Evaluation of DNA extraction methods for dried blood spots in the diagnosis of congenital cytomegalovirus infection

2009 ◽  
Vol 46 ◽  
pp. S37-S42 ◽  
Author(s):  
Jutte J.C. de Vries ◽  
Eric C.J. Claas ◽  
Aloys C.M. Kroes ◽  
Ann C.T.M. Vossen
Keyword(s):  
Dna Extraction ◽  
Cytomegalovirus Infection ◽  
Dried Blood Spots ◽  
Extraction Methods ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus ◽  
Blood Spots ◽  
Dna Extraction Methods

Disease burden of congenital cytomegalovirus infection at school entry age: study design, participation rate and birth prevalence

2015 ◽  
Vol 144 (7) ◽  
pp. 1520-1527 ◽  
Author(s):  
M. J. KORNDEWAL ◽  
A. C. T. M. VOSSEN ◽  
J. CREMER ◽  
R. S. VAN BINNENDIJK ◽  
A. C. M. KROES ◽  
...  
Keyword(s):  
Cytomegalovirus Infection ◽  
Participation Rate ◽  
Dried Blood Spots ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus ◽  
Birth Prevalence ◽  
Blood Spots ◽  
Health And Development ◽  
Polymerase Chain

SUMMARYCongenital cytomegalovirus infection (cCMV) may lead to symptoms at birth and long-term consequences. We present a nationwide, retrospective cohort study on the outcome of cCMV up to age 6 years. For this study we identified cCMV, using polymerase chain reaction, by analysing dried blood spots, which are taken shortly after birth for neonatal screening. The group of children with cCMV were compared to a group of children who were cCMV negative at birth. Data were collected about their health and development up to age 6 years. Parents of 73 693 children were invited to participate, and 32 486 (44·1%) gave informed consent for testing of their child's dried blood spot for CMV. Of the 31 484 dried blood spots tested, 156 (0·5%) were positive for cCMV. Of these, four (2·6%) children had been diagnosed with cCMV prior to this study. This unique retrospective nationwide study permits the estimation of long-term sequelae of cCMV up to the age of 6 years. The birth prevalence of cCMV in this study was 0·5%, which is in line with prior estimates. Most (97·4%) children with cCMV had not been diagnosed earlier, indicating under-diagnosis of cCMV.

scholarly journals Evaluation of DNA extraction methods for the detection of Cytomegalovirus in dried blood spots

2015 ◽  
Vol 66 ◽  
pp. 95-99 ◽  
Author(s):  
D. Koontz ◽  
K. Baecher ◽  
M. Amin ◽  
S. Nikolova ◽  
M. Gallagher ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Dried Blood Spots ◽  
Extraction Methods ◽  
Blood Spots ◽  
Dna Extraction Methods
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