Efficient production of mutant phytase (phyA-7) derived from Selenomonas ruminantium using recombinant Escherichia coli in pilot scale

2014 ◽  
Vol 118 (3) ◽  
pp. 305-310 ◽  
Author(s):  
John Chi-Wei Lan ◽  
Chih-Kai Chang ◽  
Ho-Shing Wu
Keyword(s):  
Escherichia Coli ◽  
Pilot Scale ◽  
Recombinant Escherichia Coli ◽  
Efficient Production ◽  
Selenomonas Ruminantium

Optimized Culture Conditions for the Efficient Production of Porcine Adenylate Kinase in Recombinant Escherichia coli

2010 ◽  
Vol 162 (3) ◽  
pp. 823-829 ◽  
Author(s):  
Toru Matsui ◽  
Takashi Togari ◽  
Satoru Misawa ◽  
Tomoyuki Namihira ◽  
Naoya Shinzato ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Adenylate Kinase ◽  
Culture Conditions ◽  
Recombinant Escherichia Coli ◽  
Efficient Production

Efficient production of methionine from 2-amino-4-methylthiobutanenitrile by recombinant Escherichia coli harboring nitrilase

2014 ◽  
Vol 41 (10) ◽  
pp. 1479-1486 ◽  
Author(s):  
Li-Qun Jin ◽  
Zong-Tong Li ◽  
Zhi-Qiang Liu ◽  
Yu-Guo Zheng ◽  
Yin-Chu Shen
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Efficient Production

Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction

2002 ◽  
Vol 25 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Maass D. ◽  
Kreutzer A. ◽  
Sprenger G. ◽  
Bongaerts J. ◽  
Wubbolts M. ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pilot Scale ◽  
Recombinant Escherichia Coli ◽  
Reactive Extraction ◽  
Fed Batch ◽  
Fully Integrated

scholarly journals Enhanced Production of Carboxymethylcellulase by Recombinant Escherichia coli Strain from Rice Bran with Shifts in Optimal Conditions of Aeration Rate and Agitation Speed on a Pilot-Scale

2019 ◽  
Vol 9 (19) ◽  
pp. 4083
Author(s):  
Chung-Il Park ◽  
Jae-Hong Lee ◽  
Jianhong Li ◽  
Jin-Woo Lee
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Pilot Scale ◽  
Aeration Rate ◽  
Coli Strain ◽  
Agitation Speed ◽  
Recombinant Escherichia Coli ◽  
Optimal Conditions ◽  
E Coli ◽  
Enhanced Production

The optimal conditions including the aeration rate and agitation speed of bioreactors for the production of carboxymethylcellulase (CMCase) by a recombinant Escherichia coli KACC 91335P, expressing CMCase gene of B. velezensis A-68, were different from those for its cell growth. The enhanced production of CMCase by E. coli KACC 91335P with the conventional multistage process needs at least two bioreactors. Shifts in the optimal conditions of the aeration rate and agitation speed of the bioreactor from the cell growth of E. coli KACC 91335P to those for its production of CMCase were investigated for development of the simple and economic process with the high productivity and low cost. The production of CMCase by E. coli KACC 91335P with shifts in the optimal conditions of the aeration rate and agitation speed from the cell growth to its production of CMCase in a 100 L pilot-scale bioreactor was 1.36 times higher than that with a fixed optimal conditions of the aeration rate and agitation speed for the production of CMCase and it was even 1.54 times higher than that with a fixed optimal conditions of the aeration rate and agitation speed for cell growth. The best time for the shift in the optimal conditions was found to be the mid-log phase of cell growth. Owing to the mixed-growth-associated production of CMCase by E. coli KACC 91335P, shifts in the optimal conditions of the aeration rate and agitation speed of bioreactors from the cell growth to its production of CMCase seemed to result in relatively more cells for the participation in its production of CMCase, which in turn enhanced its production of CMCase. The process with a simple control for shifts in the aeration rate and agitation speed of a bioreactor for the enhanced production of CMCase by E. coli KACC 91335P on the pilot-scale can be directly applied to the industrial-scaled production of cellulase.

Efficient production of 2-deoxy-scyllo-inosose from d-glucose by metabolically engineered recombinant Escherichia coli

2007 ◽  
Vol 129 (3) ◽  
pp. 502-509 ◽  
Author(s):  
Takahisa Kogure ◽  
Naoki Wakisaka ◽  
Hiroaki Takaku ◽  
Masamichi Takagi
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Efficient Production
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