Replicative multivirus infection with cytomegalovirus, herpes simplex virus 1, and parvovirus B19, and latent Epstein–Barr virus infection in the synovial tissue of a psoriatic arthritis patient

2004 ◽  
Vol 31 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Yasmin Mehraein ◽  
Carsten Lennerz ◽  
Sandra Ehlhardt ◽  
Klaus D Zang ◽  
Henning Madry
Keyword(s):  
Psoriatic Arthritis ◽  
Herpes Simplex ◽  
Epstein Barr Virus ◽  
Parvovirus B19 ◽  
Epstein Barr Virus Infection ◽  
Herpes Simplex Virus 1 ◽  
Barr Virus ◽  
Epstein Barr ◽  
Barr Virus Infection ◽  
Simplex Virus

scholarly journals Acute Parvovirus B19 Infection Frequently Causes False-Positive Results in Epstein-Barr Virus- and Herpes Simplex Virus-Specific Immunoglobulin M Determinations Done on the Liaison Platform

2008 ◽  
Vol 16 (3) ◽  
pp. 372-375 ◽  
Author(s):  
Mario Berth ◽  
Eugene Bosmans
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Positive Result ◽  
Epstein Barr Virus ◽  
Parvovirus B19 ◽  
Immunoglobulin M ◽  
Barr Virus ◽  
Epstein Barr ◽  
Simplex Virus ◽  
Assay Interference

ABSTRACT During an outbreak of parvovirus B19 we collected serum samples from 68 nonpregnant patients in the region of Antwerp (Belgium). Fifty-seven (84%) of the parvovirus B19 immunoglobulin M (IgM)-positive sera had a positive result for Epstein-Barr virus (EBV) IgM by Liaison testing, 61 (90%) had a positive result for herpes simplex virus (HSV) IgM, 20 (29%) samples were positive for cytomegalovirus IgM, and 15 (22%) had a positive result for Borrelia burgdorferi sensu lato IgM. As assay interference was suspected, sera were further investigated by using additional infectious-disease serology tests and by performing various interference elimination procedures. We could show that the EBV IgM and HSV IgM results were false positives due to aspecific IgM reactions with the solid phase. All samples were also analyzed by a modified Liaison EBV IgM assay, based on the addition of polyvinylpyrrolidone and polyvinyl alcohol to the dilution buffer, which partially eliminated this type of assay interference. Although the Liaison is a very convenient, automated immunoassay platform, this study demonstrates the potential for improvement of mainly the EBV IgM and HSV IgM tests.

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