Molecular insights of cigarette smoke condensate-activated NLRP3 inflammasome in THP-1 cells in a stage-specific atherogenesis

2020 ◽  
Vol 88 ◽  
pp. 107013
Author(s):  
Sakshi Mehta ◽  
Veena Dhawan
Keyword(s):  
Cigarette Smoke ◽  
Nlrp3 Inflammasome ◽  
Cigarette Smoke Condensate

Multi-alkylated Polynuclear Aromatic Hydrocarbons of Tobacco Smoke: Separation and Identification

1978 ◽  
Vol 9 (4) ◽  
Author(s):  
M. E. Snook ◽  
R. F. Severson ◽  
R. F. Arrendale ◽  
H. C. Higman ◽  
O. T. Chortyk
Keyword(s):  
Liquid Chromatography ◽  
Cigarette Smoke ◽  
Aromatic Hydrocarbons ◽  
Ultra Violet ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Gel Chromatography ◽  
Cigarette Smoke Condensate ◽  
Gas And Liquid Chromatography ◽  
Silicic Acid Chromatography ◽  
Derivatives Of

AbstractThe methyl, multi-methyl, and ethyl derivatives of the polynuclear aromatic hydrocarbons (PAH) of cigarette smoke condensate (CSC) were isolated from the neutrals by silicic acid chromatography, solvent partitioning and gel chromatography. The procedure yielded a relatively pure PAH isolate amenable to further identifications. The multi-alkylated PAH were concentrated in the early gel fractions with parent and higher ring PAH found in subsequent gel fractions. It was shown that CSC is very rich in alkylated PAH, and their successful identification required extensive use of gas and liquid chromatography and ultra-violet and GC - mass spectrometric techniques. High-pressure liquid chromatography (HPLC) separated individual isomers of the alkylated PAH in complex GC peaks. PAH from indene to pentamethylchrysene were found. This report concludes our identification studies on the PAH of CSC and complements our two previous reports in this journal. Collectively, our studies have identified approximately 1000 PAH of cigarette smoke condensate and have led to the development of methods for the routine quantitation of PAH in smalI quantities of cigarette smoke condensate.

HSP60 Regulates the Cigarette Smoke-Induced Activation of TLR4-NF-κB-MyD88 Signaling Pathway and NLRP3 Inflammasome

2021 ◽  
Author(s):  
Guochun Ou ◽  
Mingmei Zhu ◽  
Yufang Huang ◽  
Wen Luo ◽  
Jie Zhao ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Cigarette Smoke ◽  
Nlrp3 Inflammasome ◽  
Myd88 Signaling

scholarly journals Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium

2015 ◽  
Vol 87 (2) ◽  
pp. 997-1005 ◽  
Author(s):  
Jinqiang Hu ◽  
Tao Wei ◽  
Siwen Sun ◽  
Aijing Zhao ◽  
Chunping Xu
Keyword(s):  
Cigarette Smoke ◽  
Carbohydrate Composition ◽  
Cigarette Smoke Condensate ◽  
Essential Factor ◽  
Standard Strain ◽  
Acetic Acid Production ◽  
Bifidobacterium Animalis ◽  
Molecular Determination

The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium. Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC). The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC. The results showed that CSC significantly decreased the growth of B. animalis as well as EPSs and acetic acid production. Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination. By comparison with control, CSC was found to be of great impact on EPSs carbohydrate composition. The molecular weight mass of Fr-I changed from 3.33×105 g/mol (without CSC) to 2.99×105 (with CSC). In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.

scholarly journals Cigarette smoke condensate and individual constituents modulate DNA methyltransferase expression in human liver cells

2015 ◽  
Vol 3 ◽  
pp. 205031211557831 ◽  
Author(s):  
Yongmei Xiao ◽  
Beverly Word ◽  
Lascelles Lyn-Cook ◽  
Beverly Lyn-Cook ◽  
George Hammons
Keyword(s):  
Cigarette Smoke ◽  
Human Liver ◽  
Dna Methyltransferase ◽  
Liver Cells ◽  
Cigarette Smoke Condensate ◽  
Human Liver Cells
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