High-efficiency Agrobacterium rhizogenes-mediated genetic transformation in Artemisia vulgaris: Hairy root production and essential oil analysis

2013 ◽  
Vol 44 ◽  
pp. 643-652 ◽  
Author(s):  
G. Sujatha ◽  
S. Zdravković-Korać ◽  
D. Ćalić ◽  
G. Flamini ◽  
B.D. Ranjitha Kumari
Keyword(s):  
Essential Oil ◽  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
High Efficiency ◽  
Root Production ◽  
Oil Analysis ◽  
Artemisia Vulgaris

scholarly journals Evaluation for Efficient Hairy Root Production Assessed by GFP-gene Expression in Tomato Cultivars Inoculated with Agrobacterium rhizogenes MAFF07-20001.

2003 ◽  
Vol 20 (3) ◽  
pp. 257-261
Author(s):  
Takeshi FUKUMOTO ◽  
Teruo NONOMURA ◽  
Yoshinori MATSUDA ◽  
Shin-ichirou KOMAKI ◽  
Nobuyuki MORIURA ◽  
...  
Keyword(s):  
Gene Expression ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Root Production ◽  
Gfp Gene ◽  
Tomato Cultivars

Agrobacterium rhizogenes-Mediated Transformation of Hypericum tomentosum L. and Hypericum tetrapterum Fries.

2009 ◽  
Vol 64 (11-12) ◽  
pp. 864-868 ◽  
Author(s):  
Hedviga Komarovská ◽  
Annalisa Giovannini ◽  
Ján Košuth ◽  
Eva Čellárová
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Wild Type ◽  
Root Segments ◽  
Transgenic Lines ◽  
Type Strains ◽  
Hypericum Species

This is the fi rst evidence on successful Agrobacterium rhizogenes-mediated genetic transformation of two species from the genus Hypericum, H. tomentosum L. and H. tetrapterum Fries. Hairy root cultures were induced from root segments of both Hypericum species by two agropine wild-type strains of A. rhizogenes, ATCC 15834 and A4. The transgenic character of the hairy root cultures was proved by PCR amplifi cation of the rolABCD genes. In some H. tetrapterum transgenic lines aux genes were detected as well.

scholarly journals Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment

2006 ◽  
Vol 9 (4) ◽  
pp. 0-0 ◽  
Author(s):  
Vinod Kumar ◽  
Ashwani Sharma ◽  
Bellur Chayapathy Narasimha Prasad ◽  
Harishchandra Bhaskar Gururaj ◽  
Gokare Aswathanarayana Ravishankar
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Root Formation

Production of β-Carboline Alkaloids in Transformed Root Cultures of Peganum harmala L.

1990 ◽  
Vol 45 (6) ◽  
pp. 727-728 ◽  
Author(s):  
I. N. Kuzovkina ◽  
A. Gohar ◽  
I. E. Alterman
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Dry Mass ◽  
Suspension Cultures ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Peganum Harmala ◽  
Transformed Root Cultures

Abstract Peganum harmala, Hairy Root Cultures, β-Carboline Alkaloids Hairy root cultures of Peganum harmala were established by genetic transformation with Agrobacterium rhizogenes strain A4. In contrast to suspension cultures the root cultures contained high levels of β-carboline alka­ loids (1 -1.5% of dry mass).

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