scholarly journals Small molecule tolfenamic acid and dietary spice curcumin treatment enhances antiproliferative effect in pancreatic cancer cells via suppressing Sp1, disrupting NF-kB translocation to nucleus and cell cycle phase distribution

2016 ◽  
Vol 31 ◽  
pp. 77-87 ◽  
Author(s):  
Riyaz Basha ◽  
Sarah F. Connelly ◽  
Umesh T. Sankpal ◽  
Ganji Purnachandra Nagaraju ◽  
Hassaan Patel ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Small Molecule ◽  
Phase Distribution ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Curcumin Treatment ◽  
Pancreatic Cancer Cells ◽  
Cell Cycle Phase Distribution

scholarly journals Anticancer and apoptotic effects of theaflavin-3-gallate in non-small cell lung carcinoma

2015 ◽  
Vol 10 (4) ◽  
pp. 790
Author(s):  
Da-Wei Li ◽  
Long Meng ◽  
Kui-Xing Zhang ◽  
Wei-Ke Zhang
Keyword(s):  
Cell Cycle ◽  
Cancer Cells ◽  
Phase Distribution ◽  
Small Cell ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Small Cell Lung ◽  
M Phase ◽  
Cell Cycle Phase Distribution ◽  
Apoptotic Effects

<p class="Abstract">The objective was to determine the antiproliferative and apoptotic effects of theaflavin-3-gallate in human non-small cell lung cancer cells (A-549) along with determining the effect on cell cycle phase distribution, cell migration and invasion. Cell viability was determined by MTT assay while as phase contrast and fluorescence microscopies were involved to study apoptotic morphologi-cal features in these cells. Flow cytometry investigated the effect of theaflavin-3-gallate on cell cycle phase distribution. Theaflavin-3-gallate treatment led to a substantial cytotoxic effect in A-549 cancer cells with IC<sub>50</sub> values of 42.1 µM and 27.9 µM at 24 and 48 hours respectively. Further, 80 and 160 µM dose of theaflavin-3-gallate induced apoptotic features including chromatin margina-tion and micronuclei presence. The population of cells in G2/M phase increased from 2.7% (control) to 6.8%, 17.2% and finally to 46.5% after treatment with 20, 80 and 160 µM concentration of theaflavin-3-gallate respectively indicating G2/M phase cell cycle arrest.</p><p> </p>

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