Molecular identification and epidemiological data of Anisakis spp. (Nematoda: Anisakidae) larvae from Southeastern Pacific Ocean off Peru

The study aims to perform, for the first time, the molecular identification of anisakid larvae in commercial fish from the Southeastern Pacific Ocean off the Peru coast, and to provide data on their infection level by fishing ground, fish host, and site of infection. Fish specimens (N = 348) from the northern and the central coast of Peru were examined for parasites. The fish fillets were examined by the UV-press method. Anisakis spp. larvae (N = 305) were identified by mtDNA cox2 sequences analysis and by the ARMS-PCR of the locus nas10 nDNA. Two hundred and eighty-eight Anisakis Type I larvae corresponded to Anisakis pegreffii, whereas 17 Anisakis Type II larvae clustered in a phylogenetic lineage distinct from Anisakis physeteris deposited in GenBank, and corresponding to a phylogenetic lineage indicated as Anisakis sp. 2, previously detected in fish from both Pacific and Atlantic waters. Anisakis pegreffii was found to infect both the flesh and viscera, while Anisakis sp. 2 occurred only in the viscera. The average parasitic burden with A. pegreffii in the examined fish species from the two fishing grounds was significantly higher than that observed with Anisakis sp. 2. The results obtained contribute to improve the knowledge on the distribution and occurrence of Anisakis species in Southeastern Pacific waters and their implications in seafood safety for the local human populations.

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Description of adults and larvae of a new deep water species ofHyalinoecia(Polychaeta, Onuphidae) from the Southeastern Pacific Ocean

Journal of Natural History ◽

10.1080/00222938300770061 ◽

1983 ◽

Vol 17 (1) ◽

pp. 87-93 ◽

Cited By ~ 9

Author(s):

Franklin D. Carrasco

Keyword(s):

Pacific Ocean ◽

Deep Water ◽

Southeastern Pacific ◽

Deep Water Species ◽

Water Species

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Taxonomic reassessment of the Indo-Pacific Scytosiphonaceae (Phaeophyceae): Hydroclathrus rapanuii sp. nov. and Chnoospora minima from Easter Island, with proposal of Dactylosiphon gen. nov. and Pseudochnoospora gen. nov.

Botanica Marina ◽

10.1515/bot-2017-0089 ◽

2018 ◽

Vol 61 (1) ◽

pp. 47-64 ◽

Cited By ~ 5

Author(s):

Wilfred John E. Santiañez ◽

Erasmo C. Macaya ◽

Kyung Min Lee ◽

Ga Youn Cho ◽

Sung Min Boo ◽

...

Keyword(s):

Pacific Ocean ◽

Phylogenetic Analyses ◽

Easter Island ◽

New Genera ◽

Monotypic Genus ◽

Southeastern Pacific ◽

Molecular Phylogenetic ◽

Thin Membranes ◽

Hydroclathrus Clathratus ◽

Molecular Phylogenetic Data

AbstractA new and putatively endemic species ofHydroclathrus,Hydroclathrus rapanuii, is described from the geographically isolated Easter Island in the southeastern Pacific based on morphological and molecular phylogenetic data. It is distinguished from otherHydroclathrusby thalli of unevenly furrowed thin membranes, and angular, block-like plurangial sori. Our phylogenetic analyses indicated thatH. rapanuiiis closely related to the generitypeHydroclathrus clathratus. We also report on the morphology and phylogeny ofChnoospora minimafrom Easter I. and elsewhere in the Indo-Pacific Ocean, noting the previously unreported presence of hollow portions in its medulla. Although not collected from Easter I., we herein propose the recognition of two new genera,Dactylosiphongen. nov. andPseudochnoosporagen. nov., based on our three-gene phylogeny and their known morphologies and anatomies.Dactylosiphonis based on the three species currently assigned toColpomenia(C. bullosa,C. durvillei, andC. wynnei) that are genetically and morphologically (i.e. thalli with erect and finger-like tubes arising from a common saccate base) distinct from other members ofColpomenia. The monotypic genusPseudochnoosporais represented by the decumbent, branching, and inter-adhesive species currently known asChnoospora implexa. With the above proposals, we further increase the genus-level diversity of Scytosiphonaceae in the Indo-Pacific Ocean.

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