Detection of respiratory viruses in adults with respiratory tract infection using a multiplex PCR assay at a tertiary center

Respiratory viruses are a common cause of respiratory tract infection (RTI), particularly in neonates and children. Rapid and accurate diagnosis of viral infections could improve clinical outcomes and reduce the use of antibiotics and treatment sessions. Advances in diagnostic technology contribute to the accurate detection of viruses. We performed a multiplex real-time polymerase chain reaction (PCR) to investigate the viral etiology in pediatric patients and compared the detection rates with those determined using traditional antigen tests and virus cultures. Fifteen respiratory viruses were included in our investigation: respiratory syncytial virus A/B (RSV), influenza virus A (FluA) and influenza virus B (FluB), human metapneumovirus (MPV), enterovirus (EV), human parainfluenza virus (PIV) types 1–4, human rhinovirus (RV), human coronavirus OC43, NL63, and 229E, human adenovirus (ADV), and human bocavirus (Boca). In total, 474 specimens were collected and tested. Respiratory viruses were detected more frequently by PCR (357, 75.3%) than they were by traditional tests (229, 49.3%). The leading pathogens were RSV (113, 23.8%), RV (72, 15.2%), PIV3 (53, 11.2%), FluA (51, 10.8%), and ADV (48, 10.1%). For children younger than 5 years, RSV and RV were most prevalent; for children older than 5 years, FluA and ADV were the most frequently detected. Of the specimens, 25.8% (92/357) were coinfected with two or more viruses. RV, Boca, PIV2, FluB, and PIV4 had higher rates of coinfection; MPV and PIV1 had the lowest rates of coinfection (9.1% and 5.3%). To conclude, the detection power of PCR was better than that of traditional antigen tests and virus cultures when considering the detection of respiratory viruses. RSV and RV were the leading viral pathogens identified in the respiratory specimens. One-quarter of the positive specimens were coinfected with two or more viruses. In the future, further application of PCR may contribute to the rapid and accurate diagnosis of respiratory viruses and could improve patient outcomes.

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Detection and Analysis of Respiratory Pathogens in Hospitalized Children with Acute Respiratory Tract Infections by a Multiplex-PCR Assay Based on the Genetic Analyzer Platform

Iranian Journal of Pediatrics ◽

10.5812/ijp.110203 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Pengbo Guo ◽

Yanhong Wang ◽

Mingjin Zhou ◽

Shiyue Mei ◽

Yibing Cheng

Keyword(s):

Respiratory Tract ◽

Multiplex Pcr ◽

Respiratory Tract Infections ◽

Hospitalized Children ◽

Respiratory Pathogens ◽

Pcr Assay ◽

Multiplex Pcr Assay ◽

Positive Rate ◽

Tract Infections ◽

Genetic Analyzer

Background: Despite the considerable incidence of acute respiratory tract infections (ARTI) among children in Henan province, detailed epidemiological information is limited Objectives: . Following a retrospective design, the current study aimed to analyze the epidemiological trends of respiratory pathogens in children hospitalized at Henan Children’s Hospital. Methods: A total of 11306 children (age range, 4 days to 15 years) diagnosed with ARTI admitted to Henan Children’s Hospital from March 1, 2019, to February 29, 2020, were enrolled. Nasopharyngeal swabs, alveolar lavage fluid, or sputum samples were analyzed for the presence of 12 pathogens via a multiplex-PCR assay based on the Genetic Analyzer platform. Data of 11306 samples were eligible for analysis. Results: The total positive rate was 78.1% (8831/11306). Of 8831 positive samples, 7017 (79.5%, 7017/8831) had a single pathogen and 1814 (20.5%, 1814/11306) had multiple pathogens. Human rhinovirus was the most common pathogen (25.4%, 2874/11306), followed by mycoplasma (18.1%, 2050/11306) and human respiratory syncytial virus (15.8%, 1783/11306). There was no significant difference concerning the positive rate of respiratory pathogens between boys and girls (χ2 = 0.286, P = 0.593). Children were more likely to be infected in autumn and winter than in spring and summer (9722 vs. 1584, respectively). Conclusions: Human rhinovirus and mycoplasma were the most commonly detected pathogens. The positive rate of chlamydia was independent of the season, while positive rates of other pathogens were season-related. The positive rate of influenza A (H1N1) was independent of age, while for other pathogens, it was age-dependent. This study demonstrated species-level information on the pathogens, which can improve the prevention and treatment of hospitalized children with ARTI.

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Multiplex PCR in Respiratory Tract Infection diagnosis

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v9i1.31313 ◽

2017 ◽

Vol 9 (1) ◽

pp. 1-2

Author(s):

Ahmed Abu Saleh

Keyword(s):

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Multiplex Pcr ◽

Infection Diagnosis

Bangladesh J Med Microbiol 2015; 09 (01): 01-02

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No impact of early real-time PCR screening for respiratory viruses on length of stay and use of antibiotics in elderly patients hospitalized with symptoms of a respiratory tract infection in a single center in Norway

European Journal of Clinical Microbiology & Infectious Diseases ◽

10.1007/s10096-013-1963-0 ◽

2013 ◽

Vol 33 (3) ◽

pp. 359-364 ◽

Cited By ~ 16

Author(s):

S. S. Hernes ◽

E. Hagen ◽

H. Quarsten ◽

B. Bjorvatn ◽

P. S. Bakke

Keyword(s):

Length Of Stay ◽

Tract Infection ◽

Respiratory Tract ◽

Elderly Patients ◽

Respiratory Tract Infection ◽

Real Time ◽

Real Time Pcr ◽

Respiratory Viruses ◽

Single Center ◽

Pcr Screening

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Frequency of respiratory viruses in children with lower respiratory tract infection

Türk Pediatri Arşivi ◽

10.4274/tpa.493 ◽

2013 ◽

Vol 48 (3) ◽

pp. 215-220 ◽

Cited By ~ 2

Author(s):

Sinem Akcali ◽

Nisel Yilmaz ◽

Ozlem Guler ◽

Tamer Sanildag ◽

Murat Anil

Keyword(s):

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Lower Respiratory Tract Infection ◽

Lower Respiratory Tract ◽

Respiratory Viruses

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Respiratory Virus Infections in Adults with Hematologic Malignancies: A Prospective Study

Clinical Infectious Diseases ◽

10.1086/344899 ◽

2003 ◽

Vol 36 (1) ◽

pp. 1-8 ◽

Cited By ~ 52

Author(s):

Rodrigo Martino ◽

Elena Rámila ◽

Núria Rabella ◽

José Manuel Muñoz ◽

Mercé Peyret ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Respiratory Virus ◽

Hematologic Malignancies ◽

Respiratory Viruses ◽

Virus Infections ◽

Syncytial Virus ◽

Respiratory Virus Infections

Abstract During a 2-year period, 157 consecutive episodes of respiratory virus infections that occurred in 130 patients with upper or lower respiratory tract infection were analyzed for respiratory viruses. A respiratory virus was identified in 75 episodes (48%), and several viruses were found in 13 episodes: there were a total of 56 influenza A virus infections, 14 respiratory syncytial virus infections, 8 adenovirus infections, 8 infections with parainfluenza virus types 1 or 3, and 7 enterovirus infections. On multivariate analysis, the only variable that predicted progression to pneumonia in patients with an upper respiratory tract infection was the presence of respiratory syncytial virus, whereas lymphocytopenia had a nonsignificant trend. Also, among the 38 patients who had pneumonia at any time during the episode, both respiratory syncytial virus and lymphocytopenia were commonly found. For both epidemiological and therapeutic considerations, frequent screening for respiratory viruses should be incorporated into the routine diagnostic study of patients with hematologic malignancies.

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