640 Single-cell transcriptomics of human pressure ulcers reveals MHCII expressing keratinocytes in patients with a worse healing outcome

Abstract At present, skin pressure ulcers are a common problem in the care of bedridden patients. Solving this problem usually involves turning the patient over regularly, which requires a lot of manpower and material resources. This paper designs a human body pressure recognition model, which can solve the problem of human pressure ulcers very well in combination with intelligent nursing beds. This paper collects the bone data of the human body by using the Kinect sensor, and then processes the collected data. The film pressure sensor is used to collect the pressure information of the human body, and the pressure information is matched with the bone data of the human body, so as to obtain the pressure of the corresponding part of the human body, and judge the current posture of the human body through the pressure information. When the pressure of the compression part of the human body reaches the threshold, the intelligent nursing bed automatically turns over to reduce the pressure of the compression part of the human body.

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Wound Fluids from Human Pressure Ulcers Contain Elevated Matrix Metalloproteinase Levels and Activity Compared to Surgical Wound Fluids

Journal of Investigative Dermatology ◽

10.1111/1523-1747.ep12365637 ◽

1996 ◽

Vol 107 (5) ◽

pp. 743-748 ◽

Cited By ~ 261

Author(s):

Dorne R. Yager ◽

Liang-Y. Zhang ◽

Hui-Xiu Liang ◽

Robert F. Diegelmann ◽

I. Kelman Cohen

Keyword(s):

Matrix Metalloproteinase ◽

Pressure Ulcers ◽

Surgical Wound ◽

Human Pressure

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Electron microscopy of keratinocytes cultured on a collagen substrate used as an allograft for the treatment of chronic wounds

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100130158 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1104-1105

Author(s):

Debby A. Jennings ◽

Michael J. Morykwas ◽

Louis C. Argenta

Keyword(s):

Electron Microscopy ◽

Cell Suspension ◽

Single Cell ◽

Chronic Wounds ◽

Mechanical Stability ◽

Uv Light ◽

Type I ◽

Single Cell Suspension ◽

Collagen Substrate ◽

Dermal Collagen

Grafts of cultured allogenic or autogenic keratlnocytes have proven to be an effective treatment of chronic wounds and burns. This study utilized a collagen substrate for keratinocyte and fibroblast attachment. The substrate provided mechanical stability and augmented graft manipulation onto the wound bed. Graft integrity was confirmed by light and transmission electron microscopy.Bovine Type I dermal collagen sheets (100 μm thick) were crosslinked with 254 nm UV light (13.5 Joules/cm2) to improve mechanical properties and reduce degradation. A single cell suspension of third passage neonatal foreskin fibroblasts were plated onto the collagen. Five days later, a single cell suspension of first passage neonatal foreskin keratinocytes were plated on the opposite side of the collagen. The grafts were cultured for one month.The grafts were fixed in phosphate buffered 4% formaldehyde/1% glutaraldehyde for 24 hours. Graft pieces were then washed in 0.13 M phosphate buffer, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Polybed 812.

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