Comparative studies on the invasion of cattle ticks (Rhipicephalus (Boophilus) microplus) and sheep blowflies (Lucilia cuprina) by Metarhizium anisopliae (Sorokin)

Cattle ticks,Rhipicephalus (Boophilus) microplus, are a serious threat to animal health and production. Some ticks feed on a single host species while others such asR. microplusinfest multiple hosts. White tailed deer (WTD) play a role in the maintenance and expansion of cattle tick populations. However, cattle ticks fed on WTD show lower weight and reproductive performance when compared to ticks fed on cattle, suggesting the existence of host factors that affect tick feeding and reproduction. To elucidate these factors, a proteomics approach was used to characterize tick and host proteins inR. microplusticks fed on cattle and WTD. The results showed thatR. microplusticks fed on cattle have overrepresented tick proteins involved in blood digestion and reproduction when compared to ticks fed on WTD, while host proteins were differentially represented in ticks fed on cattle or WTD. Although a direct connection cannot be made between differentially represented tick and host proteins, these results suggested that differentially represented host proteins together with other host factors could be associated with higherR. microplustick feeding and reproduction observed in ticks fed on cattle.

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Identification of potential plant extracts for anti-tick activity against acaricide resistant cattle ticks, Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)

Experimental and Applied Acarology ◽

10.1007/s10493-015-9890-7 ◽

2015 ◽

Vol 66 (1) ◽

pp. 159-171 ◽

Cited By ~ 21

Author(s):

Srikanta Ghosh ◽

Shashi Shankar Tiwari ◽

Bhanu Kumar ◽

Sharad Srivastava ◽

Anil Kumar Sharma ◽

...

Keyword(s):

Plant Extracts ◽

Boophilus Microplus ◽

Tick Activity ◽

Cattle Ticks

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In vitro activity of the hydroalcoholic extract of Chenopodium ambrosioides against engorged females of Rhipicephalus (Boophilus) microplus

Arquivos do Instituto Biológico ◽

10.1590/1808-1657000222016 ◽

2018 ◽

Vol 84 (0) ◽

Author(s):

Eduardo Oliveira ◽

Manoela da Silva ◽

Lew Sprenger ◽

Daniela Pedrassani

Keyword(s):

High Efficiency ◽

Boophilus Microplus ◽

Vitro Activity ◽

Economic Losses ◽

Cattle Tick ◽

Distilled Water ◽

In Vitro Activity ◽

Chenopodium Ambrosioides ◽

Cattle Ticks

ABSTRACT: Rhipicephalus (Boophilus) microplus, known as the cattle tick, is a cause of great economic losses for dairy cattle farming because of its high frequency of occurrence and the difficulty in controlling it. The aim of this study was to evaluate the in vitro activity of Chenopodium ambrosioides extract on R. (B.) microplus. For this purpose, 125 females were selected and classified into five groups according to their weight, in order to ensure that the females used presented homogeneous weight. The treatments comprised 40 and 60% extracts of Chenopodium ambrosioides, distilled water, ethanol (70ºGL) and 12.5% amitraz. The extracts of C. ambrosioides (40 and 60%) showed effectiveness of 99.7 and 100% and higher percentages of dead females than the other treatments: 64 and 96%, respectively (p<0.001). In the groups exposed to distilled water and ethanol (70º GL), 92 and 88% of the females maintained oviposition. In the females exposed to 40 and 60% extracts, oviposition of 36 and 4% occurred, respectively. It was concluded that the extract of Chenopodium ambrosioides, at both concentration evaluated, had high efficiency against engorged females of cattle ticks.

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Biological parameters of cattle ticks fed on rabbits

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612012000100005 ◽

2012 ◽

Vol 21 (1) ◽

pp. 22-27 ◽

Cited By ~ 2

Author(s):

Maria Alice Zacarias do Amaral ◽

Márcia Cristina de Azevedo Prata ◽

Erik Daemon ◽

John Furlong

Keyword(s):

Developmental Stages ◽

Experimental Studies ◽

Boophilus Microplus ◽

Cattle Tick ◽

Biological Parameters ◽

Dorsal Region ◽

Alternative Hosts ◽

Cattle Ticks ◽

Successive Generations

With the objective of encouraging the use of rabbits as alternative hosts for the cattle tick, four rabbits were infested on the ears and back. From the second day of infestation the developmental stages were observed. e duration of larval engorgement and changes were estimated between six and eight days in the region of the back and between 've and seven days in the pinna. e nymphal engorgement and changes occurred at approximately 10.80 ± 2.65 days in the dorsal and 11.00 ± 2.52 days in the ear, with the engorgement of adults and copulation occurring at 7.03 ± 2.45 days, on the dorsal region and 8.55 ± 1.82 days in the region of the pinna. e parasitic period ranged from 21 to 29 days (back) and from 23 to 30 days (pinna). e engorged females of the back and ear weighed on average 34.43 ± 18.73 and 36.30 ± 18.10 mg, respectively. e nutritional and reproductive e/ciency indexes were 17.38 ± 14.27 and 26.85 ± 17.13% (back) and 17.42 ± 12.22% and 30.70 ± 19.80% (pinna). Although not appropriate to maintain successive generations of Rhipicephalus (Boophilus) microplus, rabbits can be used for di:erent stages of engorgement of the ixodid, allowing experimental studies.

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Laboratory evaluation of virulence of Chinese Beauveria bassiana and Metarhizium anisopliae isolates to engorged female Rhipicephalus (Boophilus) microplus ticks

Biological Control ◽

10.1016/j.biocontrol.2012.07.002 ◽

2012 ◽

Vol 63 (2) ◽

pp. 98-101 ◽

Cited By ~ 11

Author(s):

Qiaoyun Ren ◽

Zhijie Liu ◽

Guiquan Guan ◽

Ming Sun ◽

Miling Ma ◽

...

Keyword(s):

Beauveria Bassiana ◽

Metarhizium Anisopliae ◽

Boophilus Microplus ◽

Laboratory Evaluation ◽

Boophilus Microplus Ticks

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Controle de larvas de Boophilus microplus por Metarhizium anisopliae em pastagens infestadas artificialmente

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2005000600010 ◽

2005 ◽

Vol 40 (6) ◽

pp. 595-600 ◽

Cited By ~ 11

Author(s):

Lúcia Mara de Souza Basso ◽

Antonio Carlos Monteiro ◽

Marco Antonio de Andrade Belo ◽

Vando Edesio Soares ◽

Marcos Valério Garcia ◽

...

Keyword(s):

Metarhizium Anisopliae ◽

Boophilus Microplus ◽

Brachiaria Brizantha

O objetivo deste trabalho foi avaliar a eficiência do controle exercido por Metarhizium anisopliae na população de Boophilus microplus, em pastagens de Brachiaria brizantha, e do híbrido Tifton 85 (Cynodon spp.), artificialmente infestadas com fêmeas ingurgitadas do carrapato. Trinta canteiros com 1 m² de área cada foram distribuídos aleatoriamente. Quinze foram pulverizados com esporos do fungo e quinze controles em cada forrageira, constituindo cinco repetições de cada tratamento, foram infestados com número e peso padronizados de fêmeas ingurgitadas do ácaro. Aplicou-se o fungo, na concentração de 1,8x10(8) conídios mL-1, em três situações: pulverização antes da infestação com o carrapato, após a infestação e posterioriormente à emergência das primeiras larvas nos capins. A ação do fungo foi avaliada no 35º, 38º, 41º, 48º, 55º e 61º dia pós-infestação, por meio da contagem de larvas recuperadas. Obteve-se controle de larvas do ácaro, que, nas avaliações realizadas entre o 35º e o 48º dia pós-infestação, variou entre 87% e 94%. As médias das contagens de estágios larvares do carrapato foram menores em todas as amostragens realizadas no capim-Tifton 85, indicando que houve efeito da pastagem na ação do fungo. A situação de aplicação influencia a atividade do fungo, com melhor resultado nas coletas realizadas entre o 41º e 55º dia após infestação em B. brizantha, e aplicação dos conídios logo após a emergência das primeiras larvas.

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Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612011000200003 ◽

2011 ◽

Vol 20 (2) ◽

pp. 103-110 ◽

Cited By ~ 4

Author(s):

Rodrigo Casquero Cunha ◽

Renato Andreotti ◽

Fábio Pereira Leivas Leite

Keyword(s):

Pichia Pastoris ◽

Boophilus Microplus ◽

Economic Losses ◽

Cattle Tick ◽

High Antibody ◽

Western Blot Assay ◽

Development Of Resistance ◽

Cattle Ticks ◽

Antibody Titers

The cattle tick Rhipicephalus (Boophilus) microplus is responsible for great economic losses. It is mainly controlled chemically, with limitations regarding development of resistance to the chemicals. Vaccines may help control this parasite, thereby reducing tick pesticide use. In this light, we performed subcloning of the gene of the protein Bm86-GC, the homologue protein that currently forms the basis of vaccines (GavacTM and TickGardPLUS) that have been developed against cattle ticks. The subcloning was done in the pPIC9 expression vector, for transformation in the yeast Pichia pastoris. This protein was characterized by expression of the recombinant Mut+ strain, which expressed greater quantities of protein. The expressed protein (rBm86-CG) was recognized in the Western-blot assay using anti-Gavac, anti-TickGard, anti-larval extract and anti-rBm86-CG polyclonal sera. The serum produced in cattle vaccinated with the antigen CG rBm86 presented high antibody titers and recognized the native protein. The rBm86-GC has potential relevance as an immunogen for vaccine formulation against cattle ticks.

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