Novel approach of scaffold-free tissue using human endometrial stromal cells to facilitate growth of early stage embryo in vitro

High level of uric acid (UA) is the major origin of gout, and is highly associated with various pregnant complications, such as preeclampsia and gestational diabetes. However, UA’s level and role in the very early stage of pregnancy has not been uncovered. This study aims to investigate the relevance of serum UA and decidualization, an essential process for the establishment and maintenance of pregnancy in women and mice during the early stage of pregnancy. In this study, we first proved that expression level of UA synthase xanthine dehydrogenase (XDH) is highly increased along with decidualization of endometrial stromal cells in both in vitro and in vivo models. Furthermore, serum and endometrial levels of UA are higher in mice with decidualized uterin horn and in vitro decidualized stromal cells. The existence of monosodium urate (MSU) crystal was also confirmed by immunostaining. Next, the roles of MSU on decidualization were explored by both in vitro and in vivo models. Our data shows MSU crystal but not UA enhances the decidualization response of endometrial stromal cells, via the upregulation of inflammatory genes such Ptgs2 and Il11. inhibiting of Cox-2 activity abolishes MSU crystal induced higher expression of decidualization marker Prl8a2. At last, in women, we observed enriched expression of XDH in decidua compare to non-decidualized endometrium, the serum level of UA is significantly increased in women in very early stage of pregnancy, and drop down after elective abortion. In summary, we observed an increased serum UA level in the early stage of women’s pregnancy, and proved that the increased level of UA results from the expressed XDH in decidualizing endometrium of both human and mouse, leading to the formation of MSU crystal. MSU crystal can enhance the decidualization response via inflammatory pathways. Our study has uncovered the association between UA, MSU, and decidualization during the early stage of pregnancy.

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Effect of flax seed (Linum usitatissimum) extract on modulation of prostaglandin E2 production by buffalo endometrial stromal cells cultured in vitro

Reproduction Abstracts ◽

10.1530/repabs.1.p307 ◽

2014 ◽

Author(s):

Singh Sanjay Kumar ◽

Chethan G Sharma ◽

Jessiehun Nongsiej ◽

R P Singh ◽

Agarwal Sudhir Kumar

Keyword(s):

Prostaglandin E2 ◽

Stromal Cells ◽

Linum Usitatissimum ◽

Flax Seed ◽

Endometrial Stromal Cells ◽

Cells Cultured

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Glucocorticoids trigger macrophage migration inhibitory factor (MIF) secretion by decidualized human endometrial stromal cells in vitro: the modulatory effect of Bisphenol A

Reproduction Abstracts ◽

10.1530/repabs.1.p276 ◽

2014 ◽

Author(s):

Chiara Mannelli ◽

Anna Szostek ◽

Sara Ciaffarafa ◽

Claudiopietro Carotenuto ◽

Francesca Ietta ◽

...

Keyword(s):

Bisphenol A ◽

Migration Inhibitory Factor ◽

Stromal Cells ◽

Macrophage Migration Inhibitory Factor ◽

Inhibitory Factor ◽

Macrophage Migration ◽

Endometrial Stromal Cells

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Fast Multi-Focus Fusion Based on Deep Learning for Early-Stage Embryo Image Enhancement

Sensors ◽

10.3390/s21030863 ◽

2021 ◽

Vol 21 (3) ◽

pp. 863

Author(s):

Vidas Raudonis ◽

Agne Paulauskaite-Taraseviciene ◽

Kristina Sutiene

Keyword(s):

Deep Learning ◽

Image Fusion ◽

Early Stage ◽

Image Data ◽

Cell Detection ◽

Processing Times ◽

Fused Image ◽

Stage Embryo ◽

Early Stage Embryo

Background: Cell detection and counting is of essential importance in evaluating the quality of early-stage embryo. Full automation of this process remains a challenging task due to different cell size, shape, the presence of incomplete cell boundaries, partially or fully overlapping cells. Moreover, the algorithm to be developed should process a large number of image data of different quality in a reasonable amount of time. Methods: Multi-focus image fusion approach based on deep learning U-Net architecture is proposed in the paper, which allows reducing the amount of data up to 7 times without losing spectral information required for embryo enhancement in the microscopic image. Results: The experiment includes the visual and quantitative analysis by estimating the image similarity metrics and processing times, which is compared to the results achieved by two wellknown techniques—Inverse Laplacian Pyramid Transform and Enhanced Correlation Coefficient Maximization. Conclusion: Comparatively, the image fusion time is substantially improved for different image resolutions, whilst ensuring the high quality of the fused image.

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Insulin-like growth factor-binding protein-1 is phosphorylated by cultured human endometrial stromal cells and multiple protein kinases in vitro.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)55239-x ◽

1991 ◽

Vol 266 (27) ◽

pp. 18082-18088

Author(s):

R.A. Frost ◽

L. Tseng

Keyword(s):

Growth Factor ◽

Protein Kinases ◽

Stromal Cells ◽

Binding Protein ◽

Insulin Like Growth Factor ◽

Endometrial Stromal Cells ◽

Factor Binding ◽

Multiple Protein

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Endometrial regeneration with endometrial epithelium: homologous orchestration with endometrial stroma as a feeder

Stem Cell Research & Therapy ◽

10.1186/s13287-021-02188-x ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Ryo Yokomizo ◽

Yukiko Fujiki ◽

Harue Kishigami ◽

Hiroshi Kishi ◽

Tohru Kiyono ◽

...

Keyword(s):

Epithelial Cells ◽

Stromal Cells ◽

Therapeutic Option ◽

Three Dimensional ◽

Fertility Treatment ◽

Feeder Cells ◽

Endometrial Stromal Cells ◽

Thin Endometrium ◽

Endometrial Epithelial Cells

Abstract Background Thin endometrium adversely affects reproductive success rates with fertility treatment. Autologous transplantation of exogenously prepared endometrium can be a promising therapeutic option for thin endometrium; however, endometrial epithelial cells have limited expansion potential, which needs to be overcome in order to make regenerative medicine a therapeutic strategy for refractory thin endometrium. Here, we aimed to perform long-term culture of endometrial epithelial cells in vitro. Methods We prepared primary human endometrial epithelial cells and endometrial stromal cells and investigated whether endometrial stromal cells and human embryonic stem cell-derived feeder cells could support proliferation of endometrial epithelial cells. We also investigated whether three-dimensional culture can be achieved using thawed endometrial epithelial cells and endometrial stromal cells. Results Co-cultivation with the feeder cells dramatically increased the proliferation rate of the endometrial epithelial cells. We serially passaged the endometrial epithelial cells on mouse embryonic fibroblasts up to passage 6 for 4 months. Among the human-derived feeder cells, endometrial stromal cells exhibited the best feeder activity for proliferation of the endometrial epithelial cells. We continued to propagate the endometrial epithelial cells on endometrial stromal cells up to passage 5 for 81 days. Furthermore, endometrial epithelium and stroma, after the freeze-thaw procedure and sequential culture, were able to establish an endometrial three-dimensional model. Conclusions We herein established a model of in vitro cultured endometrium as a potential therapeutic option for refractory thin endometrium. The three-dimensional culture model with endometrial epithelial and stromal cell orchestration via cytokines, membrane-bound molecules, extracellular matrices, and gap junction will provide a new framework for exploring the mechanisms underlying the phenomenon of implantation. Additionally, modified embryo culture, so-called “in vitro implantation”, will be possible therapeutic approaches in fertility treatment.

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