Clinical diagnosis of early dengue infection by novel one-step multiplex real-time RT-PCR targeting NS1 gene

2015 ◽  
Vol 65 ◽  
pp. 11-19 ◽  
Author(s):  
Je-Hyoung Kim ◽  
Chom-Kyu Chong ◽  
Mangalam Sinniah ◽  
Jeyaindran Sinnadurai ◽  
Hyun-Ok Song ◽  
...  
Keyword(s):  
Real Time ◽  
Clinical Diagnosis ◽  
Dengue Infection ◽  
Rt Pcr ◽  
One Step ◽  
Ns1 Gene ◽  
Pcr Targeting

scholarly journals Fast, Sensitive and Specific Detection of Thailand orthohantavirus and its Variants Using One-Step Real-Time Reverse-Transcription Polymerase Chain Reaction Assay

Viruses ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 718
Author(s):  
Vololoniaina Raharinosy ◽  
Jean-Michel Heraud ◽  
Harinirina Aina Rabemananjara ◽  
Sandra Telfer ◽  
Danielle Aurore Doll Rakoto ◽  
...  
Keyword(s):  
Real Time ◽  
Rt Pcr ◽  
Specific Detection ◽  
Chain Reaction ◽  
South West ◽  
West Part ◽  
One Step ◽  
The Indian Ocean ◽  
Polymerase Chain ◽  
Pcr Targeting

Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.

First generic one step real-time Taqman RT-PCR targeting the RNA1 of betanodaviruses

2015 ◽  
Vol 211 ◽  
pp. 1-7 ◽  
Author(s):  
M. Baud ◽  
J. Cabon ◽  
A. Salomoni ◽  
A. Toffan ◽  
V. Panzarin ◽  
...  
Keyword(s):  
Real Time ◽  
Rt Pcr ◽  
One Step ◽  
Pcr Targeting

scholarly journals Monitoring epidemic viral respiratory infections using one-step real-Time Triplex RT-PCR targeting influenza A and B viruses and respiratory syncytial virus

2011 ◽  
Vol 83 (4) ◽  
pp. 695-701 ◽  
Author(s):  
Solesne Papillard-Marechal ◽  
Vincent Enouf ◽  
Aurélie Schnuriger ◽  
Astrid Vabret ◽  
Edouard Macheras ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Real Time ◽  
Influenza A ◽  
Respiratory Infections ◽  
Rt Pcr ◽  
One Step ◽  
Syncytial Virus ◽  
Viral Respiratory Infections ◽  
Pcr Targeting ◽  
Viral Respiratory

A one-step multiplex real-time RT-PCR assay for rapid and simultaneous detection of human norovirus genogroup I, II and IV

2013 ◽  
Vol 189 (2) ◽  
pp. 277-282 ◽  
Author(s):  
Yong Yan ◽  
Heng-hui Wang ◽  
Lei Gao ◽  
Ji-mei Ji ◽  
Zhi-jie Ge ◽  
...  
Keyword(s):  
Real Time ◽  
Simultaneous Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Human Norovirus ◽  
One Step

Development of a one step real-time RT-PCR method for sensitive detection of human astrovirus

2006 ◽  
Vol 133 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Enrique Royuela ◽  
Ana Negredo ◽  
Alicia Sánchez-Fauquier
Keyword(s):  
Real Time ◽  
Sensitive Detection ◽  
Rt Pcr ◽  
One Step ◽  
Human Astrovirus ◽  
Pcr Method

Rapid and quantitative detection of mumps virus RNA by one-step real-time RT-PCR

2004 ◽  
Vol 49 (2) ◽  
pp. 83-88 ◽  
Author(s):  
Ayhan Kubar ◽  
Mehmet Yapar ◽  
Bulent Besirbellioglu ◽  
I.Yasar Avci ◽  
Cakır Guney
Keyword(s):  
Real Time ◽  
Mumps Virus ◽  
Quantitative Detection ◽  
Rt Pcr ◽  
Virus Rna ◽  
One Step

Cell culture demonstrates superior sensitivity over one step real time RT PCR and nested VP1 amplification for Enteroviruses

2021 ◽  
Vol 287 ◽  
pp. 113994
Author(s):  
Auwal Idris Kabuga ◽  
Ahmad Nejati ◽  
Parastoo Soheili ◽  
Soodeh Yousefipoor ◽  
Maryam Yousefi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Rt Pcr ◽  
One Step

Rapid detection of Enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay

2017 ◽  
Vol 33 ◽  
pp. 8-10 ◽  
Author(s):  
Jingfang Chen ◽  
Rusheng Zhang ◽  
Xinhua Ou ◽  
Dong Yao ◽  
Zheng Huang ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
One Step
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