scholarly journals Molecular epidemiological study of Haemophilus influenzae by multilocus sequence typing

2021 ◽  
Vol 58 ◽  
pp. 21003083
Author(s):  
Saraswathiy Maniam ◽  
Fashihah Sherina Abdul Hadi Sabri ◽  
Salina Abd Sukur ◽  
Norazah Ahmad
Keyword(s):  
Haemophilus Influenzae ◽  
Epidemiological Study ◽  
Multilocus Sequence Typing ◽  
Molecular Epidemiological Study

scholarly journals Haemophilus influenzae Carriage in Children Attending French Day Care Centers: a Molecular Epidemiological Study

2003 ◽  
Vol 41 (4) ◽  
pp. 1664-1672 ◽  
Author(s):  
H. Dabernat ◽  
M.-A. Plisson-Saune ◽  
C. Delmas ◽  
M. Seguy ◽  
G. Faucon ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Epidemiological Study ◽  
Day Care ◽  
Day Care Centers ◽  
Molecular Epidemiological Study

Nonencapsulated or nontypeableHaemophilus influenzaeare more likely than their encapsulated or serotypeable counterparts to have mutations in their fucose operon

2011 ◽  
Vol 57 (12) ◽  
pp. 982-986 ◽  
Author(s):  
Michelle L. Shuel ◽  
Kathleen E. Karlowsky ◽  
Dennis K.S. Law ◽  
Raymond S.W. Tsang
Keyword(s):  
Nucleotide Sequence ◽  
Haemophilus Influenzae ◽  
Dna Sequencing ◽  
Multilocus Sequence Typing ◽  
Population Biology ◽  
Housekeeping Genes ◽  
Sequence Variations ◽  
Sequence Types ◽  
Complete Deletion

Population biology of Haemophilus influenzae can be studied by multilocus sequence typing (MLST), and isolates are assigned sequence types (STs) based on nucleotide sequence variations in seven housekeeping genes, including fucK. However, the ST cannot be assigned if one of the housekeeping genes is absent or cannot be detected by the current protocol. Occasionally, strains of H. influenzae have been reported to lack the fucK gene. In this study, we examined the prevalence of this mutation among our collection of H. influenzae isolates. Of the 704 isolates studied, including 282 encapsulated and 422 nonencapsulated isolates, nine were not typeable by MLST owing to failure to detect the fucK gene. All nine fucK-negative isolates were nonencapsulated and belonged to various biotypes. DNA sequencing of the fucose operon region confirmed complete deletion of genes in the operon in seven of the nine isolates, while in the remaining two isolates, some of the genes were found intact or in parts. The significance of these findings is discussed.

scholarly journals Molecular relationship amongSalmonella dublinisolates identified at the Center for Enterobacteriaceae of Palermo during the years 1971–85

1987 ◽  
Vol 99 (2) ◽  
pp. 283-290 ◽  
Author(s):  
A. Nastasi ◽  
M. R. Villafrate ◽  
C. Mammina ◽  
M. F. Massenti ◽  
D. Oliva ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Antibiotic Resistance ◽  
Epidemiological Study ◽  
Southern Italy ◽  
Salmonella Dublin ◽  
Genetic Function ◽  
Molecular Epidemiological Study ◽  
Plasmid Profiles ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

SUMMARYA molecular epidemiological study was carried out on 60Salmonella dublinisolates identified at the Southern Italy Enterobacteriaceae Center between 1971 and 1985. These included 23 isolates from children with diarrhoea in Palermo obtained during 1984.All isolates from the outbreak of gastroenteritis in children were resistant to chloramphenicol and streptomycin and harboured two plasmids of 50 MDa and 3 MDa molecular weight, whereas the majority of the isolates identified before 1984 were susceptible to these antibiotics and carried only a 50 MDa molecular weight plasmid. FourS. dublinstrains successively identified from cattle (Palermo, Foggia, Portici) and from a child (Palermo) were shown to possess similar antibiotic resistance patterns and plasmid profiles toS. dublinisolates from the outbreak of gastroenteritis in children.The 50 MDa plasmid was shown to be associated with virulence in mice, while it was not possible to assign any genetic function to the 3 MDa plasmid.

Molecular epidemiological study of cutaneous leishmaniasis in Beni Mellal and Fquih Ben Saleh provinces in Morocco

Acta Tropica ◽  
2015 ◽  
Vol 149 ◽  
pp. 106-112 ◽  
Author(s):  
Sebti Faiza ◽  
Hmamouch Asmae ◽  
Amarir Fatima ◽  
Fahmi Afafe ◽  
Delouane Bouchra ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Epidemiological Study ◽  
Molecular Epidemiological Study ◽  
Beni Mellal
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