Characterization of Schinziophyton rautanenii (Manketti) nut oil from Namibia rich in conjugated fatty acids and tocopherol

A chemotaxonomic study was carried out on representative strains of 13 Aeromonas genomic species. Quinone, polyamine, and fatty acid patterns were found to be very useful for an improved characterization of the genus and an improved differentiation from members of the families Enterobacteriaceae and Vibrionaceae. The Q-8-benzoquinone was the predominant ubiquinone, and putrescine and diaminopropane were the major poly amines of the genus. The fatty acid patterns of 181 strains, all characterized by DNA–DNA hybridization, showed a great homogeneity within the genus, with major amounts of hexadecanoic acid (16:0), hexadecenoic acid (16:1), and octadecenoic acid (18:1), and minor amounts of the hydroxylated fatty acids (3-OH 13:0, 2-OH 14:0, 3-OH 14:0) in addition to some iso and anteiso branched fatty acids (i-13:0, i-17:1, i-17:0, and a-17:0). Although some differences in fatty acid profiles between the genomic species could be observed, a clearcut differentiation of all species was not possible.Key words: Aeromonas, polyamines, quinones, fatty acids, differentiation.

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Sponge Fatty Acids, 5. Characterization of Complete Series of 2-Hydroxy Long-Chain Fatty Acids in Phospholipids of Two Senegalese Marine Sponges from the Family Suberitidae: Pseudosuberites sp. and Suberites massa

Journal of Natural Products ◽

10.1021/np50102a012 ◽

1993 ◽

Vol 56 (12) ◽

pp. 2104-2113 ◽

Cited By ~ 23

Author(s):

Gilles Barnathan ◽

Jean-Michel Kornprobst ◽

Pierre Doumenq ◽

Joseph Miralles ◽

Nicole Boury-Esnault

Keyword(s):

Fatty Acids ◽

Marine Sponges ◽

Long Chain Fatty Acids ◽

The Family ◽

Complete Series ◽

Long Chain ◽

Chain Fatty Acids

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A survey of the conjugated fatty acids of seed oils

Journal of the American Oil Chemists Society ◽

10.1007/bf02915346 ◽

1968 ◽

Vol 45 (3) ◽

pp. 176-182 ◽

Cited By ~ 34

Author(s):

C. Y. Hopkins ◽

Mary J. Chisholm

Keyword(s):

Fatty Acids ◽

Seed Oils ◽

Conjugated Fatty Acids

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Separation and identification of isomeric conjugated fatty acids by high-performance liquid chromatography with photodiode array detection

Lipids ◽

10.1007/bf02537128 ◽

1993 ◽

Vol 28 (11) ◽

pp. 1037-1040 ◽

Cited By ~ 8

Author(s):

Sajid Husain ◽

K. Sita Devi

Keyword(s):

Fatty Acids ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Photodiode Array Detection ◽

Conjugated Fatty Acids ◽

Photodiode Array ◽

Array Detection

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Channeling of Eukaryotic Diacylglycerol into the Biosynthesis of Plastidial Phosphatidylglycerol

Journal of Biological Chemistry ◽

10.1074/jbc.m606295200 ◽

2006 ◽

Vol 282 (7) ◽

pp. 4613-4625 ◽

Cited By ~ 26

Author(s):

Markus Fritz ◽

Heiko Lokstein ◽

Dieter Hackenberg ◽

Ruth Welti ◽

Mary Roth ◽

...

Keyword(s):

Fatty Acids ◽

Double Bond ◽

Transgenic Plants ◽

Phosphatidic Acid ◽

Lipid Analysis ◽

Photosynthetic Apparatus ◽

Hexadecenoic Acid ◽

Transgenic Tobacco Plants ◽

Tobacco Plants

Plastidial glycolipids contain diacylglycerol (DAG) moieties, which are either synthesized in the plastids (prokaryotic lipids) or originate in the extraplastidial compartment (eukaryotic lipids) necessitating their transfer into plastids. In contrast, the only phospholipid in plastids, phosphatidylglycerol (PG), contains exclusively prokaryotic DAG backbones. PG contributes in several ways to the functions of chloroplasts, but it is not known to what extent its prokaryotic nature is required to fulfill these tasks. As a first step toward answering this question, we produced transgenic tobacco plants that contain eukaryotic PG in thylakoids. This was achieved by targeting a bacterial DAG kinase into chloroplasts in which the heterologous enzyme was also incorporated into the envelope fraction. From lipid analysis we conclude that the DAG kinase phosphorylated eukaryotic DAG forming phosphatidic acid, which was converted into PG. This resulted in PG with 2–3 times more eukaryotic than prokaryotic DAG backbones. In the newly formed PG the unique Δ3-trans-double bond, normally confined to 3-trans-hexadecenoic acid, was also found in sn-2-bound cis-unsaturated C18 fatty acids. In addition, a lipidomics technique allowed the characterization of phosphatidic acid, which is assumed to be derived from eukaryotic DAG precursors in the chloroplasts of the transgenic plants. The differences in lipid composition had only minor effects on measured functions of the photosynthetic apparatus, whereas the most obvious phenotype was a significant reduction in growth.

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