Kinetic modelling, production optimization, functional characterization and phyto-toxicity evaluation of biosurfactant derived from crude oil biodegrading Pseudomonas sp. IITISM 19

2022 ◽  
Vol 10 (2) ◽  
pp. 107190
Author(s):  
Madhurya Ray ◽  
Vipin Kumar ◽  
Chiranjib Banerjee
Author(s):  
Jiaqiang Jing ◽  
Hongbin Niu ◽  
Lingbin Pan ◽  
Xiaoqin Xiong ◽  
Liwen Tan

BZ25-1 oilfield is located in the southeast of Bohai bay which geographically lies between 119°00′ to 119°15′ east longitude and 38°10′ to 38°20′ north latitude. It has two oil blocks, including Shahejie (SHJ) waxy oil and Minghuazhen (MHZ) heavy oil, with six wellhead platforms WHPA∼WHPF and six submarine pipelines. Therein, the WHPC-WHPB and WHPB-SPM (Single Point Mooring) pipelines transport the mixture of the two produced crude oils. However, the mixing of the two oils will certainly bring out a change in their components and properties, which directly affects the safe operation of the submarine pipelines and offshore production facilities. Therefore, this paper compounds three kinds of MHZ/SHJ mixed oils with blending ratios of 1:1, 3:1 and 9:1, mainly studies how the components, rheological and thermophysical properties of the oil mixtures change with the blending ratio. The major objective of this study is to evaluate the compatibility of the two crude oils and provide a theoretical basis for the production optimization and risk elusion of the oilfield. The results of the study show that the components and properties of SHJ crude oil are quite different from those of MHZ oil, the flow behavior of SHJ oil is more sensitive to temperature. As MHZ oil in the compounds increases, the contents of asphaltene, resin, sulfur and carbon residue will increase except wax contents, their viscosities, densities and flash points will also increase, but their pour points, yield stresses, calorific values and other major thermophysical parameters will decrease. A blending ratio of 2∼7:1 for MHZ to SHJ crude oil can be concluded to make the properties of the compounds meet the safe and economic requirements of the subsea pipeline and offshore facility operations and ensure the compatibility of the mixed oils. In actuality, the field operations have confirmed that the recommended blending ratio is reasonable and practicable.


2016 ◽  
Vol 75 (5) ◽  
Author(s):  
Abdullah M. El Mahdi ◽  
Hamidi Abdul Aziz ◽  
Salem S. Abu Amr ◽  
Nour Sh El-Gendy ◽  
Hussein N. Nassar

2017 ◽  
Vol 23 (4) ◽  
pp. 37-44
Author(s):  
SYLVESTER UWADIAE ◽  
EMIKE OMOAYENA

This study was aimed at assessing the effectiveness of indigenous microbes for remediation of hydrocarbon contaminated soil by first increasing the population of the indigenous microbes via bioaugmentation. Soil samples were treated using a consortium of bacteria: Bacillus substilis and Pseudomonas sp. which were isolated and cultured from the contaminated soil. The non-bacteria injected soil sample had the highest hydrocarbon content (THC) of 271.021 in comparison with the other soil samples. The THC percentage removal of B1 (96.885 %), B2 (97.562 %), B3 (98.835 %), B4 (99.594 %) and B5 (99.540 %) were higher than that of the control; indicating that biodegradation actually took place.


2019 ◽  
Vol 6 (4) ◽  
pp. 106
Author(s):  
Wa Gao ◽  
Dongyang Li ◽  
Hong You

Chlorantraniliprole (CAP) is a widely used insecticide in many areas due to its excellent insecticidal ability and mammalian safety, however, the removal of CAP has not been extensively studied. In this study, a bacterial strain GW13, which is capable of co-metabolizing CAP, was isolated from a vegetable field soil. The strain was identified as Pseudomonas sp. based on its physico-biochemical characteristics and 16S rRNA gene analysis. The bacterial strain GW13 could degrade CAP through co-metabolism, and glucose was the best additional carbon resource. In the presence of 1.0 g/L glucose, GW13 could co-metabolize over 80% of 200 mg/L CAP in 24 h. The degradation rate increased after 6 h and slowed again after 10 h. The GW13 genome analysis revealed many genes associated with metabolism, showing the degradation mechanism of GW13 from the genomic perspective. The EAWAG-BBD (Swiss Federal Institute of Aquatic Science and Technology Biocatalysis/Biodegradation Database) prediction results showed that the main pathway for CAP degradation is amide hydrolysis, which is consistent with many genes associated with amidase in the GW13 genome. This study may facilitate research on CAP biodegradation mechanisms in the environment.


1995 ◽  
Vol 41 (4-5) ◽  
pp. 418-424 ◽  
Author(s):  
Kasthuri Venkateswaran ◽  
Toshihiro Hoaki ◽  
Misako Kato ◽  
Tadashi Maruyama

Sediment samples from Japanese coasts were screened for microorganisms able to degrade resin components of crude oil, and a mixed population that could degrade 35% of 5000 ppm resin in 15 days was obtained. This population also metabolized 50% of saturates and aromatics present in crude oil (5000 ppm) in 7 days. A Pseudomonas sp. isolated from the mixed population emulsified and degraded 30% of resins. This strain also degraded saturates and aromatics (30%) present in crude oil (5000 ppm). This is the first report describing organisms that are able to grow on the resin fraction of crude oil as a sole source of carbon and energy.Key words: resin, crude oil, biodegradation, Iatroscan.


2013 ◽  
Vol 29 (11) ◽  
pp. 2195-2206 ◽  
Author(s):  
Yun Gao ◽  
Yue Xi ◽  
Xiao-Ling Lu ◽  
Heng Zheng ◽  
Bo Hu ◽  
...  

2020 ◽  
Vol 14 (2) ◽  
pp. 1545-1556
Author(s):  
G Varatharaju ◽  
K Nithya ◽  
P. Suresh ◽  
M. Rekha ◽  
N Balasubramanian ◽  
...  

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