Fabrication of a new all-in-one microfluidic dielectrophoresis integrated chip and living cell separation

Solving the mechanisms responsible for organelle behavior in vivo by same-cell correlative light and electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100120473 ◽

1992 ◽

Vol 50 (1) ◽

pp. 14-15

Author(s):

Conly L. Rieder

Keyword(s):

Electron Microscopy ◽

Quantitative Analysis ◽

Light Microscopy ◽

Living Cell ◽

Light And Electron Microscopy ◽

Time Behavior ◽

Dynamic Interactions ◽

Positive Identification ◽

Cellular Components

The behavior of many cellular components, and their dynamic interactions, can be characterized in the living cell with considerable spatial and temporal resolution by video-enhanced light microscopy (video-LM). Indeed, under the appropriate conditions video-LM can be used to determine the real-time behavior of organelles ≤ 25-nm in diameter (e.g., individual microtubules—see). However, when pushed to its limit the structures and components observed within the cell by video-LM cannot be resolved nor necessarily even identified, only detected. Positive identification and a quantitative analysis often requires the corresponding electron microcopy (EM).

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Stem cell separation in paediatric patients

Transfusion Science ◽

10.1016/s0955-3886(96)90098-3 ◽

1996 ◽

Vol 17 (4) ◽

pp. 607-610 ◽

Cited By ~ 2

Author(s):

J ZINGSEM

Keyword(s):

Stem Cell ◽

Cell Separation ◽

Paediatric Patients

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Development and study of a method for cell separation during white blood cell segmentation on images of bone marrow preparations in information and measurement systems for diagnostics of acute leukemia

Izmeritel`naya Tekhnika ◽

10.32446/0368-1025it.2020-7-68-72 ◽

2020 ◽

pp. 68-72

Author(s):

V.G. Nikitaev ◽

A.N. Pronichev ◽

V.V. Dmitrieva ◽

E.V. Polyakov ◽

A.D. Samsonova ◽

...

Keyword(s):

Bone Marrow ◽

Blood Cell ◽

Acute Leukemia ◽

White Blood Cell ◽

Cell Separation ◽

Large Scale ◽

Bone Marrow Cells ◽

Automated Analysis ◽

Cell Segmentation ◽

Measurement Systems

The issues of using of information and measurement systems based on processing of digital images of microscopic preparations for solving large-scale tasks of automating the diagnosis of acute leukemia are considered. The high density of leukocyte cells in the preparation (hypercellularity) is a feature of microscopic images of bone marrow preparations. It causes the proximity of cells to eachother and their contact with the formation of conglomerates. Measuring of the characteristics of bone marrow cells in such conditions leads to unacceptable errors (more than 50%). The work is devoted to segmentation of contiguous cells in images of bone marrow preparations. A method of cell separation during white blood cell segmentation on images of bone marrow preparations under conditions of hypercellularity of the preparation has been developed. The peculiarity of the proposed method is the use of an approach to segmentation of cell images based on the watershed method with markers. Key stages of the method: the formation of initial markers and builds the lines of watershed, a threshold binarization, shading inside the outline. The parameters of the separation of contiguous cells are determined. The experiment confirmed the effectiveness of the proposed method. The relative segmentation error was 5 %. The use of the proposed method in information and measurement systems of computer microscopy for automated analysis of bone marrow preparations will help to improve the accuracy of diagnosis of acute leukemia.

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Clinical Labeling and Imaging of Transplanted CD133+/CD34+ Stem Cells in Patients with Ischemic Heart Disease

The Heart Surgery Forum ◽

10.1532/hsf98.20111138 ◽

2012 ◽

Vol 15 (2) ◽

pp. 116 ◽

Cited By ~ 3

Author(s):

Ali Ghodsizad ◽

Viktor Bordel ◽

Brian Bruckner ◽

Mathias Loebe ◽

Gunter Fuerst ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Cell Separation ◽

Coronary Artery Bypass Surgery ◽

Iliac Crest ◽

Artery Bypass ◽

Good Manufacturing Practice ◽

Novel Method ◽

End Stage Heart Failure ◽

End Stage

The application of somatic stem cells has been shown to support the recovery of the myocardium in end-stage heart failure. A novel method for the intraoperative isolation and labeling of bone marrow-derived stem cells was established. After induction of general anesthesia, up to 400 mL of bone marrow were harvested from the posterior iliac crest and processed in the operating room under good manufacturing practice conditions by means of the automated cell-selection device Clini-MACS (Miltenyi Biotec). We subsequently injected autologous CD133<sup>+</sup> and CD34<sup>+</sup> stem cells in a predefined pattern around the laser channels in patients undergoing coronary artery bypass surgery and transmyocardial laser procedures. Intraoperative isolation and labeling is an effective cell-separation tool for the future, considering that novel cell markers can be promising new candidates for cell therapy.

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