Kan Wang ◽  
Meng Wang

AbstractCompressible large-eddy simulations are carried out to study the aero-optical distortions caused by Mach 0.5 flat-plate turbulent boundary layers at Reynolds numbers of ${\mathit{Re}}_{\theta } = 875$, 1770 and 3550, based on momentum thickness. The fluctuations of refractive index are calculated from the density field, and wavefront distortions of an optical beam traversing the boundary layer are computed based on geometric optics. The effects of aperture size, small-scale turbulence, different flow regions and beam elevation angle are examined and the underlying flow physics is analysed. It is found that the level of optical distortion decreases with increasing Reynolds number within the Reynolds-number range considered. The contributions from the viscous sublayer and buffer layer are small, while the wake region plays a dominant role, followed by the logarithmic layer. By low-pass filtering the fluctuating density field, it is shown that small-scale turbulence is optically inactive. Consistent with previous experimental findings, the distortion magnitude is dependent on the propagation direction due to anisotropy of the boundary-layer vortical structures. Density correlations and length scales are analysed to understand the elevation-angle dependence and its relation to turbulence structures. The applicability of Sutton’s linking equation to boundary-layer flows is examined, and excellent agreement between linking equation predictions and directly integrated distortions is obtained when the density length scale is appropriately defined.


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