Can estimations of early stage embryo weight predict later embryo development rates?

2012 ◽  
Vol 98 (3) ◽  
pp. S270
Author(s):  
S.D. Prien ◽  
C. Wessels ◽  
L. Penrose
Keyword(s):  
Embryo Development ◽  
Early Stage ◽  
Development Rates ◽  
Stage Embryo ◽  
Early Stage Embryo

scholarly journals Fast Multi-Focus Fusion Based on Deep Learning for Early-Stage Embryo Image Enhancement

Sensors ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 863
Author(s):  
Vidas Raudonis ◽  
Agne Paulauskaite-Taraseviciene ◽  
Kristina Sutiene
Keyword(s):  
Deep Learning ◽  
Image Fusion ◽  
Early Stage ◽  
Image Data ◽  
Cell Detection ◽  
Processing Times ◽  
Fused Image ◽  
Stage Embryo ◽  
Early Stage Embryo

Background: Cell detection and counting is of essential importance in evaluating the quality of early-stage embryo. Full automation of this process remains a challenging task due to different cell size, shape, the presence of incomplete cell boundaries, partially or fully overlapping cells. Moreover, the algorithm to be developed should process a large number of image data of different quality in a reasonable amount of time. Methods: Multi-focus image fusion approach based on deep learning U-Net architecture is proposed in the paper, which allows reducing the amount of data up to 7 times without losing spectral information required for embryo enhancement in the microscopic image. Results: The experiment includes the visual and quantitative analysis by estimating the image similarity metrics and processing times, which is compared to the results achieved by two wellknown techniques—Inverse Laplacian Pyramid Transform and Enhanced Correlation Coefficient Maximization. Conclusion: Comparatively, the image fusion time is substantially improved for different image resolutions, whilst ensuring the high quality of the fused image.

scholarly journals NMR spectroscopy of a single mammalian early stage embryo

2021 ◽  
pp. 107142
Author(s):  
Giulia Sivelli ◽  
Gaurasundar M. Conley ◽  
Carolina Herrera ◽  
Kathryn Marable ◽  
Kyle J. Rodriguez ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Early Stage ◽  
Stage Embryo ◽  
Early Stage Embryo

scholarly journals Anticentromere antibody induced by immunization with centromere protein and Freund’s complete adjuvant may interfere with mouse early-stage embryo

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hanyan Liu ◽  
Yufen Zhang ◽  
Haiying Liu ◽  
Qing Huang ◽  
Ying Ying
Keyword(s):  
Early Stage ◽  
Protein A ◽  
Immunofluorescence Assay ◽  
Immunofluorescence Test ◽  
Anticentromere Antibody ◽  
Indirect Immunofluorescence Test ◽  
Centromere Protein ◽  
Centromere Protein A ◽  
Stage Embryo ◽  
Early Stage Embryo

Abstract Background Anticentromere antibody (ACA) is a member of the antinuclear antibody spectrum (ANAs) which has been speculated to be associated with subfertility. Thus, the present study aimed to investigate the induction of ACA production and its potential interference with early-stage embryos. Methods Recombinant centromere protein-A (CENP-A) or centromere protein-B (CENP-B) and complete Freund’s adjuvant (CFA) were used to immunize mice. Serum ACA level was then evaluated by using an indirect immunofluorescence test. Immunofluorescence assay was performed to detect IgG in follicles in ovarian tissues and early-stage embryos. Results Following treatment, serum positive ACA was observed in mice treated with CENP and CFA. Furthermore, IgG were detected in follicular fluid and early-stage embryos from mice treated with CENP and CFA. Conclusions This study preliminarily indicated that ACA induced by CENP and CFA may penetrate into the living embryos of early-stage in mice.

scholarly journals The effect of respiratory gases and incubation temperature on early stage embryonic development in sea turtles

2020 ◽  
Author(s):  
David Terrington Booth ◽  
Alexander Archibald-Binge ◽  
Colin James Limpus
Keyword(s):  
Carbon Dioxide ◽  
Embryonic Development ◽  
Embryo Development ◽  
Incubation Temperature ◽  
Early Stage ◽  
Sea Turtle ◽  
Low Oxygen ◽  
Prolonged Incubation ◽  
Mature Embryos ◽  
Stage Embryo

AbstractSea turtle embryos at high density nesting beaches experience relative high rates of early stage embryo death. One hypothesis to explain this high dead rate is that there is an increased probability that newly constructed nests are located close to maturing clutches whose metabolising embryos cause low oxygen levels, high carbon dioxide levels, and high temperatures. Although these altered environmental conditions are well tolerated by mature embryos, early stage embryos may not be as tolerant leading to an increase in their mortality. To test this hypothesis, we incubated newly laid sea turtle eggs for a week over a range of temperatures in different combinations of oxygen and carbon dioxide concentrations and assessed embryo development and death rates. We found that gas mixtures of decreased oxygen and increased carbon dioxide, similar to those found in natural sea turtle nest containing mature embryos, slowed embryonic development but did not influence embryo mortality of early stage embryos. In contrast, high incubation temperature not only decreased embryo development rate, but prolonged incubation at 34°C was fatal.

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