Bioremediation of HCH present in soil by the white-rot fungus Bjerkandera adusta in a slurry batch bioreactor

High-density polyethylene (HDPE) is a widely used organic polymer and an emerging pollutant, because it is very stable and nonbiodegradable. Several fungal species that produce delignifying enzymes are known to be promising degraders of recalcitrant polymers, but research on the decomposition of plastics is scarce. In this study, white rot fungus, Bjerkandera adusta TBB-03, was isolated and characterized for its ability to degrade HDPE under lignocellulose substrate treatment. Ash (Fraxinus rhynchophylla) wood chips were found to stimulate laccase production (activity was > 210 U/L after 10 days of cultivation), and subsequently used for HDPE degradation assay. After 90 days, cracks formed on the surface of HDPE samples treated with TBB-03 and ash wood chips in both liquid and solid states. Raman analysis showed that the amorphous structure of HDPE was degraded by enzymes produced by TBB-03. Overall, TBB-03 is a promising resource for the biodegradation of HDPE, and this work sheds light on further applications for fungus-based plastic degradation systems.

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Chlorometabolite production by the ecologically important white rot fungus Bjerkandera adusta

Chemosphere ◽

10.1016/s0045-6535(00)00537-3 ◽

2001 ◽

Vol 44 (7) ◽

pp. 1603-1616 ◽

Cited By ~ 15

Author(s):

P.J Silk ◽

C Aubry ◽

G.C Lonergan ◽

J.B Macaulay

Keyword(s):

White Rot ◽

White Rot Fungus ◽

Bjerkandera Adusta

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Recalcitrant Compounds Removal in Raw Leachate and Synthetic Effluents Using the White-Rot Fungus Bjerkandera adusta

Water ◽

10.3390/w9110824 ◽

2017 ◽

Vol 9 (11) ◽

pp. 824 ◽

Cited By ~ 12

Author(s):

Alessandra Bardi ◽

Qiuyan Yuan ◽

Valeria Tigini ◽

Federica Spina ◽

Giovanna Varese ◽

...

Keyword(s):

White Rot ◽

White Rot Fungus ◽

Bjerkandera Adusta ◽

Recalcitrant Compounds

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Metabolism of halogenated compounds in the white rot fungus Bjerkandera adusta studied by membrane inlet mass spectrometry and tandem mass spectrometry

Biotechnology and Bioengineering ◽

10.1002/(sici)1097-0290(19960705)51:1<23::aid-bit3>3.0.co;2-2 ◽

2000 ◽

Vol 51 (1) ◽

pp. 23-32 ◽

Cited By ~ 3

Author(s):

H. C. Beck ◽

F. R. Lauritsen ◽

J. S. Patrick ◽

R. G. Cooks

Keyword(s):

Mass Spectrometry ◽

Tandem Mass Spectrometry ◽

White Rot ◽

White Rot Fungus ◽

Tandem Mass ◽

Halogenated Compounds ◽

Bjerkandera Adusta ◽

Membrane Inlet Mass Spectrometry

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Interdependence of Primary Metabolism and Xenobiotic Mitigation Characterizes the Proteome of Bjerkandera adusta during Wood Decomposition

Applied and Environmental Microbiology ◽

10.1128/aem.01401-17 ◽

2017 ◽

Vol 84 (2) ◽

Cited By ~ 5

Author(s):

S. C. Moody ◽

E. Dudley ◽

J. Hiscox ◽

L. Boddy ◽

D. C. Eastwood

Keyword(s):

Incubation Temperature ◽

Protein Profile ◽

Nutrient Acquisition ◽

Protein Domain ◽

White Rot ◽

White Rot Fungus ◽

Primary Metabolism ◽

Bjerkandera Adusta ◽

Content Type ◽

Key Features

ABSTRACTThe aim of the current work was to identify key features of the fungal proteome involved in the active decay of beechwood blocks by the white rot fungusBjerkandera adustaat 20°C and 24°C. A combination of protein and domain analyses ensured a high level of annotation, which revealed that while the variation in the proteins identified was high between replicates, there was a considerable degree of functional conservation between the two temperatures. Further analysis revealed differences in the pathways and processes employed by the fungus at the different temperatures, particularly in relation to nutrient acquisition and xenobiotic mitigation. Key features showing temperature-dependent variation in mechanisms for both lignocellulose decomposition and sugar utilization were found, alongside differences in the enzymes involved in mitigation against damage caused by toxic phenolic compounds and oxidative stress.IMPORTANCEThis work was conducted using the wood decay fungusB. adusta, grown on solid wood blocks to closely mimic the natural environment, and gives greater insight into the proteome of an important environmental fungus during active decay. We show that a change in incubation temperature from 20°C to 24°C altered the protein profile. Proteomic studies in the field of white-rotting basidiomycetes have thus far been hampered by poor annotation of protein databases, with a large proportion of proteins simply with unknown function. This study was enhanced by extensive protein domain analysis, enabling a higher level of functional assignment and greater understanding of the proteome composition. This work revealed a strong interdependence of the primary process of nutrient acquisition and specialized metabolic processes for the detoxification of plant extractives and the phenolic breakdown products of lignocellulose.

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Biotreatment of naphthalene by PAH-acclimated pure culture with white-rot fungus phanerochaete chrysosporium

Water Science & Technology ◽

10.2166/wst.1996.0241 ◽

1996 ◽

Vol 34 (10) ◽

pp. 73-79 ◽

Cited By ~ 1

Author(s):

Liao Wan-Li ◽

Tseng Dyi-Hwa

Keyword(s):

Pure Culture ◽

Enrichment Culture ◽

Growth Characteristics ◽

White Rot ◽

White Rot Fungus ◽

High Concentration ◽

Batch Bioreactor ◽

Low Efficiency ◽

High Polarity ◽

Pah Toxicity

The tolerance of PAH toxicity for a PAH-acclimated pure culture with the fungus P. chrysosporium was evaluated by growth characteristics. The result showed that PAH-acclimated culture could still maintain its microbial activity, whereas the nonacclimated culture showed a declined growth when a high concentration of naphthalene (NTL) or benzo(a)pyrene (B(a)P) was present. In the treatment of NTL, the PAH-acclimated culture was also superior to the nonacclimated one. More than 90% of NTL removal had been attained by the PAH enrichment culture in agitated vessels within 24 h. However, in this experiment, about 20% of NTL was adsorbed on the fungal mycelia. After 4 days of reaction in the vessels, some ethyl acetate-extractable metabolites of NTL with high polarity in the HPLC elution were detected. One of these polar products was identified to be catechol compound. This study also demonstrated that NTL treatment in the aerated batch bioreactor was not comparable to that the agitated vessel owing to low efficiency in oxygen transfer of the system.

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