Proliferation and osteogenic differentiation of human mesenchymal stem cells in PCL/silanated silica composite scaffolds for bone tissue regeneration

Bone tissue engineering has developed significantly in recent years as there has been increasing demand for bone substitutes due to trauma, cancer, arthritis, and infections. The scaffolds for bone regeneration need to be mechanically stable and have a 3D architecture with interconnected pores. With the advances in additive manufacturing technology, these requirements can be fulfilled by 3D printing scaffolds with controlled geometry and porosity using a low-cost multistep process. The scaffolds, however, must also be bioactive to promote the environment for the cells to regenerate into bone tissue. To determine if a low-cost 3D printing method for bespoke SiOC(N) porous structures can regenerate bone, these structures were tested for osteointegration potential by using human mesenchymal stem cells (hMSCs). This includes checking the general biocompatibilities under the osteogenic differentiation environment (cell proliferation and metabolism). Moreover, cell morphology was observed by confocal microscopy, and gene expressions on typical osteogenic markers at different stages for bone formation were determined by real-time PCR. The results of the study showed the pore size of the scaffolds had a significant impact on differentiation. A certain range of pore size could stimulate osteogenic differentiation, thus promoting bone regrowth and regeneration.

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Hydrostatic pressure in combination with topographical cues affects the fate of bone marrow-derived human mesenchymal stem cells for bone tissue regeneration

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.36267 ◽

2017 ◽

Vol 106 (3) ◽

pp. 629-640 ◽

Cited By ~ 6

Author(s):

Yvonne Reinwald ◽

Alicia J. El Haj

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Hydrostatic Pressure ◽

Bone Tissue ◽

Tissue Regeneration ◽

Human Mesenchymal Stem Cells ◽

Bone Tissue Regeneration ◽

Topographical Cues

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3D Printed SiOC(N) Ceramic Scaffolds for Bone Tissue Regeneration: Improved Osteogenic Differentiation of Human Bone Marrow‐Derived Mesenchymal Stem Cells

10.20944/preprints202111.0360.v1 ◽

2021 ◽

Author(s):

Yuejiao Yang ◽

Apoorv Kulkarni ◽

Gian Domenico Soraru ◽

Joshua M Pearce ◽

Antonella Motta

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

3D Printing ◽

Pore Size ◽

Osteogenic Differentiation ◽

Bone Tissue ◽

Low Cost ◽

Human Mesenchymal Stem Cells ◽

Bone Tissue Regeneration ◽

Gene Expressions

Bone tissue engineering has developed significantly in recent years as the increasing demand for bone substitutes due to trauma, cancer, arthritis, and infections. The scaffolds for bone regeneration need to be mechanically stable and have a 3D architecture with interconnected pores. With the advances in additive manufacturing technology, these requirements can be fulfilled by 3D printing scaffolds with controlled geometry and porosity using a low-cost multistep process. The scaffolds, however, must also be bioactive to promote the environment for the cells to regenerate into bone tissue. To determine if a low-cost 3D printing method for bespoke SiOC(N) porous structures can regenerate bone these structures were tested for osteointegration potential by using human mesenchymal stem cells (hMSCs). This includes checking the general biocompatibilities under the osteogenic differentiation environment (cell proliferation and metabolism). Moreover, cell morphology was observed by confocal microscopy and gene expressions on typical osteogenic markers at different stages for bone formation were determined by real-time PCR. The results of the study showed the pore size of the scaffolds had a significant impact on differentiation. A certain range of pore size could stimulate osteogenic differentiation, thus promoting bone regrowth and regeneration.

Download Full-text