Simultaneous detection of Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma genitalium and Mycoplasma hominis by fluorescence polarization

2010 ◽  
Vol 150 (1) ◽  
pp. 41-43 ◽  
Author(s):  
Tingyi Bao ◽  
Rui Chen ◽  
Ju Zhang ◽  
Ding Li ◽  
Yanhai Guo ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Ureaplasma Urealyticum ◽  
Simultaneous Detection ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Ureaplasma Parvum

Negonorėjiniai uretritai: etiopatogenezė, klinikos, diagnostikos ir gydymo savitumai

2021 ◽  
Vol 25 (4) ◽  
pp. 259-264
Author(s):  
Simona Žilinskienė ◽  
Arūnas Petkevičius
Keyword(s):  
Chlamydia Trachomatis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Gardnerella Vaginalis ◽  
Ureaplasma Parvum

Negonorėjinis uretritas (NGU) yra dažniausia vyrų lytinių takų liga. Mokslinių tyrimų rezultatais pagrįsta, kad pagrindiniai sukėlėjai yra Chlamydia trachomatis, Mycoplasma genitalium, Ureaplasma urealyticum. Įdiegus pažangius molekulinės diagnostikos metodus, dažnai šlaplės mikrofloroje randama Mycoplasma hominis, Ureaplasma parvum, Gardnerella vaginalis ir kitų saprofitinių mikroorganizmų, kurių svarba uretritų etiopatogenezėje yra prieštaringa ir iki galo neišaiškinta. Negydytas vyrų uretritas gali sukelti sutrikimų, susijusių su reprodukcine bei lytine funkcija, ir yra viena iš pagrindinių nevaisingumo priežasčių. Šio straipsnio tikslas yra, apžvelgus mokslinę literatūrą, išanalizuoti vyrų NGU epidemiologiją, priežastis, diagnostikos ir gydymo galimybes.

scholarly journals Molecular epidemiology of non-viral sexually transmitted infections in the central Alpine province of Bolzano, northern Italy from April 2016 to March 2017

2018 ◽  
Vol 33 (1) ◽  
Author(s):  
Richard Aschbacher ◽  
Francesca Romagnoli ◽  
Elisa Masi ◽  
Valentina Pasquetto ◽  
Franco Perino ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Northern Italy ◽  
Sexually Transmitted ◽  
Ureaplasma Parvum ◽  
One Year ◽  
The One

Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium are established or presumed as (??) STI pathogens. The present study aims  at ng describing the one-year molecular epidemiology of these seven pathogens in the Province of Bolzano, Northern Italy. From April 2016 to March 2017, a total of  2,949 patients, mainly females, were enrolled and 3,427 urine, vaginal, endocervical and/or urethral samples were subjected to simultaneous analysis of the seven pathogens by means of Real Time Polymerase Chain Reaction (AnyplexTM II STI-7 Detection Kit Seegene, Seoul, Korea). At least one of the seven microorganisms was detected in 40.7% of patients, with an uneven distribution: 43.1% in females (F) and 29.8% (p<0.001) in males (M). The prevalence of microorganisms was as follows: 30.3% U. parvum (F: 35.6%, M: 8.3%), 6.9% U. urealyticum (F: 6.8%, M: 7.0%), 4.9% M. hominis (F: 5.4%, M: 2.3%), 4.9% C. trachomatis (F: 3.4%, M: 11.4%), 1.1% M. genitalium (F: 1.0%, M: 1.2%), 1.2% N. gonorrhoeae (F: 0.17%, M: 5.6%) and 0.40% T. vaginalis (F: 0.38%, M: 0.53%). Mixed infections were detected in 7.4% of patients. The highest prevalence was observed for U. parvum, followed by U. urealyticum and M. hominis and a significant  presence of multi-pathogen infections was registered.

scholarly journals In Vitro Activities of Eravacycline and Other Antimicrobial Agents against Human Mycoplasmas and Ureaplasmas

2020 ◽  
Vol 64 (8) ◽  
Author(s):  
Ken B. Waites ◽  
Donna M. Crabb ◽  
Li Xiao ◽  
Lynn B. Duffy ◽  
Sixto M. Leal
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Content Type ◽  
Ureaplasma Parvum

ABSTRACT We performed in vitro susceptibility testing for eravacycline in comparison to 4 other antimicrobials against 10 Mycoplasma genitalium, 40 Mycoplasma hominis, 44 Mycoplasma pneumoniae, 20 Ureaplasma parvum, and 20 Ureaplasma urealyticum isolates. All eravacycline MICs were ≤0.25 μg/ml, except that for one isolate of M. genitalium, for which the MIC was 2 μg/ml. Eravacycline was markedly more potent than tetracycline, azithromycin, moxifloxacin, and clindamycin against all isolates tested, which included 37 macrolide, tetracycline, and/or fluoroquinolone-resistant organisms.

scholarly journals Frequency of Sexually Transmitted Pathogens in HIV- Positive Individuals

2021 ◽  
Author(s):  
Tuğba Bozdemir ◽  
Candan Çİçek ◽  
Deniz Gökengin ◽  
Sabire Şöhret Aydemir ◽  
İmre Altuğlu ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Hiv Positive ◽  
Sexually Transmitted ◽  
Ureaplasma Parvum ◽  
Asymptomatic Individuals ◽  
Simplex Virus

Objective: Sexually transmitted infections are frequently seen and significant infections for public health. Prevention, early diagnosis and treatment of sexually transmitted infections have an important role in controlling the transmission of Human Immunodeficiency Virus (HIV). The investigation of the frequency of other sexually transmitted pathogens in asymptomatic HIV- positive individuals was aimed in the present study. Method: Vaginal and urethral swab samples were collected by 90 HIV- positive asymptomatic individuals themselves aged between 20-69 (median=36, SD=10.48) years, between September 2015 and April 2016. The samples were assessed for the presence of Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum, Herpes simplex virus type 1 and 2, and Human papillomavirus using real-time polymerase chain reaction (PCR) method. Results: At least one or more than one sexually transmitted pathogen was identified in 49 (54.4%) of 90 HIV-positive individuals. Human Papillomavirus was found in 31 (34.4%), Ureaplasma urealyticum in 20 (22.2%), Ureaplasma parvum in 15 (16.6%), Mycoplasma genitalium in 8 (8.8%), Mycoplasma hominis in 8 (8.8%), Neisseria gonorrhoeae in 5 (5.5%) and Chlamydia trachomatis in 2 (2.2%) individuals. Trichomonas vaginalis, Herpes simplex virus type 1 and 2 were not detected in any of the clinical specimens. Conclusion: Approximately 55% of 90 HIV- positive individuals were found to be positive for sexually transmitted pathogens, and . Human Papillomavirus was the most frequently detected pathogen. This condition reveals the necessity of screening even asymptomatic individuals for the presence of this pathogen. Studies are needed to increase awareness in our country on this issue.

scholarly journals Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Fabiana Pirani Carneiro ◽  
Andersen Charles Darós ◽  
Adriana Cysneiro Milhomem Darós ◽  
Tércia Maria Mendes Lousa de Castro ◽  
Marcos de Vasconcelos Carneiro ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Multiplex Pcr ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Diagnostic Methods ◽  
Pap Smears ◽  
Ureaplasma Parvum

Introduction. Despite increasing application of molecular diagnostic methods for the detection of sexually transmitted infections, the cytological findings in pap smears of patients with pathogens that can be identified only by PCR are not yet well described. The aim of this study was to describe the most common cytological features in cervical pap smears of patients with Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum detected by multiplex PCR. Methods. Cervical samples for conventional and liquid-based cytology and for multiplex PCR were collected from women ranging from 23 to 54 years old, who underwent routine screening at a gynecological Unit. Results. Multiplex PCR was positive in 36.2% of the samples: Ureaplasma parvum 14.9%, Chlamydia trachomatis 10.6%, Trichomonas vaginalis 10.6%, Mycoplasma hominis 8.5%, Ureaplasma urealyticum 4.2%, Neisseria gonorrhoeae 2.1%, and Mycoplasma genitalium (0). Multiple pathogens were observed in 12.8% of samples. Microscopic cervicitis (≥10 polymorphonuclear leukocytes/epithelial cell) and normal (predominantly lactobacillary) microbiota were the most frequent findings in the samples in which the pathogens were detected alone or in multiple infections, except for samples with Trichomonas vaginalis in which the coccobacillary microbiota was the most common. In samples with microscopic cervicitis and normal microbiota, those with at least one pathogen identified by multiplex PCR were significantly more frequent than those with no pathogen, 66.6% versus 33.3%. Conclusion. Failure to identify an inflammatory agent in pap smear with intense neutrophil exudate may suggest the presence of Ureaplasma parvum, Ureaplasma urealyticum, Chlamydia trachomatis, or Trichomonas vaginalis. A remark on the intensity of inflammation should be made in the reports of cervical pap smears so that this cytological finding can be correlated with clinical and PCR results.

scholarly journals In Vitro Activities of Gepotidacin (GSK2140944) and Other Antimicrobial Agents against Human Mycoplasmas and Ureaplasmas

2017 ◽  
Vol 61 (10) ◽  
Author(s):  
Ken B. Waites ◽  
Donna M. Crabb ◽  
Li Xiao ◽  
Lynn B. Duffy
Keyword(s):  
Topoisomerase Ii ◽  
Antimicrobial Agents ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Content Type ◽  
Topoisomerase Ii Inhibitor ◽  
Ureaplasma Parvum ◽  
Type Strains

ABSTRACTGepotidacin, a novel first-in-class triazaacenaphthylene topoisomerase II inhibitor, was tested against 85 type strains and clinical isolates ofMycoplasma pneumoniae,Mycoplasma hominis,Mycoplasma genitalium,Ureaplasma parvum, andUreaplasma urealyticumin comparison to levofloxacin, moxifloxacin, azithromycin or clindamycin, and tetracycline. Gepotidacin MIC90s (μg/ml) were 0.125 (M. pneumoniae), 0.032 (M. genitalium), 2 (M. hominis), and 8 (Ureaplasmaspecies). Gepotidacin activity was not affected by resistance to fluoroquinolones, tetracyclines, or macrolides in the strains tested. Gepotidacin merits further study for treating infections caused by these organisms.

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