Stem/progenitor cells in the postnatal inner ear of the GFP‐nestin transgenic mouse

2004 ◽  
Vol 22 (4) ◽  
pp. 205-213 ◽  
Author(s):  
Ivan A. Lopez ◽  
Paul M. Zhao ◽  
Masahiro Yamaguchi ◽  
Jean Vellis ◽  
Araceli Espinosa‐Jeffrey
Keyword(s):  
Inner Ear ◽  
Progenitor Cells ◽  
Transgenic Mouse

Inner ear progenitor cells can be generated in vitro from human bone marrow mesenchymal stem cells

2012 ◽  
Vol 7 (6) ◽  
pp. 757-767 ◽  
Author(s):  
Sarah L Boddy ◽  
Wei Chen ◽  
Ricardo Romero-Guevara ◽  
Lucksy Kottam ◽  
Illaria Bellantuono ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Inner Ear ◽  
Progenitor Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Intrinsically Self-renewing Neuroprogenitors From the A/J Mouse Spiral Ganglion as Virtually Unlimited Source of Mature Auditory Neurons

2020 ◽  
Vol 14 ◽  
Author(s):  
Francis Rousset ◽  
Vivianne B. C. Kokje ◽  
Rebecca Sipione ◽  
Dominik Schmidbauer ◽  
German Nacher-Soler ◽  
...  
Keyword(s):  
Hearing Loss ◽  
Inner Ear ◽  
Progenitor Cells ◽  
Ganglion Cells ◽  
Spiral Ganglion ◽  
Specific Cell ◽  
Auditory Neurons ◽  
Self Renewal ◽  
Starting Point ◽  
Ganglion Neurons

Nearly 460 million individuals are affected by sensorineural hearing loss (SNHL), one of the most common human sensory disorders. In mammals, hearing loss is permanent due to the lack of efficient regenerative capacity of the sensory epithelia and spiral ganglion neurons (SGN). Sphere-forming progenitor cells can be isolated from the mammalian inner ear and give rise to inner ear specific cell types in vitro. However, the self-renewing capacities of auditory progenitor cells from the sensory and neuronal compartment are limited to few passages, even after adding powerful growth factor cocktails. Here, we provide phenotypical and functional characterization of a new pool of auditory progenitors as sustainable source for sphere-derived auditory neurons. The so-called phoenix auditory neuroprogenitors, isolated from the A/J mouse spiral ganglion, exhibit robust intrinsic self-renewal properties beyond 40 passages. At any passage or freezing–thawing cycle, phoenix spheres can be efficiently differentiated into mature spiral ganglion cells by withdrawing growth factors. The differentiated cells express both neuronal and glial cell phenotypic markers and exhibit similar functional properties as mouse spiral ganglion primary explants and human sphere-derived spiral ganglion cells. In contrast to other rodent models aiming at sustained production of auditory neurons, no genetic transformation of the progenitors is needed. Phoenix spheres therefore represent an interesting starting point to further investigate self-renewal in the mammalian inner ear, which is still far from any clinical application. In the meantime, phoenix spheres already offer an unlimited source of mammalian auditory neurons for high-throughput screens while substantially reducing the numbers of animals needed.

scholarly journals Progenitor Cells from the Adult Human Inner Ear

2019 ◽  
Vol 303 (3) ◽  
pp. 461-470 ◽  
Author(s):  
Pascal Senn ◽  
Amir Mina ◽  
Stefan Volkenstein ◽  
Veronika Kranebitter ◽  
Kazuo Oshima ◽  
...  
Keyword(s):  
Inner Ear ◽  
Progenitor Cells ◽  
Adult Human ◽  
Human Inner Ear

Dkk3-Cre BAC transgenic mouse line: a tool for highly efficient gene deletion in retinal progenitor cells

genesis ◽  
2007 ◽  
Vol 45 (8) ◽  
pp. 502-507 ◽  
Author(s):  
Shigeru Sato ◽  
Tatsuya Inoue ◽  
Koji Terada ◽  
Isao Matsuo ◽  
Shinichi Aizawa ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Transgenic Mouse ◽  
Gene Deletion ◽  
Mouse Line ◽  
Retinal Progenitor Cells ◽  
Transgenic Mouse Line ◽  
Retinal Progenitor ◽  
Highly Efficient ◽  
Efficient Gene ◽  
Bac Transgenic Mouse

scholarly journals Induction of Functional Hair-Cell-Like Cells from Mouse Cochlear Multipotent Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Quanwen Liu ◽  
Yi Shen ◽  
Jiarong Chen ◽  
Jie Ding ◽  
Zihua Tang ◽  
...  
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Progenitor Cells ◽  
Stromal Cells ◽  
Cell Lineage ◽  
Membrane Properties ◽  
Patch Clamp Technique ◽  
Generating Functional ◽  
Whole Cell Patch Clamp ◽  
Multipotent Cells

In this paper, we developed a two-step-induction method of generating functional hair cells from inner ear multipotent cells. Multipotent cells from the inner ear were established and induced initially into progenitor cells committed to the inner ear cell lineage on the poly-L-lysine substratum. Subsequently, the committed progenitor cells were cultured on the mitotically inactivated chicken utricle stromal cells and induced into hair-cell-like cells containing characteristic stereocilia bundles. The hair-cell-like cells exhibited rapid permeation of FM1-43FX. The whole-cell patch-clamp technique was used to measure the membrane currents of cells differentiated for 7 days on chicken utricle stromal cells and analyze the biophysical properties of the hair-cell-like cells by recording membrane properties of cells. The results suggested that the hair-cell-like cells derived from inner ear multipotent cells were functional following differentiation in an enabling environment.

scholarly journals Lineage tracing of Sox2-expressing progenitor cells in the mouse inner ear reveals a broad contribution to non-sensory tissues and insights into the origin of the organ of Corti

2016 ◽  
Vol 414 (1) ◽  
pp. 72-84 ◽  
Author(s):  
Rende Gu ◽  
Rogers M. Brown ◽  
Chih-Wei Hsu ◽  
Tiantian Cai ◽  
Alyssa L. Crowder ◽  
...  
Keyword(s):  
Inner Ear ◽  
Progenitor Cells ◽  
Organ Of Corti ◽  
Lineage Tracing

Identification of stage-specific markers during differentiation of hair cells from mouse inner ear stem cells or progenitor cells in vitro

2015 ◽  
Vol 60 ◽  
pp. 99-111 ◽  
Author(s):  
Quanwen Liu ◽  
Jiarong Chen ◽  
Xiangli Gao ◽  
Jie Ding ◽  
Zihua Tang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Inner Ear ◽  
Progenitor Cells ◽  
Hair Cells

scholarly journals Organ of Corti size is governed by Yap/Tead-mediated progenitor self-renewal

2020 ◽  
Vol 117 (24) ◽  
pp. 13552-13561 ◽  
Author(s):  
Ksenia Gnedeva ◽  
Xizi Wang ◽  
Melissa M. McGovern ◽  
Matthew Barton ◽  
Litao Tao ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Inner Ear ◽  
Progenitor Cells ◽  
Molecular Mechanisms ◽  
Organ Of Corti ◽  
Regulatory Elements ◽  
Tissue Growth ◽  
Viral Gene ◽  
Self Renewal ◽  
Sensory Epithelia

Precise control of organ growth and patterning is executed through a balanced regulation of progenitor self-renewal and differentiation. In the auditory sensory epithelium—the organ of Corti—progenitor cells exit the cell cycle in a coordinated wave between E12.5 and E14.5 before the initiation of sensory receptor cell differentiation, making it a unique system for studying the molecular mechanisms controlling the switch between proliferation and differentiation. Here we identify the Yap/Tead complex as a key regulator of the self-renewal gene network in organ of Corti progenitor cells. We show that Tead transcription factors bind directly to the putative regulatory elements of many stemness- and cell cycle-related genes. We also show that the Tead coactivator protein, Yap, is degraded specifically in the Sox2-positive domain of the cochlear duct, resulting in down-regulation of Tead gene targets. Further, conditional loss of theYapgene in the inner ear results in the formation of significantly smaller auditory and vestibular sensory epithelia, while conditional overexpression of a constitutively active version ofYap,Yap5SA, is sufficient to prevent cell cycle exit and to prolong sensory tissue growth. We also show that viral gene delivery ofYap5SAin the postnatal inner ear sensory epithelia in vivo drives cell cycle reentry after hair cell loss. Taken together, these data highlight the key role of the Yap/Tead transcription factor complex in maintaining inner ear progenitors during development, and suggest new strategies to induce sensory cell regeneration.

Creatine supports propagation and promotes neuronal differentiation of inner ear progenitor cells

Neuroreport ◽  
2013 ◽  
pp. 1 ◽  
Author(s):  
Stefano Di Santo ◽  
Amir Mina ◽  
Angélique Ducray ◽  
Hans R. Widmer ◽  
Pascal Senn
Keyword(s):  
Inner Ear ◽  
Progenitor Cells ◽  
Neuronal Differentiation
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