Molecular characterization of an intrinsically disordered chaperone reveals net-charge regulation in chaperone action

2021 ◽  
pp. 167405
Author(s):  
Chang Ren ◽  
Yongxin Zheng ◽  
Chunlan Liu ◽  
Jun Mencius ◽  
Zhili Wu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Net Charge ◽  
Charge Regulation ◽  
Intrinsically Disordered

scholarly journals Molecular characterization of myelin basic protein a (mbpa) gene from red-bellied pacu (Piaractus brachypomus)

2022 ◽  
Vol 20 (1) ◽  
Author(s):  
Juan Sebastian Cruz-Méndez ◽  
María Paula Herrera-Sánchez ◽  
Ángel Enrique Céspedes-Rubio ◽  
Iang Schroniltgen Rondón-Barragán
Keyword(s):  
Gene Expression ◽  
Brain Injury ◽  
Myelin Basic Protein ◽  
Molecular Characterization ◽  
Basic Protein ◽  
Protein A ◽  
Intrinsically Disordered ◽  
Intrinsically Disordered Regions ◽  
Disordered Regions

Abstract Background Myelin basic protein (MBP) is one of the most important structural components of the myelin sheaths in both central and peripheral nervous systems. MBP has several functions including organization of the myelin membranes, reorganization of the cytoskeleton during the myelination process, and interaction with the SH3 domain in signaling pathways. Likewise, MBP has been proposed as a marker of demyelination in traumatic brain injury and chemical exposure. Methods The aim of this study was to molecularly characterize the myelin basic protein a (mbpa) gene from the Colombian native fish, red-bellied pacu, Piaractus brachypomus. Bioinformatic tools were used to identify the phylogenetic relationships, physicochemical characteristics, exons, intrinsically disordered regions, and conserved domains of the protein. Gene expression was assessed by qPCR in three models corresponding to sublethal chlorpyrifos exposure, acute brain injury, and anesthesia experiments. Results mbpa complete open reading frame was identified with 414 nucleotides distributed in 7 exons that encode 137 amino acids. MBPa was recognized as belonging to the myelin basic protein family, closely related with orthologous proteins, and two intrinsically disordered regions were established within the sequence. Gene expression of mbpa was upregulated in the optic chiasm of the chlorpyrifos exposed fish in contrast to the control group. Conclusions The physicochemical computed features agree with the biological functions of MBP, and basal gene expression was according to the anatomical distribution in the tissues analyzed. This study is the first molecular characterization of mbpa from the native species Piaractus brachypomus.

Biochemical and Molecular Characterization of a Novel Cu/Zn Superoxide Dismutase from Amaranthus hypochondriacus L.: an Intrinsically Disordered Protein

2015 ◽  
Vol 176 (8) ◽  
pp. 2328-2345 ◽  
Author(s):  
Gabriela M. Montero-Morán ◽  
José G. Sampedro ◽  
Gloria Saab-Rincón ◽  
Miguel A. Cervantes-González ◽  
José Á. Huerta-Ocampo ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Molecular Characterization ◽  
Intrinsically Disordered Protein ◽  
Amaranthus Hypochondriacus ◽  
Intrinsically Disordered ◽  
Disordered Protein

1446: Molecular Characterization of Cyclooxygenase-2 in a Stimulated Prostate Stroma Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom
Keyword(s):  
Molecular Characterization ◽  
Cyclooxygenase 2 ◽  
Prostate Stroma

Molecular characterization of lincRNAs in Down syndrome associated leukemia

2012 ◽  
Vol 224 (03) ◽  
Author(s):  
A Streltsov ◽  
S Emmrich ◽  
F Engeland ◽  
JH Klusmann
Keyword(s):  
Down Syndrome ◽  
Molecular Characterization

Molecular Characterization of Radiation-induced Glioblastoma

2018 ◽  
Author(s):  
MY Deng ◽  
D Sturm ◽  
E Pfaff ◽  
GP Balasubrama ◽  
J Schittenhelm ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Radiation Induced

Clinical and molecular characterization of patients with multiple sulfatase deficiency

2006 ◽  
Vol 37 (06) ◽  
Author(s):  
L Schlotawa ◽  
T Dierks ◽  
K von Figura ◽  
J Gärtner
Keyword(s):  
Molecular Characterization ◽  
Multiple Sulfatase Deficiency
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