Effect of Acid Tolerance Response on Recombinant Streptokinase Production in Fed-batch Cultures of Lactococcus lactis P170 Expression System

2010 ◽  
Vol 150 ◽  
pp. 397-397
Author(s):  
Karthikeyan Thillai ◽  
Guhan Jayaraman
Keyword(s):  
Lactococcus Lactis ◽  
Expression System ◽  
Acid Tolerance ◽  
Fed Batch ◽  
Acid Tolerance Response ◽  
Batch Cultures ◽  
Tolerance Response

scholarly journals HtrA Is Essential for Efficient Secretion of Recombinant Proteins by Lactococcus lactis

2008 ◽  
Vol 74 (23) ◽  
pp. 7442-7446 ◽  
Author(s):  
Kalpana Sriraman ◽  
Guhan Jayaraman
Keyword(s):  
Recombinant Protein ◽  
Lactococcus Lactis ◽  
Protein Secretion ◽  
Recombinant Proteins ◽  
Acid Tolerance ◽  
Wild Type ◽  
Acid Tolerance Response ◽  
Recombinant Protein Secretion ◽  
Tolerance Response ◽  
Secretion Of Recombinant Proteins

ABSTRACT HtrA is a unique protease on the extracellular surface of Lactococcus lactis. It is known to take part in the proteolysis of many secreted recombinant proteins, and the mutation of htrA can lead to the complete stabilization of recombinant proteins. In this work, we have shown that htrA mutation also leads to significant reduction of the efficiency of recombinant-protein secretion. We also show that the level of HtrA can be lowered by the suppression of the acid tolerance response (ATR) in L. lactis. Instead of using an L. lactis htrA mutant, the reduction of the HtrA level in wild-type recombinant cultures of L. lactis by ATR suppression may serve as a better strategy for the production of secreted recombinant proteins.

Effect offurmutation on acid-tolerance response and in vivo virulence of avian septicemicEscherichia coli

2002 ◽  
Vol 48 (5) ◽  
pp. 458-462 ◽  
Author(s):  
Chengru Zhu ◽  
Musangu Ngeleka ◽  
Andrew A Potter ◽  
Brenda J Allan
Keyword(s):  
Parent Strain ◽  
Acid Tolerance ◽  
Wild Type ◽  
Acid Tolerance Response ◽  
E Coli ◽  
Regulator Protein ◽  
In The Wild ◽  
Tolerance Response ◽  
Wild Type Parent

The Fur (ferric uptake regulator) protein is a master regulator of iron metabolism in gram-negative bacteria. In the present study, the effect of a partial deletion of the fur gene on the acid-tolerance response and in vivo virulence of avian Escherichia coli was examined. The fur mutant was unable to trigger the acid-tolerance response as observed in the wild-type parent strain. However, the mutant was as virulent as the wild-type parent strain when tested in 1-day-old chickens by subcutaneous inoculation. These data indicate that the fur gene is involved in the acid-tolerance response but not involved in the virulence of E. coli, as detected by the ability to cause septicemia in our experimental infection.Key words: E. coli, fur, acid-tolerance response.

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