scholarly journals Multi-scale integrated assessment of second generation bioethanol for transport sector in the Campania Region

2019 ◽  
Vol 217 ◽  
pp. 409-422 ◽  
Author(s):  
Angelo Fierro ◽  
Annachiara Forte ◽  
Amalia Zucaro ◽  
Roberto Micera ◽  
Mario Giampietro
Keyword(s):  
Integrated Assessment ◽  
Second Generation ◽  
Transport Sector ◽  
Campania Region ◽  
Multi Scale ◽  
Second Generation Bioethanol

scholarly journals The Availability and Assessment of Potential Agricultural Residues for the Regional Development of Second-Generation Bioethanol in Thailand

2021 ◽  
Author(s):  
Piradee Jusakulvijit ◽  
Alberto Bezama ◽  
Daniela Thrän
Keyword(s):  
Second Generation ◽  
Agricultural Sector ◽  
National Level ◽  
Value Added ◽  
Agricultural Residues ◽  
Transport Sector ◽  
Second Generation Bioethanol ◽  
Fuel Demand ◽  
Future Value ◽  
Production Capacities

AbstractThe agricultural sector is a major source of biomass feedstocks for biofuels. Even though biomass potential in Thailand has been analyzed on a national level, its distribution and potential have yet to be assessed on a regional and provincial scale. Therefore, the study aims to verify the possibility of decentralized second-generation bioethanol production from regionally available agricultural residues. Most of the generated residues stem from the country’s major crops (sugarcane, cassava, rice and palm), totaling 174.1 million tons per year. The volume of bioethanol from these residues is projected to be 20,213.5 million liters per year, meeting 31.2% of the overall fuel demand of the transport sector. At the regional level, the northeast produces the highest amount of bioethanol at 9099.7 million liters per year, followed by the central, northern and southern regions. In terms of provincial distribution, the highest amount of bioethanol is converted in Nakhon Ratchasima, amounting to 1328 million liters per year. Data from the top ten potential provinces suggest that decentralizing production facilities is possible. One of the hotspots is Surat Thani in the south which can potentially utilize palm residues as feedstocks. This regionalized assessment also found that conventional feedstocks could be substituted with regionally available residues in the 26 production plants currently in operation. The results confirm that there would be enough alternative regional feedstocks to meet existing production capacities and they indicate that there would be enough regional residues left over for future value-added utilization. Graphic Abstract

scholarly journals Identification of the major fermentation inhibitors of recombinant 2G yeasts in diverse lignocellulose hydrolysates

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Gert Vanmarcke ◽  
Mekonnen M. Demeke ◽  
Maria R. Foulquié-Moreno ◽  
Johan M. Thevelein
Keyword(s):  
Second Generation ◽  
Industrial Process ◽  
Superior Performance ◽  
Fermentation Inhibitors ◽  
Inhibitor Tolerance ◽  
Xylose Consumption ◽  
Process Economics ◽  
Partial Inhibition ◽  
Second Generation Bioethanol ◽  
Lignocellulose Hydrolysates

Abstract Background Presence of inhibitory chemicals in lignocellulose hydrolysates is a major hurdle for production of second-generation bioethanol. Especially cheaper pre-treatment methods that ensure an economical viable production process generate high levels of these inhibitory chemicals. The effect of several of these inhibitors has been extensively studied with non-xylose-fermenting laboratory strains, in synthetic media, and usually as single inhibitors, or with inhibitor concentrations much higher than those found in lignocellulose hydrolysates. However, the relevance of individual inhibitors in inhibitor-rich lignocellulose hydrolysates has remained unclear. Results The relative importance for inhibition of ethanol fermentation by two industrial second-generation yeast strains in five lignocellulose hydrolysates, from bagasse, corn cobs and spruce, has now been investigated by spiking higher concentrations of each compound in a concentration range relevant for industrial hydrolysates. The strongest inhibition was observed with industrially relevant concentrations of furfural causing partial inhibition of both D-glucose and D-xylose consumption. Addition of 3 or 6 g/L furfural strongly reduced the ethanol titer obtained with strain MD4 in all hydrolysates evaluated, in a range of 34 to 51% and of 77 to 86%, respectively. This was followed by 5-hydroxymethylfurfural, acetic acid and formic acid, for which in general, industrially relevant concentrations caused partial inhibition of D-xylose fermentation. On the other hand, spiking with levulinic acid, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid or vanillin caused little inhibition compared to unspiked hydrolysate. The further evolved MD4 strain generally showed superior performance compared to the previously developed strain GSE16-T18. Conclusion The results highlight the importance of individual inhibitor evaluation in a medium containing a genuine mix of inhibitors as well as the ethanol that is produced by the fermentation. They also highlight the potential of increasing yeast inhibitor tolerance for improving industrial process economics.

Ionic Liquid 1-Butyl-3-Methylimidazolium Chloride/FeCl3 Pretreatment of Sugarcane Residue for Second Generation Bioethanol Production

2013 ◽  
Vol 275-277 ◽  
pp. 1662-1665 ◽  
Author(s):  
Qiang Li ◽  
Juan Juan Fei ◽  
Xu Ding Gu ◽  
Geng Sheng Ji ◽  
Yang Liu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Enzymatic Hydrolysis ◽  
Low Temperature ◽  
Second Generation ◽  
Reducing Sugars ◽  
Enzymatic Saccharification ◽  
Cellulosic Biomass ◽  
Candida Shehatae ◽  
Second Generation Bioethanol ◽  
Temperature Pretreatment

This study aims to establish a natural cellulosic biomass pretreatment process using ionic liquid (IL) for efficient enzymatic hydrolysis and second generation bioethanol. The IL 1-Butyl-3-methylimidazolium Chloride/FeCl3 ([Bmim]Cl/FeCl3) was selected in view of its low temperature pretreatment ability and the potential of accelerating enzymatic hydrolysis, and it could be recyclable. The yield of reducing sugars from sugarcane residue pretreated with this IL at 80 oC for 1 h reached 46.8% after being enzymatically hydrolyzed for 24 h. Sugarcane residue regenerated were hydrolyzed more easily than that treated with water. The fermentability of the hydrolyzates, obtained after enzymatic saccharification of the regenerated sugarcane residue, was transformed into bioethanol using Candida shehatae. This microbe could absorb glucose and xylose efficiently, and the ethanol production was 0.38 g/g glucose within 30 h fermentation. In conclusion, the metal ionic liquid pretreatment in low temperature shows promise as pretreatment solvent for natural biomass.

scholarly journals Exploring grape marc as trove for new thermotolerant and inhibitor-tolerant Saccharomyces cerevisiae strains for second-generation bioethanol production

2013 ◽  
Vol 6 (1) ◽  
pp. 168 ◽  
Author(s):  
Lorenzo Favaro ◽  
Marina Basaglia ◽  
Alberto Trento ◽  
Eugéne Van Rensburg ◽  
Maria García-Aparicio ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Second Generation ◽  
Bioethanol Production ◽  
Grape Marc ◽  
Second Generation Bioethanol

scholarly journals Sustainable Second-Generation Bioethanol Production from Enzymatically Hydrolyzed Domestic Food Waste Using Pichia anomala as Biocatalyst

2020 ◽  
Vol 13 (1) ◽  
pp. 259
Author(s):  
Ioanna Ntaikou ◽  
Georgia Antonopoulou ◽  
Gerasimos Lyberatos
Keyword(s):  
Food Waste ◽  
Second Generation ◽  
Scale Up ◽  
Pichia Anomala ◽  
Small Scale ◽  
Amylolytic Enzymes ◽  
Second Generation Bioethanol ◽  
Saccharification Efficiency ◽  
Ethanol Yields ◽  
Domestic Food

In the current study, a domestic food waste containing more than 50% of carbohydrates was assessed as feedstock to produce second-generation bioethanol. Aiming to the maximum exploitation of the carbohydrate fraction of the waste, its hydrolysis via cellulolytic and amylolytic enzymatic blends was investigated and the saccharification efficiency was assessed in each case. Fermentation experiments were performed using the non-conventional yeast Pichia anomala (Wickerhamomyces anomalus) under both separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) modes to evaluate the conversion efficiencies and ethanol yields for different enzymatic loadings. It was shown that the fermentation efficiency of the yeast was not affected by the fermentation mode and was high for all handlings, reaching 83%, whereas the enzymatic blend containing the highest amount of both cellulolytic and amylolytic enzymes led to almost complete liquefaction of the waste, resulting also in ethanol yields reaching 141.06 ± 6.81 g ethanol/kg waste (0.40 ± 0.03 g ethanol/g consumed carbohydrates). In the sequel, a scale-up fermentation experiment was performed with the highest loading of enzymes in SHF mode, from which the maximum specific growth rate, μmax, and the biomass yield, Yx/s, of the yeast from the hydrolyzed waste were estimated. The ethanol yields that were achieved were similar to those of the respective small scale experiments reaching 138.67 ± 5.69 g ethanol/kg waste (0.40 ± 0.01 g ethanol/g consumed carbohydrates).

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