Isolation of hepatitis E virus genotype 4 from patients with acute cryptogenic hepatitis in Korea

Isolates of hepatitis E virus (HEV) have recently been described from China that are distinct from Burmese, Mexican and US viruses and constitute a novel genotype (genotype 4). Here, the complete genomic sequence of a representative isolate of genotype 4 HEV, amplified directly from the stool of an acutely infected patient, is presented. Analysis of the entire sequence confirms our previous conclusion, based upon partial sequence data, that these Chinese isolates belong to a novel genotype. Typical of genetic variation in HEV, most nucleotide substitutions occur in the third base of the codon and do not affect the amino acid sequence. The genotype 4 virus is unusual in that a single nucleotide insertion in the ORF 3 region changes the initiation of ORF 3, and perhaps also ORF 2. The consequences of these changes are discussed.

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Characterization of antigenic epitopes of ORF2 encoded proteins of hepatitis E virus genotype 4 identified in China

Hereditas (Beijing) ◽

10.1360/yc-007-0637 ◽

2007 ◽

Vol 29 (05) ◽

pp. 637

Author(s):

Hong-Mei ZHANG

Keyword(s):

Hepatitis E Virus ◽

Hepatitis E ◽

Virus Genotype ◽

Genotype 4 ◽

Encoded Proteins ◽

Antigenic Epitopes

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First Isolation of Hepatitis E Virus Genotype 4 in Europe through Swine Surveillance in the Netherlands and Belgium

PLoS ONE ◽

10.1371/journal.pone.0022673 ◽

2011 ◽

Vol 6 (8) ◽

pp. e22673 ◽

Cited By ~ 93

Author(s):

Renate W. Hakze-van der Honing ◽

Els van Coillie ◽

Adriaan F. G. Antonis ◽

Wim H. M. van der Poel

Keyword(s):

The Netherlands ◽

Hepatitis E Virus ◽

Hepatitis E ◽

Virus Genotype ◽

Genotype 4

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Hepatitis E virus genotype 4 in a pig farm, Italy, 2013

Epidemiology and Infection ◽

10.1017/s0950268814001150 ◽

2014 ◽

Vol 143 (3) ◽

pp. 529-533 ◽

Cited By ~ 31

Author(s):

I. MONNE ◽

L. CEGLIE ◽

G. DI MARTINO ◽

A. NATALE ◽

S. ZAMPROGNA ◽

...

Keyword(s):

Hepatitis E Virus ◽

Hepatitis E ◽

Genotype 3 ◽

Virus Genotype ◽

Genotype 4 ◽

North East ◽

The North ◽

Pig Farm ◽

Swine Population ◽

First Time

SUMMARYZoonotic strains of hepatitis E virus (HEV) in Europe have been reported to belong to genotypes 3 and 4. In 2012 and 2013, 57 pig farms in Northern Italy that had previously resulted seropositive for HEV were surveyed for the presence of the virus, with positive samples subsequently genotyped. Hepatitis E RNA was identified in 17/57 (29·8%) seropositive farms. Phylogenetic analysis demonstrated that distinct subtypes of genotype 3 were circulating in the north-east of Italy; as well, for the first time in the Italian swine population, genotype 4 was identified and attributed to subtype d.

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