In vitro antifungal activity of the aqueous extract of Scutellaria baicalensis Georgi root against Candida albicans

Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.

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In Vitro Antifungal Activity of (1)-N-2-Methoxybenzyl-1,10-phenanthrolinium Bromide against Candida albicans and Its Effects on Membrane Integrity

Mycobiology ◽

10.5941/myco.2017.45.1.25 ◽

2017 ◽

Vol 45 (1) ◽

pp. 25-30 ◽

Cited By ~ 2

Author(s):

Setiawati Setiawati ◽

Titik Nuryastuti ◽

Ngatidjan Ngatidjan ◽

Mustofa Mustofa ◽

Jumina Jumina ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Membrane Integrity ◽

In Vitro Antifungal Activity

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Antifungal activity of Ocimum gratissimum L., Lantana camara L. & Pteleopsis suberosa Engl. & Dies used in the treatment of vulvovaginal candidiasis in Benin

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-021-00383-4 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Jean Robert Klotoe ◽

Brice Armand Fanou ◽

Eric Agbodjento ◽

Arnaud Houehou ◽

Lauris Fah ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Aqueous Extract ◽

Lantana Camara ◽

Vulvovaginal Candidiasis ◽

Reference Drug ◽

Clinical Strains ◽

Ocimum Gratissimum

Abstract Background Vulvovaginal candidiasis is a widespread mycotic infection that affects a large proportion of women of childbearing age. Its management in traditional medicine is based on the use of medicinal plants. This study aimed to evaluate the antifungal activity of Ocimum gratissimum L., Lantana camara L. and Pteleopsis suberosa Engl. & Diels used in the treatment of vulvovaginal candidiasis in Benin. Results The data obtained from the in vitro antifungal test show that the strains tested (ATCC 90028 and two clinical strains: 1MA and 3MA) were more sensitive to aqueous extracts with a better effect for Pteleopsis suberosa. This potential of the tested extracts correlated with their richness in total polyphenols. The extract of the Pteleopsis suberosa was very active on the inhibition of the reference strain ATCC 90028. On the clinical strains (1MA and 3MA) the aqueous extract of Pteleopsis suberosa showed a better MIC on the 1MA strain. In vivo model, inoculation of 100 µL of the concentrated Candida albicans suspension 1.5 × 105 UFC/mL induced the candidiasis of the female Wistar rat. The treatment with the aqueous extract of Pteleopsis suberosa, like fluconazole (reference drug), significantly reduced Candida albicans infection at a dose of 100 mg/kg after 1, 7 and 13 days of treatment. Conclusion This study revealed the potential antifungal of the Ocimum gratissimum, Lantana camara and Pteleopsis suberosa. Pteleopsis suberosa has better antifungal activity in vitro and in vivo. These observations justify the use of their medicinal plant in the traditional treatment of vulvovaginal candidiasis in Benin.

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Antifungal Activity of Plumericin and Isoplumericin

Natural Product Communications ◽

10.1177/1934578x1100601101 ◽

2011 ◽

Vol 6 (11) ◽

pp. 1934578X1100601 ◽

Cited By ~ 1

Author(s):

Dharmendra Singh ◽

Umakant Sharma ◽

Parveen Kumar ◽

Yogesh K Gupta ◽

M. P. Dobhal ◽

...

Keyword(s):

Candida Albicans ◽

Minimum Inhibitory Concentration ◽

Antifungal Activity ◽

Cryptococcus Neoformans ◽

Inhibitory Concentration ◽

Chloroform Extract ◽

Drug Candidate ◽

Minimum Fungicidal Concentration ◽

In Vitro Antifungal Activity

This study evaluated the in vitro antifungal activity of the chloroform extract of Plumeria bicolor and its phytoconstituents plumericin and isoplumericin against Candida species and Cryptococcus neoformans by measuring the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC). Plumericin's consistently high activity against Candida albicans, C. krusei, C. glabrata, C. tropicalis and Cryptococcus neoformans was more potent than isoplumericin and the standard antifungal drug nystatin suggesting its potential as a drug candidate for candidiasis and cryptococcosis.

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In vitro Antifungal Activity of Methanolic and Chloroform Mint Leaves (Mentha piperita L.) Extracts Against Candida albicans

Journal of Physics Conference Series ◽

10.1088/1742-6596/1217/1/012136 ◽

2019 ◽

Vol 1217 ◽

pp. 012136

Author(s):

K Y Wenji ◽

I Rukmi ◽

A Suprihadi

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Mentha Piperita ◽

In Vitro Antifungal Activity

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In vitro Antifungal Activity of Dihydropyrimidinones/Thiones Against Candida albicans and Cryptococcus neoformans

Current Bioactive Compounds ◽

10.2174/1573407214666180926115745 ◽

2020 ◽

Vol 15 (6) ◽

pp. 648-655

Author(s):

Gabriel O. de Azambuja ◽

Laura Svetaz ◽

Itamar L. Gonçalves ◽

Patricia F. Corbelini ◽

Gilsane L. von Poser ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Drug Design ◽

Cryptococcus Neoformans ◽

Biginelli Reaction ◽

Fungal Growth ◽

Chemical Library ◽

Antifungal Effect ◽

In Vitro Antifungal Activity

Background: Since the Monastrol discovery in 1999 as the first inhibitor of Eg5, functionalized dihydropyrimidinones/thiones (DHPMs) have emerged as prototypes for drug design in different targets. The present work aimed to evaluate the antifungal activity of a chemical library of DHPMs. Methods: The compounds were obtained employing Biginelli reaction. Their antifungal activities were assessed against C. neoformans and C. albicans. Results: The compounds 1-i and 1-k inhibited moderately the fungal growth of C. neoformans, with compound 2-k presenting MIC80 values of 62.5-125 µg·mL-1. Considering activity against C. albicans, the compounds 1-i and 1-n present an MIC50 value of 125-250 µg·mL-1. Conclusion: The changes performed in DHPM scaffold appear to be valuable for generating compounds with potential antifungal effect.

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In Vitro Antifungal Activity and Probable Fungicidal Mechanism of Aqueous Extract of Barleria Grandiflora

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-015-1527-0 ◽

2015 ◽

Vol 175 (8) ◽

pp. 3571-3584 ◽

Cited By ~ 7

Author(s):

Suman Kumari ◽

Preeti Jain ◽

Bhawana Sharma ◽

Preeti Kadyan ◽

Rajesh Dabur

Keyword(s):

Antifungal Activity ◽

Aqueous Extract ◽

In Vitro Antifungal Activity

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In vitro antifungal activity of eugenol and vanillin against Candida albicans and Cryptococcus neoformans

Canadian Journal of Microbiology ◽

10.1139/m82-184 ◽

1982 ◽

Vol 28 (11) ◽

pp. 1235-1241 ◽

Cited By ~ 57

Author(s):

Chuenchit Boonchird ◽

T. W. Flegel

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Cryptococcus Neoformans ◽

Tube Formation ◽

Toxicity Tests ◽

Short Term ◽

Minimal Inhibitory Concentrations ◽

The Mean ◽

In Vitro Antifungal Activity

Eugenol and vanillin were examined for in vitro antifungal activity against the medically important yeasts, Candida albicans and Cryptococcus neoformans. Minimal inhibitory concentrations (MIC) and minimal fungicidal concentrations (MFC) were determined for each compound against 31 strains of C. albicans and 33 strains of C. neoformans. With eugenol, the mean MIC's for C. albicans and C. neoformans were 625 and 293 μg/mL, respectively, while the mean MFC's were 1209 and 521 μg/mL, respectively. With vanillin, the mean MIC's for C. albicans and C. neoformans were 1250 and 738 μg/mL, respectively, while the mean MFC's were 5000 and 1761 μg/mL, respectively. With C. albicans, inhibition and retardation of growth were similar for yeast and mycelial forms, but germ tube formation was inhibited at concentrations lower than those which inhibited growth. Short-term toxicity tests with mice using the intraperitoneal route gave maximum tolerated doses of 62.5 mg/kg for eugenol and 125 mg/kg for vanillin and excluded their use as therapeutic agents for systemic mycoses.

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