Ice-crystal formation in gelatin gel during pressure shift versus conventional freezing

2005 ◽  
Vol 66 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Songming Zhu ◽  
Hosahalli S. Ramaswamy ◽  
Alain Le Bail
Keyword(s):  
Crystal Formation ◽  
Ice Crystal ◽  
Pressure Shift ◽  
Ice Crystal Formation ◽  
Conventional Freezing

The “KIMWIPE” Effect in Ultra-Thin Cryosections

1985 ◽  
Vol 43 ◽  
pp. 458-459
Author(s):  
I. Taylor ◽  
P. Ingram ◽  
J.R. Sommer
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Muscle Fibers ◽  
Ice Crystals ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Thin Sections ◽  
Skeletal Muscle Fibers ◽  
Freeze Dried ◽  
Ice Crystal Formation

In studying quick-frozen single intact skeletal muscle fibers for structural and microchemical alterations that occur milliseconds, and fractions thereof, after electrical stimulation, we have developed a method to compare, directly, ice crystal formation in freeze-substituted thin sections adjacent to all, and beneath the last, freeze-dried cryosections. We have observed images in the cryosections that to our knowledge have not been published heretofore (Figs.1-4). The main features are that isolated, sometimes large regions of the sections appear hazy and have much less contrast than adjacent regions. Sometimes within the hazy regions there are smaller areas that appear crinkled and have much more contrast. We have also observed that while the hazy areas remain still, the regions of higher contrast visibly contract in the beam, often causing tears in the sections that are clearly not caused by ice crystals (Fig.3, arrows).

scholarly journals Effects of freshness on thawing drip and ice crystal formation in frozen spotted mackerel Scomber australasicus

2015 ◽  
Vol 81 (1) ◽  
pp. 124-129 ◽  
Author(s):  
KANAKO HASHIMOTO ◽  
TOKIFUSA KAWASHIMA ◽  
NOBUYUKI YOSHINO ◽  
TAKAAKI SHIRAI ◽  
AKIHIDE TAKIGUCHI
Keyword(s):  
Crystal Formation ◽  
Ice Crystal ◽  
Ice Crystal Formation

scholarly journals FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS

1972 ◽  
Vol 53 (1) ◽  
pp. 116-126 ◽  
Author(s):  
Helmut Plattner ◽  
Walter M. Fischer ◽  
Werner W. Schmitt ◽  
Luis Bachmann
Keyword(s):  
Cell Damage ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Unicellular Organisms ◽  
Freeze Etching ◽  
Ice Crystal Formation

The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed significantly from that obtained from glycerol-treated, freeze-etched cells. Furthermore, viability studies of spray-frozen Chlorella compared favorably with cells frozen by other methods.

Sign in / Sign up

Export Citation Format

Share Document