Single-cell immunopathology of systemic contact allergy associated with corticosteroids

Author(s):  
Rebecca J Hertzman ◽  
Pooja Deshpande ◽  
Katie D White ◽  
Rama Gangula ◽  
Abha Chopra ◽  
...  
Keyword(s):  
2021 ◽  
Vol 147 (2) ◽  
pp. AB31
Author(s):  
Rebecca Hertzman ◽  
Pooja Deshpande ◽  
Katie White ◽  
Rama Gangula ◽  
Abha Chopra ◽  
...  
Keyword(s):  

Author(s):  
Debby A. Jennings ◽  
Michael J. Morykwas ◽  
Louis C. Argenta

Grafts of cultured allogenic or autogenic keratlnocytes have proven to be an effective treatment of chronic wounds and burns. This study utilized a collagen substrate for keratinocyte and fibroblast attachment. The substrate provided mechanical stability and augmented graft manipulation onto the wound bed. Graft integrity was confirmed by light and transmission electron microscopy.Bovine Type I dermal collagen sheets (100 μm thick) were crosslinked with 254 nm UV light (13.5 Joules/cm2) to improve mechanical properties and reduce degradation. A single cell suspension of third passage neonatal foreskin fibroblasts were plated onto the collagen. Five days later, a single cell suspension of first passage neonatal foreskin keratinocytes were plated on the opposite side of the collagen. The grafts were cultured for one month.The grafts were fixed in phosphate buffered 4% formaldehyde/1% glutaraldehyde for 24 hours. Graft pieces were then washed in 0.13 M phosphate buffer, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Polybed 812.


1972 ◽  
Vol 106 (5) ◽  
pp. 715-716 ◽  
Author(s):  
G. A. Gellin
Keyword(s):  

Author(s):  
Alexander Lind ◽  
Falastin Salami ◽  
Anne‐Marie Landtblom ◽  
Lars Palm ◽  
Åke Lernmark ◽  
...  

2020 ◽  
Vol 26 (10) ◽  
pp. 1644-1653 ◽  
Author(s):  
Wanxin Wang ◽  
Felipe Vilella ◽  
Pilar Alama ◽  
Inmaculada Moreno ◽  
Marco Mignardi ◽  
...  

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