Zero effect of Bt rice on expression of genes coding for digestion, detoxification and immune responses and developmental performances of Brown Planthopper Nilaparvata lugens (Stål)

2013 ◽  
Vol 59 (10) ◽  
pp. 985-993 ◽  
Author(s):  
Amani Mannakkara ◽  
Lin Niu ◽  
Weihua Ma ◽  
Chaoliang Lei
Keyword(s):  
Immune Responses ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Bt Rice ◽  
Expression Of Genes ◽  
Zero Effect ◽  
Nilaparvata Lugens Stål

scholarly journals Glucose Utilization in the Regulation of Chitin Synthesis in Brown Planthopper

2019 ◽  
Vol 19 (5) ◽  
Author(s):  
Bi-Ying Pan ◽  
Guo-Yong Li ◽  
Yan Wu ◽  
Zhong-Shi Zhou ◽  
Min Zhou ◽  
...  
Keyword(s):  
Metabolic Pathway ◽  
Glucose Utilization ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Chitin Synthesis ◽  
Expression Of Genes ◽  
Rnai Technology ◽  
Nilaparvata Lugens Stål ◽  
Synthesis And Degradation ◽  
Trehalose Content

Abstract Glucose-6-phosphatase (G6Pase) and hexokinase (HK) are two key enzymes in the glycolysis and gluconeogenesis pathways, which catalyze the synthesis and degradation of glucose in insects, respectively. G6Pase and HK play an important role in insect growth by regulating the metabolism of glucose, leading to the efficient metabolism of other macromolecules. However, it is unclear whether these genes could be investigated for pest control through their actions on chitin metabolism. We studied the potential functions of G6Pase and HK genes in the regulation of chitin metabolism pathways by RNAi technology. Interference with G6Pase expression did not affect trehalose and chitin metabolism pathways in brown planthopper, Nilaparvata lugens (Stål). However, knockdown of the HK gene resulted in a significant decrease of expression of genes associated with the trehalose metabolic pathway but had no significant effect on trehalase activity, trehalose content, or glucogen content. Additionally, HK knockdown resulting in downregulation of the genes involved in chitin metabolism in the brown planthopper. These insects also showed wing deformities and difficulty in molting to varying degrees. We suggest that the silencing of HK expression directly inhibited the decomposition of glucose, leading to impaired chitin synthesis.

scholarly journals Heat Stress Impedes Development and Lowers Fecundity of the Brown Planthopper Nilaparvata lugens (Stål)

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47413 ◽  
Author(s):  
Jiranan Piyaphongkul ◽  
Jeremy Pritchard ◽  
Jeff Bale
Keyword(s):  
Heat Stress ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Nilaparvata Lugens Stål

RNA interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

2015 ◽  
Vol 105 (4) ◽  
pp. 390-398 ◽  
Author(s):  
P.-J. Wan ◽  
L. Yang ◽  
S.-Y. Yuan ◽  
Y.-H. Tang ◽  
Q. Fu ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Transcript Level ◽  
Nilaparvata Lugens ◽  
The Body ◽  
Double Stranded Rna ◽  
Saccharopine Dehydrogenase ◽  
Body Weights ◽  
Nilaparvata Lugens Stål ◽  
Phloem Feeding ◽  
Wing Deformation

AbstractThe brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.

scholarly journals Fluktuasi Populasi Serangga Wereng Batang Coklat (Nilaparvata lugens) Pada Lahan Sawah Di Kabupaten Kerawang Jawa Barat

Agrologia ◽  
2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Martua Suhunan Sianipar
Keyword(s):  
Regression Analysis ◽  
Natural Enemy ◽  
Diversity Index ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Population Fluctuation ◽  
Plant Pests ◽  
Nilaparvata Lugens Stål ◽  
Experimental Plots

Brown Planthopper (Nilaparvata lugens Stal.) is a main pest of paddy. The purpose of this research is to study the population of Brown Planthopper and the natural enemy. The method of this research using survey. Survey was conducted in three experimental plots at JatisariSubdistrict, Karawang District, West Javaand in the greenhouse of the Department of Plant Pests and Deseases, Faculty of Agriculture,  Universitas Padjadjaran. The sample were collected by systematically diagonal using yellow stiky trap and manually or visual counting. The result showed population fluctuation BPH below the economy line where is temperature, humidity and rainfall did not significantly effect to the BPH population. It was indicated by the regression analysis of each temperature Y= (- 24.225 + 1.008 X1 ; R2 =0,020; P/Sign = 0.660),  Humidity  (Y=- 32.337 + 0.424 X2; R2  = 0,124; P/ Sign  = 0.261) and Rainfall (Y=1.707 + 0.090 X3 ; R2  = 0.050; P/ Sign = 0.487). There is a relation between temperature, humidity and rainfall to BPH (Y = - 123 + 2.685 T + 0.649 RH - 0.14 CH; R2 = 0.236; P/ Sign = 0.516) dit not significantly because as the value of significance (P = Sign)  > 0.05.8. The diversity index was moderate 1.88

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