Critical role of IL-21 in modulating TH17 and regulatory T cells in Behçet disease

2011 ◽  
Vol 128 (3) ◽  
pp. 655-664 ◽  
Author(s):  
Guillaume Geri ◽  
Benjamin Terrier ◽  
Michelle Rosenzwajg ◽  
Bertrand Wechsler ◽  
Maxime Touzot ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Critical Role ◽  
Behçet Disease ◽  
Behcet Disease

Abstract P288: Systemic Administration of an Angiotensin Type-2 Receptor Agonist Decreases Renal Regulatory T Cells

Hypertension ◽  
2017 ◽  
Vol 70 (suppl_1) ◽  
Author(s):  
Ellen E Gillis ◽  
Jennifer C Sullivan
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Critical Role ◽  
High Dose ◽  
Ang Ii ◽  
Osmotic Minipump ◽  
Angiotensin Type 2 Receptor ◽  
Angiotensin Type

There is increasing evidence supporting a critical role of the immune system in the development of hypertension. Our lab has previously reported sex differences in the renal T cell profile in both Spontaneously Hypertensive Rats (SHR) and Angiotensin II (Ang II) models of hypertension, with females having more anti-inflammatory regulatory T cells (Tregs) than males. Ang II has a well-defined role in the activation of pro-inflammatory T cells in hypertension via the angiotensin type-1 receptor (AT1R). Less is known about the role of the angiotensin type-2 receptor (AT2R) in the regulation of immune cells, although the AT2R has been shown to be cardioprotective and AT2R expression is greater in females than males. Based on the potential anti-hypertensive role of AT2Rs, we hypothesized that administration of an AT2R agonist, Compound 21 (C21), would increase renal Tregs, and this increase would be greater in females due to greater AT2R expression. Male and female SHR (10 weeks of age, n=3-4) were implanted with telemetry units for continuous monitoring of mean arterial pressure (MAP). Following 10 days of recovery, baseline MAP was recorded for 5 days. Rats were then divided into the following treatment groups: surgical controls, low dose C21 (150 ng/kg/min, sc by osmotic minipump), high dose C21 (300 ng/kg/min, sc by osmotic minipump). Kidneys were harvested after 2 weeks of treatment and flow cytometry was performed on whole kidney homogenates. MAP was not altered by C21 treatment in males (137±4 vs 134±4 vs 134±4 mmHg; n.s.) or females (128±2 vs 136±5 vs 134±4 mmHg; n.s.). Interestingly, despite having no effect on MAP, there was a significant decrease in renal CD3 + CD4 + FoxP3 + Tregs in females following both low and high doses of C21 (data expressed as % CD3 + CD4 + cells: 6±0.6 vs 3±0.6 vs 3.5±1.3 %, respectively; p=0.02). Tregs decrease in males following the high dose of C21 only (data expressed as % CD3 + CD4 + cells: 3.3±0.3 vs 3.3±0.5 vs 1.7±0.7 %, respectively; p=0.05). Total CD3 + T cells, CD3 + CD4 + T cells, and Th17 cells were not altered by C21 treatment. In conclusion, AT2R activation suppresses renal Tregs, and females are more sensitive than males. These data suggest a novel role for AT2R regulation in the kidney in hypertension.

Critical Role of IFNg in Allograft Tolerance Mediated by Foxp3+CD4+CD25+ Regulatory T Cells and the Production of Indoleamine 2, 3-dioxygenase by Graft Endothelial Cells

2007 ◽  
Vol 123 ◽  
pp. S130-S131
Author(s):  
Pamela Thebault ◽  
Michele Heslan ◽  
Thomas Condamine ◽  
Abdelhadi Saoudi ◽  
Marcello Hill ◽  
...  
Keyword(s):  
T Cells ◽  
Endothelial Cells ◽  
Regulatory T Cells ◽  
Critical Role ◽  
Indoleamine 2

Critical role of CD4+CD25+ regulatory T cells in preventing murine autoantibody-mediated thrombocytopenia

2012 ◽  
Vol 40 (4) ◽  
pp. 279-289 ◽  
Author(s):  
Tetsuya Nishimoto ◽  
Takashi Satoh ◽  
Tsutomu Takeuchi ◽  
Yasuo Ikeda ◽  
Masataka Kuwana
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Critical Role

T.17.5. Critical Role of p38 MAPK in the Activity of Adaptive Regulatory T Cells Induced by Anti-DNA Ig Peptide in (NZB×NZW)F1 Lupus Mice

2009 ◽  
Vol 131 ◽  
pp. S55
Author(s):  
Elaine Lourenco ◽  
Claudio Procaccini ◽  
Noriko Iikuni ◽  
Bevra Hahn ◽  
Antonio La Cava
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
P38 Mapk ◽  
Critical Role

scholarly journals Brucella abortus and Pregnancy in Mice: Impact of Chronic Infection on Fertility and the Role of Regulatory T Cells in Tissue Colonization

2020 ◽  
Vol 88 (10) ◽  
Author(s):  
Shakirat A. Adetunji ◽  
Denise L. Faustman ◽  
L. Garry Adams ◽  
Daniel G. Garcia-Gonzalez ◽  
Martha E. Hensel ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Brucella Abortus ◽  
Chronic Infection ◽  
Bacterial Infections ◽  
Bacterial Pathogens ◽  
Critical Role ◽  
Fine Tuning ◽  
Content Type

ABSTRACT Stealthy intracellular bacterial pathogens are known to establish persistent and sometimes lifelong infections. Some of these pathogens also have a tropism for the reproductive system, thereby increasing the risk of reproductive disease and infertility. To date, the pathogenic mechanism involved remains poorly understood. Here, we demonstrate that Brucella abortus, a notorious reproductive pathogen, has the ability to infect the nonpregnant uterus, sustain infection, and induce inflammatory changes during both acute and chronic stages of infection. In addition, we demonstrated that chronically infected mice had a significantly reduced number of pregnancies compared to naive controls. To investigate the immunologic mechanism responsible for uterine tropism, we explored the role of regulatory T cells (Tregs) in the pathogenesis of Brucella abortus infection. We show that highly suppressive CD4+FOXP3+TNFR2+ Tregs contribute to the persistence of Brucella abortus infection and that inactivation of Tregs with tumor necrosis factor receptor II (TNFR2) antagonistic antibody protected mice by significantly reducing bacterial burden both systemically and within reproductive tissues. These findings support a critical role of Tregs in the pathogenesis of persistence induced by intracellular bacterial pathogens, including B. abortus. Results from this study indicate that adverse reproductive outcomes can occur as sequelae of chronic infection in nonpregnant animals and that fine-tuning Treg activity may provide novel immunotherapeutic and prevention strategies against intracellular bacterial infections such as brucellosis.

scholarly journals An imbalance of esophageal effector and regulatory T cell subsets in experimental eosinophilic esophagitis in mice

2009 ◽  
Vol 297 (3) ◽  
pp. G550-G558 ◽  
Author(s):  
Xiang Zhu ◽  
Meiqin Wang ◽  
Caleb H. Crump ◽  
Anil Mishra
Keyword(s):  
T Cells ◽  
T Cell ◽  
Regulatory T Cells ◽  
Eosinophilic Esophagitis ◽  
Critical Role ◽  
T Cell Subsets ◽  
Activation Markers ◽  
Activated T Cell ◽  
Anti Inflammatory Cytokine

We recently reported a critical role for T cells in the induction of eosinophilic esophagitis (EE) in mice; however, the role of specific T cell subsets in disease pathogenesis is not yet understood. In the current study, we tested the hypothesis that allergen-induced EE develops in response to the disproportion of functionally different effector and regulatory T cells in the esophagus. Fluorescence-activated cell sorter analysis was performed to examine activated T cell subsets using the cell surface activation markers CD25 and CD69. A significant increase in activated CD4+ and CD4− T cells was observed in the total esophageal cells isolated from the mouse model of EE. Furthermore, an imbalance in the effector and regulatory T cells was observed in the esophagus. The esophageal CD4+CD45RBhigh effector T cells in allergen-challenged mice increased compared with saline-challenged mice (65.4 ± 3.6 × 103 to 44.8 ± 4.2 × 103), whereas CD4+CD45RBlow mostly regulatory T cells decreased in allergen-challenged mice compared with saline-challenged mice (5.8 ± 0.9 × 103 from 10.2 ± 1.7 × 103). The functional characteristics were examined by analysis of the pro- and anti-inflammatory cytokine profile of purified low and high CD4+CD45RB subsets from the spleen. Additionally, a significantly reduced interleukin (IL)-2 production by CD4+CD45RBlow cells in allergen-challenged mice compared with saline-challenged mice was observed. The reduced IL-2 in the CD4+CD45RBlow subset may be associated with reduction of CD4+CD45RBlow subset. In conclusion, our results suggest that local regulatory interaction of CD45RBhigh and CD45RBlow CD4+ T cells may be required for protective and pathogenic immunity in EE.

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