Construction of a recombinant Escherichia coli JM109/A-68 for production of carboxymethylcellulase and comparison of its production with its wild type, Bacillus velezensis A-68 in a pilot-scale bioreactor

2016 ◽  
Vol 21 (5) ◽  
pp. 601-611 ◽  
Author(s):  
Myung-Hwan Kim ◽  
Duk-Un Kang ◽  
Jin-Woo Lee
Keyword(s):  
Escherichia Coli ◽  
Pilot Scale ◽  
Recombinant Escherichia Coli ◽  
Wild Type ◽  
Bacillus Velezensis ◽  
Escherichia Coli Jm109

Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction

2002 ◽  
Vol 25 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Maass D. ◽  
Kreutzer A. ◽  
Sprenger G. ◽  
Bongaerts J. ◽  
Wubbolts M. ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pilot Scale ◽  
Recombinant Escherichia Coli ◽  
Reactive Extraction ◽  
Fed Batch ◽  
Fully Integrated

scholarly journals Enhanced Production of Carboxymethylcellulase by Recombinant Escherichia coli Strain from Rice Bran with Shifts in Optimal Conditions of Aeration Rate and Agitation Speed on a Pilot-Scale

2019 ◽  
Vol 9 (19) ◽  
pp. 4083
Author(s):  
Chung-Il Park ◽  
Jae-Hong Lee ◽  
Jianhong Li ◽  
Jin-Woo Lee
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Pilot Scale ◽  
Aeration Rate ◽  
Coli Strain ◽  
Agitation Speed ◽  
Recombinant Escherichia Coli ◽  
Optimal Conditions ◽  
E Coli ◽  
Enhanced Production

The optimal conditions including the aeration rate and agitation speed of bioreactors for the production of carboxymethylcellulase (CMCase) by a recombinant Escherichia coli KACC 91335P, expressing CMCase gene of B. velezensis A-68, were different from those for its cell growth. The enhanced production of CMCase by E. coli KACC 91335P with the conventional multistage process needs at least two bioreactors. Shifts in the optimal conditions of the aeration rate and agitation speed of the bioreactor from the cell growth of E. coli KACC 91335P to those for its production of CMCase were investigated for development of the simple and economic process with the high productivity and low cost. The production of CMCase by E. coli KACC 91335P with shifts in the optimal conditions of the aeration rate and agitation speed from the cell growth to its production of CMCase in a 100 L pilot-scale bioreactor was 1.36 times higher than that with a fixed optimal conditions of the aeration rate and agitation speed for the production of CMCase and it was even 1.54 times higher than that with a fixed optimal conditions of the aeration rate and agitation speed for cell growth. The best time for the shift in the optimal conditions was found to be the mid-log phase of cell growth. Owing to the mixed-growth-associated production of CMCase by E. coli KACC 91335P, shifts in the optimal conditions of the aeration rate and agitation speed of bioreactors from the cell growth to its production of CMCase seemed to result in relatively more cells for the participation in its production of CMCase, which in turn enhanced its production of CMCase. The process with a simple control for shifts in the aeration rate and agitation speed of a bioreactor for the enhanced production of CMCase by E. coli KACC 91335P on the pilot-scale can be directly applied to the industrial-scaled production of cellulase.

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