scholarly journals A new method for three-dimensional immunofluorescence study of the cochlea

2020 ◽  
Vol 392 ◽  
pp. 107956
Author(s):  
Kate M. Brody ◽  
Amy J. Hampson ◽  
Hyun-jung Cho ◽  
Prudence Johnson ◽  
Stephen J. O’Leary
2021 ◽  
Vol 11 (10) ◽  
pp. 4612
Author(s):  
KweonSoo Seo ◽  
Sunjai Kim

Purpose: The aim of this study was to present a new method to analyze the three-dimensional accuracy of complete-arch dental impressions and verify the reliability of the method. Additionally, the accuracies of conventional and intraoral digital impressions were compared using the new method. Methods: A master model was fabricated using 14 milled polyetheretherketone cylinders and a maxillary acrylic model. Each cylinder was positioned and named according to its corresponding tooth position. Twenty-five definitive stone casts were fabricated using conventional impressions of the master model. An intraoral scanner was used to scan the master model 25 times to fabricate 25 digital models. A coordinate measuring machine was used to physically probe each cylinder in the master model and definitive casts. An inspection software was used to probe cylinders of digital models. A three-dimensional part coordinate system was defined and used to compute the centroid coordinate of each cylinder. Intraclass correlation coefficient (ICC) was evaluated to examine the reliability of the new method. Independent two sample t-test was performed to compare the trueness and precision of conventional and intraoral digital impressions (α = 0.05). Results: ICC results showed that, the new method had almost perfect reliability for the measurements of the master model, conventional and digital impression. Conventional impression showed more accurate absolute trueness and precision than intraoral digital impression for most of the tooth positions (p < 0.05). Conclusions: The new method was reliable to analyze the three-dimensional deviation of complete-arch impressions. Conventional impression was still more accurate than digital intraoral impression for complete arches.


Author(s):  
Matija Prka ◽  
Albert Despot ◽  
Alemka Brnčić Fischer ◽  
Herman Haller ◽  
Ana Tikvica Luetić ◽  
...  

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Javier Caviedes-Bucheli ◽  
Nestor Rios-Osorio ◽  
Diana Usme ◽  
Cristian Jimenez ◽  
Adriana Pinzon ◽  
...  

Abstract Background The purpose of this study was to evaluate the changes in canal volume after root canal preparation in vivo with 3 different single-file techniques (Reciproc-Blue®, WaveOne-Gold® and XP-EndoShaper®), with a new method using CBCT and 3D reconstruction. Methods In this prospective study, thirty human lower premolars from healthy patients were used, in which extraction was indicated for orthodontic reasons. All the teeth used were caries- and restoration-free with complete root development, without signs of periodontal disease or traumatic occlusion, and with only one straight canal (up to 25º curvature). Teeth were randomly divided into three different groups: Reciproc-Blue, WaveOne-Gold and XP-EndoShaper. CBCT scans before root canal preparation were used to create a 3D reconstruction with RHINOCEROS 5.0 software to assess the initial canal volume, and then compared with 3D reconstructions after canal preparation to measure the increase in canal volume. Student’s t test for paired data were used to determine statistically significant differences between the before and after canal volumes. Anova test was used to determine statistically significant differences in the percentage of canal volume increase between the groups and Tukey's post-hoc test were used to paired comparison. Results Reciproc-Blue showed the higher increase in canal volume, followed by WaveOne-Gold and XP-EndoShaper (p = 0.003). XP-EndoShaper did not show a statistically significant increase in canal volume after root canal preparation (p = 0.06). Conclusion With this model, Reciproc-Blue showed higher increase in root canal volume, followed by WaveOne-Gold, while XP-EndoShaper did not significantly increase root canal volume during preparation.


Author(s):  
Pasquale Memmolo ◽  
Maria Iannone ◽  
Maurizio Ventre ◽  
Paolo Antonio Netti ◽  
Andrea Finizio ◽  
...  

1984 ◽  
Author(s):  
Joan G. Moore ◽  
John Moore

The usefulness of three-dimensional flow calculations has frequently been obscured by the numerical mixing present in the calculation methods. This paper describes a new method of forming the finite difference momentum equations. The new method results in well posed equations which introduce no numerical mixing. It may be used with orthogonal or non-orthogonal grids and with uniform or highly non-uniform grid spacing. The method is demonstrated by comparing it with upwind differencing on the calculation of a simple example. It is then used in an elliptic pressure-correction calculation procedure to calculate a leading edge horseshoe vortex about a Rankine half body. The results compare well with the experimental data presented in a companion paper.


2003 ◽  
Vol 12 (2) ◽  
pp. 129-133 ◽  
Author(s):  
Peter Girman ◽  
Jan Kříž ◽  
Jozef Friedmanský ◽  
FrantišEk Saudek

Digital image analysis (DIA) is a new method in assessment of islet amount, which is expected to provide reliable and consistent results. We compared this method with conventional counting of small numbers of rat islets. Islets were isolated from 8 pancreases and counted in 24 samples in duplicate, first routinely by sizing according to estimated diameters under a calibrated reticule and then by processing of islets pictures taken by camera. As presumed, no significant difference was found in absolute numbers of islets per sample between DIA and conventional assessment. Volumes of islets per sample measured by DIA were on average more than 10% higher than amounts evaluated conventionally, which was statistically significant. DIA has been shown to be an important method to remove operator bias and provide consistent results. Evaluation of only two dimensions of three-dimensional objects still represents a certain limitation of this technique. With lowering of computer prices the system could become easily available for islet laboratories.


1992 ◽  
Vol 31 (2) ◽  
pp. 217 ◽  
Author(s):  
Masahiro Yamaguchi ◽  
Nagaaki Ohyama ◽  
Toshio Honda
Keyword(s):  

1998 ◽  
Vol 3 (2) ◽  
pp. 129-134 ◽  
Author(s):  
VILLE PULKKI

Vector base amplitude panning (VBAP) is a new method for soundscape creation. It generalises the pair-wise panning paradigm to the triplet-wise panning paradigm, which can be used in three-dimensional loudspeaker setups. Similar soundscapes can be created with differing numbers of loudspeakers in different positions. The VBAP implementation in Csound synthesis software presented in this paper is a powerful tool for producing generic soundscapes. Virtual sources are positioned by defining the virtual source directions as (azimuth, elevation) pairs; the loudspeaker setup is defined in the initial settings. Virtual sources can be moved in three different ways: they can be moved along a polyline between specified directions, angular velocities can be applied to them, or their directions can be controlled arbitrarily.


Author(s):  
Zhanjiang Wang ◽  
Xiaoqing Jin ◽  
Shuangbiao Liu ◽  
Leon M. Keer ◽  
Jian Cao ◽  
...  

This paper presents a new method of contact plasticity analysis based on Galerkin vectors to solve the eigenstresses due to eigenstrain. The influence coefficients relating eigenstrains to eigenstresses thus can be divided into four terms the one due to the eigenstrains in the full space, others due to the mirrored eigenstrains in the mirror half space. Each term can be solved fast and efficient by using the three-dimensional discrete convolution and fast Fourier transform (DC-FFT) or the three-dimensional discrete correlation and fast Fourier transform (DCR-FFT). The new method is used to analyze the contact plastic residual stresses in half space.


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