scholarly journals NMR spectroscopy of a single mammalian early stage embryo

2021 ◽  
pp. 107142
Author(s):  
Giulia Sivelli ◽  
Gaurasundar M. Conley ◽  
Carolina Herrera ◽  
Kathryn Marable ◽  
Kyle J. Rodriguez ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Early Stage ◽  
Stage Embryo ◽  
Early Stage Embryo

scholarly journals Fast Multi-Focus Fusion Based on Deep Learning for Early-Stage Embryo Image Enhancement

Sensors ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 863
Author(s):  
Vidas Raudonis ◽  
Agne Paulauskaite-Taraseviciene ◽  
Kristina Sutiene
Keyword(s):  
Deep Learning ◽  
Image Fusion ◽  
Early Stage ◽  
Image Data ◽  
Cell Detection ◽  
Processing Times ◽  
Fused Image ◽  
Stage Embryo ◽  
Early Stage Embryo

Background: Cell detection and counting is of essential importance in evaluating the quality of early-stage embryo. Full automation of this process remains a challenging task due to different cell size, shape, the presence of incomplete cell boundaries, partially or fully overlapping cells. Moreover, the algorithm to be developed should process a large number of image data of different quality in a reasonable amount of time. Methods: Multi-focus image fusion approach based on deep learning U-Net architecture is proposed in the paper, which allows reducing the amount of data up to 7 times without losing spectral information required for embryo enhancement in the microscopic image. Results: The experiment includes the visual and quantitative analysis by estimating the image similarity metrics and processing times, which is compared to the results achieved by two wellknown techniques—Inverse Laplacian Pyramid Transform and Enhanced Correlation Coefficient Maximization. Conclusion: Comparatively, the image fusion time is substantially improved for different image resolutions, whilst ensuring the high quality of the fused image.

scholarly journals Anticentromere antibody induced by immunization with centromere protein and Freund’s complete adjuvant may interfere with mouse early-stage embryo

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hanyan Liu ◽  
Yufen Zhang ◽  
Haiying Liu ◽  
Qing Huang ◽  
Ying Ying
Keyword(s):  
Early Stage ◽  
Protein A ◽  
Immunofluorescence Assay ◽  
Immunofluorescence Test ◽  
Anticentromere Antibody ◽  
Indirect Immunofluorescence Test ◽  
Centromere Protein ◽  
Centromere Protein A ◽  
Stage Embryo ◽  
Early Stage Embryo

Abstract Background Anticentromere antibody (ACA) is a member of the antinuclear antibody spectrum (ANAs) which has been speculated to be associated with subfertility. Thus, the present study aimed to investigate the induction of ACA production and its potential interference with early-stage embryos. Methods Recombinant centromere protein-A (CENP-A) or centromere protein-B (CENP-B) and complete Freund’s adjuvant (CFA) were used to immunize mice. Serum ACA level was then evaluated by using an indirect immunofluorescence test. Immunofluorescence assay was performed to detect IgG in follicles in ovarian tissues and early-stage embryos. Results Following treatment, serum positive ACA was observed in mice treated with CENP and CFA. Furthermore, IgG were detected in follicular fluid and early-stage embryos from mice treated with CENP and CFA. Conclusions This study preliminarily indicated that ACA induced by CENP and CFA may penetrate into the living embryos of early-stage in mice.

l -Carnitine affects the early stage embryo development

2017 ◽  
Vol 122 ◽  
pp. 37
Author(s):  
C. Kyvelidou ◽  
I. Athanassakis
Keyword(s):  
Embryo Development ◽  
Early Stage ◽  
Stage Embryo ◽  
Early Stage Embryo

Novel approach of scaffold-free tissue using human endometrial stromal cells to facilitate growth of early stage embryo in vitro

2017 ◽  
Vol 2017 (0) ◽  
pp. S0210103
Author(s):  
Jeonghyun KIM ◽  
Takayuki HARADA ◽  
Yasushi HIROTA ◽  
Takehiro HIRAOKA ◽  
Osamu YOSHINO ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Early Stage ◽  
Endometrial Stromal Cells ◽  
Novel Approach ◽  
Stage Embryo ◽  
Early Stage Embryo
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