In the beginning was H2+: Mass spectrometry and the molecular spectroscopy of gas-phase ions

The Electrospray Ionization (ESI) is a soft ionization technique extensively used for production of gas phase ions (without fragmentation) of thermally labile large supramolecules. In the present review we have described the development of Electrospray Ionization mass spectrometry (ESI-MS) during the last 25 years in the study of various properties of different types of biological molecules. There have been extensive studies on the mechanism of formation of charged gaseous species by the ESI. Several groups have investigated the origin and implications of the multiple charge states of proteins observed in the ESI-mass spectra of the proteins. The charged analytes produced by ESI can be fragmented by activating them in the gas-phase, and thus tandem mass spectrometry has been developed, which provides very important insights on the structural properties of the molecule. The review will highlight recent developments and emerging directions in this fascinating area of research.

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Photodissociation mass spectrometry: new tools for characterization of biological molecules

Chemical Society Reviews ◽

10.1039/c3cs60444f ◽

2014 ◽

Vol 43 (8) ◽

pp. 2757-2783 ◽

Cited By ~ 178

Author(s):

Jennifer S. Brodbelt

Keyword(s):

Mass Spectrometry ◽

Gas Phase ◽

Biological Molecules ◽

Gas Phase Ions

Fragmentation of gas-phase ions by absorption of photons affords a versatile means to characterize the structures and sequences of biological molecules.

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A mass spectrometry/mass spectrometry investigation of the nature of [C10H10]+˙, [C9H7]+ and [C10H8]+˙ gas phase ions

Organic Mass Spectrometry ◽

10.1002/oms.1210181210 ◽

1983 ◽

Vol 18 (12) ◽

pp. 542-546 ◽

Cited By ~ 15

Author(s):

Chhabil Dass ◽

M. L. Gross

Keyword(s):

Mass Spectrometry ◽

Gas Phase ◽

Gas Phase Ions ◽

Mass Spectrometry Mass Spectrometry

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Electrospray ionization mass spectrometry of the cerium(III)-phosphomolybdate complex (NH4)11Ce(PMo11O39)2

MRS Proceedings ◽

10.1557/proc-1264-z02-02 ◽

2010 ◽

Vol 1264 ◽

Author(s):

Travis Henry Bray ◽

Roy Copping ◽

David K. Shuh ◽

John Gibson

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Gas Phase ◽

Ion Trap ◽

Quadrupole Ion Trap ◽

Collision Induced Dissociation ◽

Ion Trap Mass Spectrometry ◽

Ionization Mass ◽

Naturally Occurring ◽

Gas Phase Ions

AbstractElectrospray ionization quadrupole ion trap mass spectrometry of the ammonium lanthanide(III) phosphomolybdate complex (NH4)11Ce(III)(PMo11O39)2 has been conducted revealing the Ce-POM complexes H2Ce(III)P2Mo22O753- and Ce(III)PMo11O382- as the primary Ce species in 10 mM solutions. From the complex isotopic fingerprints produced through the assembly of multiple molybdenum atoms, a transition metal with seven naturally occurring isotopes, the identities of larger ions were confirmed via successive collision induced dissociation (CID) studies of the gas phase ions. The result of these CID studies was the production of smaller ions with reduced molybdenum content, allowing for comparison between calculated and experimental isotopic distributions. CID studies also provided insights into favored fragmentation pathways. These studies provide a basis to explore speciation and ESI behavior of actinide cluster complexes.

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Development of an easily adaptable, high sensitivity source for inlet ionization

Analytical Methods ◽

10.1039/c7ay00995j ◽

2017 ◽

Vol 9 (34) ◽

pp. 4971-4978 ◽

Cited By ~ 9

Author(s):

I-Chung Lu ◽

Efstathios A. Elia ◽

Wen-Jing Zhang ◽

Milan Pophristic ◽

Ellen D. Inutan ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Phase ◽

Mass Spectrometer ◽

Condensed Phase ◽

Atmospheric Pressure ◽

High Sensitivity ◽

Inlet Ionization ◽

Gas Phase Ions ◽

The Matrix ◽

Ionization Processes

Unexpected ionization processes were recently discovered for use in mass spectrometry in which no added energy is required to convert condensed-phase molecules to gas-phase ions with ESI-like charge states by simply introducing the matrix/analyte sample into the sub-atmospheric pressure of the mass spectrometer.

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