Production of 3-hydroxypropionic acid via malonyl-CoA pathway using recombinant Escherichia coli strains

2012 ◽  
Vol 157 (4) ◽  
pp. 633-640 ◽  
Author(s):  
Chelladurai Rathnasingh ◽  
Subramanian Mohan Raj ◽  
Youjin Lee ◽  
Christy Catherine ◽  
Somasundar Ashok ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Malonyl Coa ◽  
Hydroxypropionic Acid

Production of 3-hydroxypropionic acid through malonyl CoA pathway from glucose by recombinant Escherichia coli BL21

2010 ◽  
Vol 150 ◽  
pp. 22-22
Author(s):  
Chelladurai Rathnasingh ◽  
J. Chrity Catherine ◽  
S. Mohan Raj ◽  
Lee You-Jin ◽  
Park Sunghoon
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Escherichia Coli Bl21 ◽  
Malonyl Coa ◽  
Hydroxypropionic Acid

Production of 3-hydroxypropionic acid from glycerol by a novel recombinant Escherichia coli BL21 strain

2008 ◽  
Vol 43 (12) ◽  
pp. 1440-1446 ◽  
Author(s):  
Subramanian Mohan Raj ◽  
Chelladurai Rathnasingh ◽  
Ji-Eun Jo ◽  
Sunghoon Park
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Escherichia Coli Bl21 ◽  
Hydroxypropionic Acid

scholarly journals Efficient Conversion of Acetate to 3-Hydroxypropionic Acid by Engineered Escherichia coli

Catalysts ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 525 ◽  
Author(s):  
Ji Lee ◽  
Sanghak Cha ◽  
Chae Kang ◽  
Geon Lee ◽  
Hyun Lim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Carbon Source ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Microbial Processes ◽  
Glyoxylate Shunt ◽  
Shunt Pathway ◽  
Malonyl Coa ◽  
Hydroxypropionic Acid

Acetate, which is an abundant carbon source, is a potential feedstock for microbial processes that produce diverse value-added chemicals. In this study, we produced 3-hydroxypropionic acid (3-HP) from acetate with engineered Escherichia coli. For the efficient conversion of acetate to 3-HP, we initially introduced heterologous mcr (encoding malonyl-CoA reductase) from Chloroflexus aurantiacus. Then, the acetate assimilating pathway and glyoxylate shunt pathway were activated by overexpressing acs (encoding acetyl-CoA synthetase) and deleting iclR (encoding the glyoxylate shunt pathway repressor). Because a key precursor malonyl-CoA is also consumed for fatty acid synthesis, we decreased carbon flux to fatty acid synthesis by adding cerulenin. Subsequently, we found that inhibiting fatty acid synthesis dramatically improved 3-HP production (3.00 g/L of 3-HP from 8.98 g/L of acetate). The results indicated that acetate can be used as a promising carbon source for microbial processes and that 3-HP can be produced from acetate with a high yield (44.6% of the theoretical maximum yield).

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