Improvement of the purification of Saint Louis encephalitis virus NS2B-NS3 recombinant protease expressed in Escherichia coli

2008 ◽  
Vol 868 (1-2) ◽  
pp. 58-63 ◽  
Author(s):  
Boris Pastorino ◽  
Dominique Rolland ◽  
Christophe N. Peyrefitte ◽  
Nathalie Wurtz ◽  
Lionel Almeras ◽  
...  
2014 ◽  
Vol 109 (2) ◽  
pp. 197-201 ◽  
Author(s):  
María Elisa Rivarola ◽  
Laura Beatriz Tauro ◽  
Guillermo Albrieu Llinás ◽  
Marta Silvia Contigiani

2007 ◽  
Vol 13 (1) ◽  
pp. 176-178 ◽  
Author(s):  
Adriano Mondini ◽  
Izabela Lídia Soares Cardeal ◽  
Eduardo Lázaro ◽  
Silva H. Nunes ◽  
Cibele C. Moreira ◽  
...  

2014 ◽  
Vol 26 ◽  
pp. 96-97 ◽  
Author(s):  
Felipe Gonçalves Motta Maia ◽  
Juliana Helena Chávez ◽  
William Marciel de Souza ◽  
Marilia Farignoli Romeiro ◽  
Luiza Antunes de Castro-Jorge ◽  
...  

2020 ◽  
Vol 165 (9) ◽  
pp. 2079-2082
Author(s):  
Luisina Currenti ◽  
Paula Tasca ◽  
María del Pilar Díaz ◽  
Marta Contigiani ◽  
Lorena Spinsanti

2017 ◽  
Vol 87 ◽  
pp. 381-387 ◽  
Author(s):  
Carolina Colombelli Pacca ◽  
Rafael Elias Marques ◽  
José Wanderlan P. Espindola ◽  
Gevânio B.O.Oliveira Filho ◽  
Ana Cristina Lima Leite ◽  
...  

2013 ◽  
Vol 7 ◽  
pp. BBI.S13402 ◽  
Author(s):  
Anayet Hasan ◽  
Mehjabeen Hossain ◽  
Jibran Alam

Saint Louis encephalitis virus, a member of the flaviviridae subgroup, is a culex mosquito-borne pathogen. Despite severe epidemic outbreaks on several occasions, not much progress has been made with regard to an epitope-based vaccine designed for Saint Louis encephalitis virus. The envelope proteins were collected from a protein database and analyzed with an in silico tool to identify the most immunogenic protein. The protein was then verified through several parameters to predict the T-cell and B-cell epitopes. Both T-cell and B-cell immunity were assessed to determine that the protein can induce humoral as well as cell-mediated immunity. The peptide sequence from 330–336 amino acids and the sequence REYCYEATL from the position 57 were found as the most potential B-cell and T-cell epitopes, respectively. Furthermore, as an RNA virus, one important thing was to establish the epitope as a conserved one; this was also done by in silico tools, showing 63.51% conservancy. The epitope was further tested for binding against the HLA molecule by computational docking techniques to verify the binding cleft epitope interaction. However, this is a preliminary study of designing an epitope-based peptide vaccine against Saint Louis encephalitis virus; the results awaits validation by in vitro and in vivo experiments.


2011 ◽  
Vol 6 (1) ◽  
pp. 85 ◽  
Author(s):  
Camilo H. Rotela ◽  
Lorena I. Spinsanti ◽  
Mario A. Lamfri ◽  
Marta S. Contigiani ◽  
Walter R. Almirón ◽  
...  

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