Bioanalytical method development and validation of highly selective and sensitive LC-MS/MS method for determination of Teriparatide (parathyroid hormone fragment 1-34) in human serum through direct detection of intact teriparatide molecule

2021 ◽  
pp. 123046
Author(s):  
Manoj Bob ◽  
Ravisekhar Kashibhatta ◽  
Anil Gavande ◽  
Ravi Kiran ◽  
Sandeep Jagtap ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Human Serum ◽  
Method Development ◽  
Direct Detection ◽  
Bioanalytical Method ◽  
Method Development And Validation ◽  
Development And Validation

Bioanalytical method development and validation of UV spectrophotometric method for estimation of Bumetanide spiked in human urine

2021 ◽  
Vol 11 (2) ◽  
pp. 241-248
Author(s):  
Bhavya sri Khagga ◽  
Kavya.Parelli
Keyword(s):  
Spectrophotometric Method ◽  
Human Urine ◽  
Method Development ◽  
Uv Detector ◽  
Bioanalytical Method ◽  
Method Development And Validation ◽  
Uv Visible ◽  
Development And Validation ◽  
Spiked Human Urine

The main purpose of this study was to develop a simple precise, rapid and accurate UV-visible spectrophotometric method for determination of Bumetanide in spiked human urine by extracting the Bumetanide from spiked human urine using ethyl acetate after extraction it was scanned between 200-400nm by using UV detector and its absorbance maxima was found to be 222nm.The calibration curve was linear in the range of 1-17 µg/ml. .the recovery and assay studies of bumetanide were within 93-94.85% indicating that the proposed method can be estimation of bumetanide.

Sign in / Sign up

Export Citation Format

Share Document