Accurate quantitation for in vitro refolding of single domain antibody fragments expressed as inclusion bodies by referring the concomitant expression of a soluble form in the periplasms of Escherichia coli

2017 ◽  
Vol 442 ◽  
pp. 1-11 ◽  
Author(s):  
Tomoaki Noguchi ◽  
Yuichi Nishida ◽  
Keiji Takizawa ◽  
Yue Cui ◽  
Koki Tsutsumi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Antibody Fragments ◽  
Single Domain ◽  
Soluble Form ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
In Vitro Refolding

In vitro antiviral activity of single domain antibody fragments against poliovirus

2010 ◽  
Vol 87 (2) ◽  
pp. 257-264 ◽  
Author(s):  
Bert Thys ◽  
Lise Schotte ◽  
Serge Muyldermans ◽  
Ulrich Wernery ◽  
Gholamreza Hassanzadeh-Ghassabeh ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Antibody Fragments ◽  
Single Domain ◽  
Single Domain Antibody ◽  
Domain Antibody

Production, characterization and in-vitro applications of single-domain antibody against thyroglobulin selected from novel T7 phage display library

2021 ◽  
Vol 492 ◽  
pp. 112990
Author(s):  
Jothivel Kumarasamy ◽  
Samar Kumar Ghorui ◽  
Chandrakala Gholve ◽  
Bharti Jain ◽  
Yogesh Dhekale ◽  
...  
Keyword(s):  
Phage Display ◽  
Phage Display Library ◽  
Single Domain ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
T7 Phage Display ◽  
T7 Phage

scholarly journals A Single-Domain Antibody-Based Anti-PSMA Recombinant Immunotoxin Exhibits Specificity and Efficacy for Prostate Cancer Therapy

2021 ◽  
Vol 22 (11) ◽  
pp. 5501
Author(s):  
Yutong Xing ◽  
Keyuan Xu ◽  
Shixiong Li ◽  
Li Cao ◽  
Yue Nan ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Animal Studies ◽  
Single Domain ◽  
Soluble Form ◽  
Therapeutic Window ◽  
Single Domain Antibody ◽  
Pseudomonas Exotoxin A ◽  
Simple Strategy ◽  
Domain Antibody ◽  
Recombinant Immunotoxin

Prostate cancer (PCa) is the second most common cancer in men, causing more than 300,000 deaths every year worldwide. Due to their superior cell-killing ability and the relative simplicity of their preparation, immunotoxin molecules have great potential in the clinical treatment of cancer, and several such molecules have been approved for clinical application. In this study, we adopted a relatively simple strategy based on a single-domain antibody (sdAb) and an improved Pseudomonas exotoxin A (PE) toxin (PE24X7) to prepare a safer immunotoxin against prostate-specific membrane antigen (PSMA) for PCa treatment. The designed anti-PSMA immunotoxin, JVM-PE24X7, was conveniently prepared in its soluble form in an Escherichia coli (E. coli) system, avoiding the complex renaturation process needed for immunotoxin preparation by the conventional strategy. The product was very stable and showed a very strong ability to bind the PSMA receptor. Cytotoxicity assays showed that this molecule at a very low concentration could kill PSMA-positive PCa cells, with an EC50 value (concentration at which the cell viability decreased by 50%) of 15.3 pM against PSMA-positive LNCaP cells. Moreover, this molecule showed very good killing selectivity between PSMA-positive and PSMA-negative cells, with a selection ratio of more than 300-fold. Animal studies showed that this molecule at a very low dosage (5 × 0.5 mg/kg once every three days) completely inhibited the growth of PCa tumors, and the maximum tolerable dose (MTD) was more than 15 mg/kg, indicating its very potent tumor-treatment ability and a wide therapeutic window. Use of the new PE toxin, PE24X7, as the effector moiety significantly reduced off-target toxicity and improved the therapeutic window of the immunotoxin. The above results demonstrate that the designed anti-PSMA immunotoxin, JVM-PE24X7, has good application value for the treatment of PCa.

Over expression of anti-MUC1 single-domain antibody fragments in the yeast Pichia pastoris

2006 ◽  
Vol 43 (5) ◽  
pp. 426-435 ◽  
Author(s):  
Fatemeh Rahbarizadeh ◽  
Mohammad J. Rasaee ◽  
Mehdi Forouzandeh ◽  
Abdol-Amir Allameh
Keyword(s):  
Pichia Pastoris ◽  
Antibody Fragments ◽  
Single Domain ◽  
Single Domain Antibody ◽  
Yeast Pichia Pastoris ◽  
Over Expression ◽  
Domain Antibody

scholarly journals Improved Antitumor Efficacy of Chimeric Antigen Receptor T Cells that Secrete Single-Domain Antibody Fragments

2020 ◽  
Vol 8 (4) ◽  
pp. 518-529 ◽  
Author(s):  
Yushu Joy Xie ◽  
Michael Dougan ◽  
Jessica R. Ingram ◽  
Novalia Pishesha ◽  
Tao Fang ◽  
...  
Keyword(s):  
T Cells ◽  
Chimeric Antigen Receptor ◽  
Antigen Receptor ◽  
Antibody Fragments ◽  
Single Domain ◽  
Antitumor Efficacy ◽  
Single Domain Antibody ◽  
Domain Antibody

Passive immunization of guinea pigs with llama single-domain antibody fragments against foot-and-mouth disease

2007 ◽  
Vol 120 (3-4) ◽  
pp. 193-206 ◽  
Author(s):  
M.M. Harmsen ◽  
C.B. van Solt ◽  
H.P.D. Fijten ◽  
L. van Keulen ◽  
R.A. Rosalia ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Passive Immunization ◽  
Foot And Mouth Disease ◽  
Antibody Fragments ◽  
Mouth Disease ◽  
Single Domain ◽  
Single Domain Antibody ◽  
Domain Antibody ◽  
Foot And Mouth
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