scholarly journals Bacterial community dynamics in a swine wastewater anaerobic reactor revealed by 16S rDNA sequence analysis

2015 ◽  
Vol 194 ◽  
pp. 124-131 ◽  
Author(s):  
An-Chi Liu ◽  
Chu-Yang Chou ◽  
Ling-Ling Chen ◽  
Chih-Horng Kuo
2014 ◽  
Vol 52 (12) ◽  
pp. 1056-1056
Author(s):  
Ok-Hwa Hwang ◽  
Sebastian Raveendar ◽  
Young-Ju Kim ◽  
Ji-Hun Kim ◽  
Tae-Hun Kim ◽  
...  

1998 ◽  
Vol 64 (5) ◽  
pp. 840-841 ◽  
Author(s):  
Ryuji Kondo ◽  
Manabu Komura ◽  
Shingo Hiroishi ◽  
Yoshihiko Hata

2014 ◽  
Vol 63 (2) ◽  
pp. 157-166 ◽  
Author(s):  
OZLEM GUNAY-ESIYOK ◽  
NEFISE AKCELIK ◽  
MUSTAFA AKCELIK

Lactococcus lactis strains are used commonly as starters, which contribute to desirable flavour and texture properties known as strain-specific, in dairy industry. Genomic heterogeneity of 30 L. lactis strains originating from Turkey and characterized phenotypically were investigated in this study. Plasmid profiling, PFGE and 16S rDNA sequence analyses were performed to determine the genetic variability of strains. High degree of heterogeneity was detected among the L. lactis strains. Plasmid profiles of strains showed that compared to the plasmid free control strains, namely; L. lactis subsp. lactis IL1403 and L. lactis subsp. cremoris MG1614, all tested strains carried one to ten plasmids with molecular size ranging from 1.5 to 41.5kb. The fingerprints of strains obtained by PFGE from digestion with ApaI, SmaI and I-CeuI restriction endonucleases of chromosomal DNA's were compared with each other. All strains out of four were grouped into a large cluster A with at least 44% similarity level. The other four strains formed a minor duster B, distinctively different from major cluster A. PFGE results were confirmed by 16S rDNA sequence analysis and strains included in cluster B were identified as members of different species. These results suggested that morphologic and biochemical methods should be verified by reliable molecular approaches for the purpose of strain typing. Also, PFGE was found suitable to determine genomic differentiations among inter- and intra species.


Biofouling ◽  
2010 ◽  
Vol 26 (8) ◽  
pp. 893-899 ◽  
Author(s):  
D. Inbakandan ◽  
P. Sriyutha Murthy ◽  
R. Venkatesan ◽  
S. Ajmal Khan

Author(s):  
Jeroen Heyrman ◽  
Bram Vanparys ◽  
Niall A. Logan ◽  
An Balcaen ◽  
Marina Rodríguez-Díaz ◽  
...  

A group of 42 isolates were isolated from the soil of several disused hay fields, in the Drentse A agricultural research area (The Netherlands), that were taken out of production at different times. The group represents hitherto-uncultured Bacillus lineages that have previously been found, by a non-cultural method, to be predominant in soil. The strains were subjected to a polyphasic taxonomic study, including (GTG)5-PCR, 16S rDNA sequence analysis, DNA–DNA hybridizations, DNA base-ratio determination, fatty acid analysis and morphological and biochemical characterization. By comparing the groupings obtained by (GTG)5-PCR and 16S rDNA sequence analysis, six clusters of similar strains could be recognized. A DNA–DNA relatedness study showed that these clusters represented five novel genospecies. Further analysis supported the proposal of five novel species in the genus Bacillus, namely Bacillus novalis sp. nov. (type strain IDA3307T=R-15439T=LMG 21837T=DSM 15603T), Bacillus vireti sp. nov. (type strain IDA3632T=R-15447T=LMG 21834T=DSM 15602T), Bacillus soli sp. nov. (type strain IDA0086T=R-16300T=LMG 21838T=DSM 15604T), Bacillus bataviensis sp. nov. (type strain IDA1115T=R-16315T=LMG 21833T=DSM 15601T) and Bacillus drentensis sp. nov. (type strain IDA1967T=R-16337T=LMG 21831T=DSM 15600T).


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