Development and validation of a simple reversed-phase HPLC method for the determination of camptothecin in animal organs following administration in solid lipid nanoparticles

2012 ◽  
Vol 880 ◽  
pp. 100-107 ◽  
Author(s):  
Susana M. Martins ◽  
Thierry Wendling ◽  
Virgínia M.F. Gonçalves ◽  
Bruno Sarmento ◽  
Domingos C. Ferreira
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Solid Lipid ◽  
Development And Validation ◽  
Animal Organs

scholarly journals DEVELOPMENT AND VALIDATION OF REVERSED PHASE HPLC-PDA METHOD FOR THE QUANTIFICATION OF CHRYSIN IN SOLID LIPID NANOPARTICLES

2019 ◽  
pp. 324-328
Author(s):  
ANUJ GARG ◽  
GOPAL PRASAD AGRAWAL ◽  
SHAHADALI K.
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Limit Of Detection ◽  
Lipid Nanoparticles ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Limit Of Quantification ◽  
Solid Lipid ◽  
Relative Standard

Objective: The main aim of the present study was to develop and validate a simple, precise and accurate Reversed-Phase HPLC-PDA method for the quantitative determination of Chrysin in solid lipid nanoparticles (SLNs). Methods: The RP-HPLC-PDA system equipped with a C-18 reversed-phase column (250 × 4.6 mm, particle size 5 μm) was employed in the present study. HPLC grade methanol and water in 85:15 (v/v) ratio was selected as the mobile phase at flow rate of 1 ml/min under an ambient column oven temperature. The detection wavelength was kept at 268 nm. Validation of developed method was performed according to the ICH guidelines. Results: The developed reversed-phase HPLC-PDA method was found to be linear in the concentration range of 0.2-10 µg/ml with a correlation coefficient of 0.999. The method was also observed to be precise with % relative standard deviation (RSD) below 2%. The limit of detection and limit of quantification of this method were found to be 0.05µg/ml and 0.14µg/ml, respectively. The percent recovery of the developed method was estimated to more than 99%. Conclusion: The developed HPLC method can be utilized for the determination of Chrysin with a high degree of accuracy, precision, robustness, specificity in solid lipid nanoparticles in the presence of excipients.

DEVELOPMENT AND VALIDATION OF UV-SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF SELEGILINE HYDROCHLORIDE LOADED SOLID LIPID NANOPARTICLES

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (08) ◽  
pp. 57-60
Author(s):  
J. B Prajapati ◽  
H Rao ◽  
H Shah ◽  
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Laboratory Scale ◽  
Solid Lipid ◽  
Ich Guidelines ◽  
Development And Validation

The present paper discusses about a simple, precise and validated method for the determination of selegiline loaded solid lipid nanoparticles. The study was carried as per the parameters laid down in ICH guidelines. Maximum wavelength of selegiline in 8:2 methanol: chloroform mixture was selected at 258nm. The method was found to be linear in the range of 200μg/mL to 1000μg/mL with correlation coefficient R2 of 0.994. Method was successfully validating as per ICH guidelines. Moreover, this method was simple, sensitive and easy to apply and can be performed at laboratory scale. Hence, the proposed method can be used for analysis of determination of selegiline loaded solid lipid nanoparticles.

Development and Validation of a Reversed-Phase HPLC Method for the Determination of Deflazacort in Pharmaceutical Dosage Forms

2007 ◽  
Vol 65 (9-10) ◽  
pp. 591-594 ◽  
Author(s):  
G. M. Corrêa ◽  
L. P. Bellé ◽  
L. Bajerski ◽  
S. H. M. Borgmann ◽  
S. G. Cardoso
Keyword(s):  
Reversed Phase ◽  
Dosage Forms ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Pharmaceutical Dosage Forms ◽  
Development And Validation

scholarly journals Simultaneous HPLC determination of coumarin, cinnamyl alcohol, cinnamaldehyd, acid cinnamic, eugenol, cinnamyl acetat, acid 2-hydroxycinnamic in cinnamon using HPLC

2020 ◽  
Vol 3 (1) ◽  
pp. 11-19
Author(s):  
Ngan Le Viet ◽  
Chi Le Dinh ◽  
Hong Anh Nguyen Thi ◽  
Ngoc Nguyen Bich ◽  
Phuong Lan Nguyen Thi ◽  
...  
Keyword(s):  
Room Temperature ◽  
Ammonium Acetate ◽  
Reversed Phase ◽  
Hplc Method ◽  
Hydroxycinnamic Acid ◽  
Cinnamyl Alcohol ◽  
Reversed Phase Hplc ◽  
Sample Matrix ◽  
Development And Validation

The aim of the present study was the development and validation of a simple, precise and specific reversed phase HPLC method for the simultaneous determination of coumarin, cinnamyl alcohol, cinnamaldehyd, acid cinnamic, eugenol, cinnamyl acetat, acid 2-hydroxycinnamic in cinnamon. The substances were extracted from the sample matrix with methanol, shaken at room temperature for 30 minutes and determined by HPLC-PDA on the following conditions: column C18 sunfire (250 × 4.6 mm, 5 µm), mobile phase 30mM ammonium acetate and methanol-acetonitrile (50:50) using gradient. The recovery > 90%, the repeatability has RSD < 7.3%, the LODs and LOQs of2-hydroxycinnamic acid, coumarin, cinnamaldehyd, cinnamyl alcohol, eugenol were 1 µg/g and 3.3 µg/g, respectively; the LODs and LOQs of cinnamic acid, cinnamyl acetate were 2 µg/g and 6.7 µg/g, respectively. The method has been applied to analyze the samples.

Sign in / Sign up

Export Citation Format

Share Document