Antiviral activity of Isatidis Radix derived glucosinolate isomers and their breakdown products against influenza A in vitro/ovo and mechanism of action

The currently available drugs against influenza A virus primarily target neuraminidase (NA) or the matrix protein 2 (M2) ion channel. The emergence of drug-resistant viruses requires the development of new antiviral chemicals. Our study applied a cell-based approach to evaluate the antiviral activity of a series of newly synthesized benzoic acid derivatives, and 4-(2,2-Bis(hydroxymethyl)-5-oxopyrrolidin-l-yl)-3-(5-cyclohexyl-4H-1,2,4-triazol-3-yl)amino). benzoic acid, termed NC-5, was found to possess antiviral activity. NC-5 inhibited influenza A viruses A/FM/1/47 (H1N1), A/Beijing/32/92 (H3N2) and oseltamivir-resistant mutant A/FM/1/47-H275Y (H1N1-H275Y) in a dose-dependent manner. The 50% effective concentrations (EC50) for H1N1 and H1N1-H275Y were 33.6 μM and 32.8 μM, respectively, which showed that NC-5 had a great advantage over oseltamivir in drug-resistant virus infections. The 50% cytotoxic concentration (CC50) of NC-5 was greater than 640 μM. Orally administered NC-5 protected mice infected with H1N1 and H1N1-H275Y, conferring 80% and 60% survival at 100 mg/kg/d, reducing body weight loss, and alleviating virus-induced lung injury. NC-5 could suppress NP and M1 protein expression levels during the late stages of viral biosynthesis and inhibit NA activity, which may influence virus release. Our study proved that NC-5 has potent anti-influenza activity in vivo and in vitro, meaning that it could be regarded as a promising drug candidate to treat infection with influenza viruses, including oseltamivir-resistant viruses.

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#Nitrosocarbonyls 1: Antiviral Activity ofN-(4-Hydroxycyclohex-2-en-1-yl)quinoline-2-carboxamide against the Influenza A Virus H1N1

The Scientific World JOURNAL ◽

10.1155/2014/472373 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Dalya Al-Saad ◽

Misal Giuseppe Memeo ◽

Paolo Quadrelli

Keyword(s):

Influenza Virus ◽

Antiviral Activity ◽

Small Molecules ◽

Influenza A ◽

Nucleoside Analogues ◽

Virus H1n1 ◽

Antiviral Activities ◽

Synthetic Approaches ◽

Carbocyclic Nucleoside

Influenza virus flu A H1N1 still remains a target for its inhibition with small molecules. Fleeting nitrosocarbonyl intermediates are at work in a short-cut synthesis of carbocyclic nucleoside analogues. The strategy of the synthetic approaches is presented along with thein vitroantiviral tests. The nucleoside derivatives were tested for their inhibitory activity against a variety of viruses. Promising antiviral activities were found for specific compounds in the case of flu A H1N1.

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Erratum to: “In vitro Antiviral Activity of Recombinant Antibodies of IgG and IgA Isotypes to Hemagglutinin of the Influenza A Virus” [Molecular Biology51, 804 (2017)]

Molecular Biology ◽

10.1134/s0026893318090017 ◽

2018 ◽

Vol 52 (5) ◽

pp. 786-786

Author(s):

V. V. Argentova ◽

T. K. Aliev ◽

V. V. Zarubaev ◽

S. A. Klotchenko ◽

A. A. Shtro ◽

...

Keyword(s):

Antiviral Activity ◽

Influenza A Virus ◽

Influenza A ◽

Recombinant Antibodies

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Antiviral activity of Ouyi Antipyretic detoxicate soft capsule against influenza a virus H1N1 in vitro

China Journal of Chinese Materia Medica ◽

10.4268/cjcmm20111432 ◽

2011 ◽

Author(s):

HE Fenglan

Keyword(s):

Antiviral Activity ◽

Influenza A Virus ◽

Influenza A ◽

Virus H1n1

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Antiviral Activity of Arabinosylthymine in Herpesviral Replication: Mechanism of Action In Vivo and In Vitro

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.12.2.243 ◽

1977 ◽

Vol 12 (2) ◽

pp. 243-254 ◽

Cited By ~ 54

Author(s):

J. F. Aswell ◽

G. P. Allen ◽

A. T. Jamieson ◽

D. E. Campbell ◽

G. A. Gentry

Keyword(s):

Antiviral Activity ◽

Mechanism Of Action ◽

Replication Mechanism

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Antiviral activity of Chongkukjang extracts against influenza A virus in vitro and in vivo

Journal of Ethnic Foods ◽

10.1016/j.jef.2015.04.001 ◽

2015 ◽

Vol 2 (2) ◽

pp. 47-51 ◽

Cited By ~ 6

Author(s):

Bai Wei ◽

Se-Yeoun Cha ◽

Min Kang ◽

Young Jin Kim ◽

Chang-Won Cho ◽

...

Keyword(s):

Antiviral Activity ◽

Influenza A Virus ◽

Influenza A

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In Vitro Inhibition of Zika Virus Replication with Amantadine and Rimantadine Hydrochlorides

Microbiology Research ◽

10.3390/microbiolres12030052 ◽

2021 ◽

Vol 12 (3) ◽

pp. 727-738

Author(s):

Jorge L. Arias-Arias ◽

Francisco Vega-Aguilar ◽

Dihalá Picado-Soto ◽

Eugenia Corrales-Aguilar ◽

Gilbert D. Loría

Keyword(s):

Antiviral Activity ◽

Zika Virus ◽

Influenza A ◽

Yellow Fever Virus ◽

Human Infection ◽

Virus Infections ◽

Drug Concentrations ◽

Dose Dependent ◽

Reference Strains

Zika virus (ZIKV) is a mosquito-borne flavivirus in which human infection became relevant during recent outbreaks in Latin America due to its unrecognized association with fetal neurological disorders. Currently, there are no approved effective antivirals or vaccines for the treatment or prevention of ZIKV infections. Amantadine and rimantadine are approved antivirals used against susceptible influenza A virus infections that have been shown to have antiviral activity against other viruses, such as dengue virus (DENV). Here, we report the in vitro effectiveness of both amantadine and rimantadine hydrochlorides against ZIKV replication, resulting in a dose-dependent reduction in viral titers of a ZIKV clinical isolate and two different ZIKV reference strains. Additionally, we demonstrate similar in vitro antiviral activity of these drugs against DENV-1 and yellow fever virus (YFV), although at higher drug concentrations for the latter. ZIKV replication was inhibited at drug concentrations well below cytotoxic levels of both compounds, as denoted by the high selectivity indexes obtained with the tested strains. Further work is absolutely needed to determine the potential clinical use of these antivirals against ZIKV infections, but our results suggest the existence of a highly conserved mechanism across flavivirus, susceptible to be blocked by modified more specific adamantane compounds.

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In vitro evaluation of the antiviral effects of the homeopathic preparation Gripp-Heel on selected respiratory virusesThis article is one of a selection of papers published in this special issue (part 2 of 2) on the Safety and Efficacy of Natural Health Products.

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y07-100 ◽

2007 ◽

Vol 85 (11) ◽

pp. 1084-1090 ◽

Cited By ~ 8

Author(s):

Bernadette Glatthaar-Saalmüller

Keyword(s):

Antiviral Activity ◽

Influenza A ◽

Human Herpesvirus ◽

Mononuclear Leukocytes ◽

Type I ◽

Natural Health Products ◽

Peripheral Blood Mononuclear ◽

Syncytial Virus ◽

Homeopathic Preparation

Gripp-Heel® is a homeopathic preparation frequently used in the treatment of respiratory viral infections such as various types of influenza and the common cold. The antiviral activity of Gripp-Heel was studied in vitro on human pathogenic enveloped and nonenveloped RNA and DNA viruses. Before the antiviral assays, in vitro cytotoxicity of Gripp-Heel was determined with cells used for the infection experiments (HeLa, HEp-2, MDCK, BGM) as well as with mitogen-stimulated peripheral blood mononuclear leukocytes. A concentration of 0.5 of the commercially available product slightly reduced cell viability and proliferative capacity, and experiments on antiviral activity were determined starting with a dilution of 0.2 of the commercially available product. The antiviral activity was determined against a broad panel of enveloped and nonenveloped DNA and RNA viruses with plaque reduction assay, cytopathogenic assays, virus titrations, analysis of the viral proteins in virus-specific enzyme immunoassays, and haemagglutination tests. Control substances were acyclovir (10 μg/mL), ribavirin (6 μg/mL), and amantadine hydrochloride (5 μg/mL), depending on the virus type. Gripp-Heel demonstrated dose-dependent in vitro activity (significant reductions of infectivity by 20% to 40%) against Human herpesvirus 1, Human adenovirus C serotype 5, Influenza A virus, Human respiratory syncytial virus, Human parainfluenza virus 3, Human rhinovirus B serotype 14, and Human coxsackievirus serotype A9. The mechanisms of this antiviral activity are still unclear, but type I interferon induction might be a possible explanation. Further research on this homeopathic preparation seems warranted.

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