A field-adapted HPLC method for determination of amodiaquine and its metabolite in whole blood dried on filter paper

2007 ◽  
Vol 859 (1) ◽  
pp. 137-140 ◽  
Author(s):  
M. Ntale ◽  
M. Mahindi ◽  
J.W. Ogwal-Okeng ◽  
L.L. Gustafsson ◽  
O. Beck
Keyword(s):  
Filter Paper ◽  
Whole Blood ◽  
Hplc Method

An evaluation of kits for measuring thyrotropin in newborns.

1984 ◽  
Vol 30 (11) ◽  
pp. 1854-1856 ◽  
Author(s):  
E A Pierson ◽  
B L Therrell ◽  
S F Franz ◽  
A J Rudenstein ◽  
P E Dziuk
Keyword(s):  
United States ◽  
Filter Paper ◽  
Whole Blood ◽  
The United States ◽  
Medical Systems ◽  
Becton Dickinson ◽  
Screening Programs ◽  
Coefficients Of Variation ◽  
Blood Specimens

Abstract Several commercial radioimmunoassay kits are now marketed specifically for determination of thyrotropin (TSH) from whole-blood specimens collected on filter paper in neonatal screening programs. We have compared five kits in use in such screening programs in the United States. The reagent kits from Becton Dickinson, Neometrics, and Nuclear Medical Systems gave similar satisfactory results. That from Pharmacia was somewhat more difficult to use and gave greater coefficients of variation. Diagnostic Products' kit appeared to perform satisfactorily, but the analytical values obtained were significantly low, which may suggest erroneous calibrator values within the kit.

A Simple HPLC Method for the Determination of Cyclosporin A in Human Whole Blood

2006 ◽  
Vol 29 (3) ◽  
pp. 391-401 ◽  
Author(s):  
Su‐Geun Yang ◽  
So‐Ra Park ◽  
Dae‐Duk Kim ◽  
Suk‐Jae Chung ◽  
Chang‐Koo Shim
Keyword(s):  
Cyclosporin A ◽  
Whole Blood ◽  
Hplc Method ◽  
Human Whole Blood

scholarly journals A Reliable Semiautomated Method for the Determination of Total Thiamine in Whole Blood by the Thiochrome Method with High-Performance Liquid Chromatography

1982 ◽  
Vol 19 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Jaap Schrijver ◽  
Andries J Speek ◽  
Jan A Klosse ◽  
Herman J M Van Rijn ◽  
Wil H P Schreurs
Keyword(s):  
Whole Blood ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Coefficients Of Variation ◽  
Analytical Recovery ◽  
On Line ◽  
Liquid Chromatographic ◽  
Hydrolysis Of

A sensitive and reliable method for the determination of total thiamine (vitamin B1) in whole blood has been developed which is suited for routine analysis. After extraction, and enzymatic hydrolysis of thiamine phosphate esters, thiamine is separated from interfering compounds by a fully automated high-performance liquid chromatographic (HPLC) system. Thiamine is detected fluorometrically as thiochrome. By calculating the concentration of thiamine on-line with the aid of a computer, it is possible to complete one analysis within four hours. Routine thiamine determinations can be carried out in a series of 120 samples within 48 hours. The within-assay and between-assay coefficients of variation of the analysis of total thiamine in whole blood were 4·2 and 4·4%, respectively. The between-assay analytical recovery of thiamine diphosphate added to blood samples was 99·9 ± 11·7% (mean ± SD). The HPLC method described has been applied also to the analysis of thiamine in plasma and erythrocytes. In agreement with other reports, it was found that about 80% of total thiamine of whole blood is present in the erythrocytes. Reference values of thiamine in whole blood of the human were found in the range 95–155 nmol/l.

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