The supplement of vitamin C facilitates l-lactic acid biosynthesis in Lactobacillus thermophilus A69 from sweet sorghum juice coupled with soybean hydrolysate as feedstocks

2020 ◽  
Vol 146 ◽  
pp. 112159 ◽  
Author(s):  
Xuejiao Tian ◽  
Wei Hu ◽  
Jihong Chen ◽  
Wei Zhang ◽  
Wenjian Li
Keyword(s):  
Lactic Acid ◽  
Vitamin C ◽  
Sweet Sorghum ◽  
Acid Biosynthesis ◽  
Sweet Sorghum Juice

scholarly journals Production and Purification of l-lactic Acid in Lab and Pilot Scales Using Sweet Sorghum Juice

Fermentation ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 36 ◽  
Author(s):  
Agata Olszewska-Widdrat ◽  
Maria Alexandri ◽  
José Pablo López-Gómez ◽  
Roland Schneider ◽  
Michael Mandl ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Sweet Sorghum ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Bacillus Coagulans ◽  
Pilot Scale ◽  
Environmentally Benign ◽  
Downstream Process ◽  
Sweet Sorghum Juice ◽  
Ultra Filtration

Sweet sorghum juice (SSJ) was evaluated as fermentation substrate for the production of l-lactic acid. A thermophilic Bacillus coagulans isolate was selected for batch fermentations without the use of additional nutrients. The first batch of SSJ (Batch A) resulted on higher lactic acid concentration, yield and productivity with values of 78.75 g∙L−1, 0.78 g∙g−1 and 1.77 g∙L−1 h−1, respectively. Similar results were obtained when the process was transferred into the pilot scale (50 L), with corresponding values of 73 g∙L−1, 0.70 g∙g−1 and 1.47 g∙L−1 h−1. A complete downstream process scheme was developed in order to separate lactic acid from the fermentation components. Coarse and ultra-filtration were employed as preliminary separation steps. Mono- and bipolar electrodialysis, followed by chromatography and vacuum evaporation were subsequently carried out leading to a solution containing 905.8 g∙L−1 lactic acid, with an optical purity of 98.9%. The results of this study highlight the importance of the downstream process with respect to using SSJ for lactic acid production. The proposed downstream process constitutes a more environmentally benign approach to conventional precipitation methods.

scholarly journals Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains

2015 ◽  
Vol 9 (1) ◽  
pp. 123-131 ◽  
Author(s):  
Mark S. Ou ◽  
Deepika Awasthi ◽  
Ismael Nieves ◽  
Liang Wang ◽  
John Erickson ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Sweet Sorghum ◽  
Bacillus Coagulans ◽  
Sweet Sorghum Juice ◽  
Optically Pure

scholarly journals Enhancement of Enduracidin Accumulation from Sweet Sorghum Juice via Alleviation of Oxidative Damage in Streptomyces Fungicidicus M30

2020 ◽  
Author(s):  
Lu Liu ◽  
Wei Hu ◽  
Jihong Chen ◽  
Wenjian Li ◽  
Libin Zhou
Keyword(s):  
Cell Growth ◽  
Vitamin C ◽  
Sweet Sorghum ◽  
Cell Damage ◽  
Inhibitory Effect ◽  
Antioxidant Enzyme Activities ◽  
Methyl Paraben ◽  
Sweet Sorghum Juice ◽  
Long Term Storage ◽  
Chemical Preservative

Abstract Background: Sweet sorghum juice is the preferred feedstock for enduracidin production, but the fermentation efficiency still need to be further enhanced. Oxygen-mediated microbial cell damage is an effective way to boost cell growth and metabolite accumulation in aerobic fermentation. However, this strategy on enduracidin production from sweet sorghum juice has not been reported yet. Results: In this study, enduracidin production titer was significantly enhanced when 90 mM Vitamin C was added at 4 d of fermentation. The enhanced T-AOC and antioxidant enzyme activities may help to further understand the mechanism of Vitamin C effect on regulation of enduracidin accumulation in S. fungicidicus M30. The effects of chemical preservative methyl paraben on cell growth and enduracidin production were also evaluated, and 0.04 g/L of methyl paraben had no significant inhibitory effect on cell growth and enduracidin production, indicating that the concentrated sweet sorghum juice assisted by methyl paraben for a long-term storage can be used as the substrate for enduracidin production effectively. Finally, based on the group with 0.04 g/L of methyl paraben and 90 mM Vitamin C, a final enduracidin concentration of 1.14 g/L was achieved from clarificated sweet sorghum juice by M30 strain. Conclusions: This work provided a promising strategy to enhance enduracidin production using sweet sorghum juice via adding the antioxidant to boost antioxidant capacity in S. fungicidicus.

scholarly journals Open simultaneous saccharification and fermentation of l-lactic acid by complete utilization of sweet sorghum stalk: a water-saving process

RSC Advances ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 5284-5290
Author(s):  
Yong Wang ◽  
Kai Huo ◽  
Lijuan Gao ◽  
Guoqun Zhao ◽  
Bin Wang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Sweet Sorghum ◽  
Simultaneous Saccharification And Fermentation ◽  
Water Saving ◽  
Acid Biosynthesis ◽  
Complete Utilization ◽  
Simultaneous Saccharification

Sweet sorghum stalk was completely used for l-lactic acid biosynthesis based on combined hydrolysis with saving 83.22% water.

scholarly journals Efficient Production of Lactic Acid from Sweet Sorghum Juice by a Newly Isolated Lactobacillus salivarius CGMCC 7.75

2013 ◽  
Vol 53 (3) ◽  
pp. 332-336 ◽  
Author(s):  
Quanlan Liu ◽  
Shanglong Wang ◽  
Jian-Fei Zhi ◽  
Henglei Ming ◽  
Dawei Teng
Keyword(s):  
Lactic Acid ◽  
Sweet Sorghum ◽  
Efficient Production ◽  
Lactobacillus Salivarius ◽  
Sweet Sorghum Juice
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