In vitro effect of new formulations of amphotericin B on amastigote and promastigote forms of Leishmania infantum

2007 ◽  
Vol 30 (4) ◽  
pp. 325-329 ◽  
Author(s):  
Lara Ordóñez-Gutiérrez ◽  
Raquel Espada-Fernández ◽  
María Auxiliadora Dea-Ayuela ◽  
Juan José Torrado ◽  
Francisco Bolás-Fernandez ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Leishmania Infantum ◽  
Vitro Effect

Anti-Leishmania infantum in vitro effect of n-cyclohexyl-1,2,4-oxadiazole and its ADME/TOX parameters

2021 ◽  
Author(s):  
Cristian Vicson Gomes Pinheiro ◽  
Wildson Max Barbosa da Silva ◽  
João Pedro Viana Rodrigues ◽  
Yasmim Mendes Rocha ◽  
Maria Jania Teixeira ◽  
...  
Keyword(s):  
Leishmania Infantum ◽  
Vitro Effect

scholarly journals Investigation of susceptibility to paromomycin in isolates from dogs of Leishmania (Leishmania) infantum

2019 ◽  
Author(s):  
Viviane da Luz Oliveira ◽  
Adriano Cappellazzo Coelho ◽  
Edite H. Y. Kanashiro ◽  
Mussya C. Rocha ◽  
Paulo C. Cotrim
Keyword(s):  
Side Effects ◽  
Visceral Leishmaniasis ◽  
Amphotericin B ◽  
Leishmania Infantum ◽  
Sao Paulo ◽  
Parasitic Disease ◽  
Effectiveness Rate ◽  
Alternative Drug ◽  
The City

In Brazil, visceral leishmaniasis (VL) is a parasitic disease caused by the protozoan Leishmania (Leishmania) infantum. This disease is serious and may be lethal if not treated. The treatment of leishmaniasis in Brazil consists in the use of pentavalent antimonials and/or amphotericin B. These drugs are toxic, have several side effects and the effectiveness of treatment has decreased in the last years. Paromomycin is an alternative drug already used in the treatment of VL in Asia with effectiveness rate higher than 90%. In this project, we aimed to evaluate the susceptibility in vitro to paromomycin of isolates of L. (L.) infantum from dogs of the city of Embu-Guaçu, State of São Paulo.

Antifungal effect of anthraquinones against Cryptococcus neoformans: detection of synergism with amphotericin B

2020 ◽  
Author(s):  
Raimunda Sâmia Nogueira Brilhante ◽  
Géssica dos Santos Araújo ◽  
Xhaulla Maria Quariguasi Cunha Fonseca ◽  
Glaucia Morgana de Melo Guedes ◽  
Lara de Aguiar ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Cryptococcus Neoformans ◽  
Amphotericin B ◽  
Antimicrobial Properties ◽  
Pharmacological Interaction ◽  
Minimum Inhibitory Concentrations ◽  
Broth Microdilution Method ◽  
Aloe Emodin ◽  
Vitro Effect

Abstract The emergence of tolerant Cryptococcus neoformans strains to antifungals has been described. It has directed researchers to screen for new antimicrobial compounds. In this context, several plant-derived compounds, such as anthraquinones (aloe emodin, barbaloin, and chrysophanol), have been investigated for their antimicrobial properties. This study aimed to evaluate the in vitro effect of aloe emodin, barbaloin and chrysophanol on C. neoformans in vitro growth. In addition, the interaction between these anthraquinones and amphotericin B and itraconazole was evaluated. Initially, the minimum inhibitory concentrations (MIC) of these compounds were determined against 17 strains of C. neoformans by the broth microdilution method and then pharmacological interaction assays were performed with 15 strains by the checkerboard method. Aloe emodin, barbaloin, and chrysophanol showed minimum inhibitory concentrations of 236.82–473.65 μM (64–128 μg/mL), 153–306 μM (64–128 μg/ml) and ≥1007 μM (≥256 μg/ml), respectively. Furthermore, aloe emodin (11/15), barbaloin (13/15), and chrysophanol (12/15) showed pharmacological synergism (FICI < 0.5) with amphotericin B at subinhibitory concentrations (MIC/4). The itraconazole-aloe emodin interaction was additive (1/15) (0.5 < FICI < 1.0). The itraconazole-barbaloin interaction were synergistic (2/15) and additive (5/15); whereas itraconazole-chrysophanol interactions were additive (2/15). Anthraquinones, especially aloe emodin and barbaloin, present in vitro antifungal activity against C. neoformans and potentiate the antifungal activity of amphotericin B.

The in vitro effect of nanoliposomal amphotericin B against two clinically important dermatophytes

2021 ◽  
Author(s):  
Saman Ahmad Nasrollahi ◽  
Azam Fattahi ◽  
Atefeh Naeimifar ◽  
Ensieh Lotfali ◽  
Alireza Firooz ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Vitro Effect

In vitro effect of artemether-loaded nanostructured lipid carrier (NLC) on Leishmania infantum

2021 ◽  
Author(s):  
Meisam Khazaei ◽  
Vahid Rahnama ◽  
Mohammad Hossein Motazedian ◽  
Soliman Mohammadi Samani ◽  
Gholamreza Hatam
Keyword(s):  
Leishmania Infantum ◽  
Nanostructured Lipid Carrier ◽  
Vitro Effect

In-vitro effect of itraconazole, ketoconazole and amphotericin B on the phagocytic and candidacidal function of human neutrophils

1986 ◽  
Vol 18 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Elizabeth M. Johnson ◽  
D. W. Warnock ◽  
M. D. Richardson ◽  
Candida J. Douglas
Keyword(s):  
Amphotericin B ◽  
Human Neutrophils ◽  
Vitro Effect

In vitro effect of Aloe vera, Coriandrum sativum and Ricinus communis fractions on Leishmania infantum and on murine monocytic cells

2011 ◽  
Vol 178 (3-4) ◽  
pp. 235-240 ◽  
Author(s):  
Fernanda C.M. Rondon ◽  
Claudia M.L. Bevilaqua ◽  
Marina P. Accioly ◽  
Selene M. Morais ◽  
Heitor F. Andrade-Junior ◽  
...  
Keyword(s):  
Leishmania Infantum ◽  
Ricinus Communis ◽  
Aloe Vera ◽  
Coriandrum Sativum ◽  
Monocytic Cells ◽  
Vitro Effect

scholarly journals Treatment of canine visceral leishmaniasis with Milteforan™ induces Leishmania infantum resistance to miltefosine and amphotericin B

2021 ◽  
Author(s):  
Gustavo Gonçalves ◽  
Monique Paiva Campos ◽  
Alessandra Silva Gonçalves ◽  
Lia Carolina Soares Medeiros ◽  
Fabiano Borges Figueiredo
Keyword(s):  
Visceral Leishmaniasis ◽  
Amphotericin B ◽  
Leishmania Infantum ◽  
Cross Resistance ◽  
Canine Visceral Leishmaniasis ◽  
In Vitro Susceptibility ◽  
Meglumine Antimoniate ◽  
Parasite Fitness ◽  
The Impact

Visceral leishmaniasis (VL) is the most severe form of leishmaniasis and is caused by Leishmania infantum in the Americas. Since the use of Milteforam™ was authorized to treat canine visceral leishmaniasis (CVL) in Brazil in 2017, there has also been fear of the emergence of parasites resistant to this drug and, through cross-resistance mechanisms, to meglumine antimoniate and amphotericin B. Additionally, the literature shows that acquisition of resistance is followed by increased parasite fitness, with higher rates of proliferation, infectivity and metacyclogenesis, which are determining factors for parasite virulence. In this context, this study aims to analyze the impact of treating a dog with Milteforan™ on the generation of parasites resistant to miltefosine, meglumine antimoniate, and amphotericin B. To this end, in vitro susceptibility tests were conducted against these drugs with T0 (parasites isolated from the dog before treatment with Milteforan™), T1 (after one course of treatment), and T2 (after two courses of treatment) isolates. The rates of cell proliferation, infectivity, and metacyclogenesis of the isolates were also evaluated. The results indicate a gradual increase in parasite resistance to miltefosine and amphotericin B with increasing the number of treatment courses. A trend increase in the metacyclogenesis rate of the parasites was also observed as drug resistance increased. Therefore, treatment of CVL with Milteforan™ induces resistance to miltefosine and amphotericin B as well as changes in parasite fitness, and may have an impact on animal and human public health.

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