Aqueous–aqueous two-phase systems composed of low molecular weight of polyethylene glycols and dextrans for counter-current chromatographic purification of proteins

2006 ◽  
Vol 844 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Yoichi Shibusawa ◽  
Naoko Takeuchi ◽  
Kazusa Sugawara ◽  
Akio Yanagida ◽  
Heisaburo Shindo ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Polyethylene Glycols ◽  
Low Molecular Weight ◽  
Chromatographic Purification ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Counter Current

Application of Aqueous Two-Phase Systems for the Recovery of Bioactive Low-Molecular Weight Compounds

2014 ◽  
Vol 49 (12) ◽  
pp. 1872-1882 ◽  
Author(s):  
Jesús Simental-Martínez ◽  
Bertha Montalvo-Hernández ◽  
Marco Rito-Palomares ◽  
Jorge Benavides
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Two Phase ◽  
Low Molecular Weight Compounds ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Modeling of counter current monoclonal antibody extraction using aqueous two-phase systems

2007 ◽  
pp. 935-940 ◽  
Author(s):  
Joachim Ahmed Samatou ◽  
Annebart Engbert Wentink ◽  
Paula Alexandra J.Rosa ◽  
Ana Margarida Azevedo ◽  
Maria Raquel Aires-Barros ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Counter Current

Separation of Glycomacropeptide (a Functional Protein Material) by Aqueous Two-Phase Systems

2012 ◽  
Vol 496 ◽  
pp. 185-188 ◽  
Author(s):  
Jiang Wu ◽  
Yu Wang ◽  
Liang Huang
Keyword(s):  
Molecular Weight ◽  
Ammonium Sulfate ◽  
Protein Recovery ◽  
Sulfate Concentration ◽  
Functional Protein ◽  
Two Phase ◽  
Optimum System ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

PEG/ ammonium sulfate aqueous two-phase systems were supplied to separate GMP. It was found that PEG molecular weight, PEG concentration and ammonium sulfate concentration could influence the partitioning of GMP. The data of experiment showed that 18% (w/w) PEG6000 and 15% (w/w) ammonium sulfate were the optimum system, in which the protein recovery was 69.2%

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