Effect of Mineral Trioxide Aggregate on Rat Clonal Dental Pulp Cells: Expression of Cyclooxygenase-2 mRNA and Inflammation-related Protein via Nuclear Factor Kappa B Signaling System

2009 ◽  
Vol 35 (6) ◽  
pp. 843-846 ◽  
Author(s):  
Hajime Minamikawa ◽  
Yoshiaki Deyama ◽  
Kouichi Nakamura ◽  
Yoshitaka Yoshimura ◽  
Masayuki Kaga ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Nuclear Factor Kappa B ◽  
Mineral Trioxide Aggregate ◽  
Cyclooxygenase 2 ◽  
Nuclear Factor ◽  
Dental Pulp Cells ◽  
Related Protein ◽  
Signaling System ◽  
Nuclear Factor Kappa ◽  
Pulp Cells

scholarly journals Differential Gene Expression Changes in Human Primary Dental Pulp Cells Treated with Biodentine and TheraCal LC Compared to MTA

Biomedicines ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 445
Author(s):  
Ok Hyung Nam ◽  
Ho Sun Lee ◽  
Jae-Hwan Kim ◽  
Yong Kwon Chae ◽  
Seoung-Jin Hong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Viability ◽  
Differential Gene Expression ◽  
Dental Pulp ◽  
Nuclear Factor ◽  
Dental Pulp Cells ◽  
Nuclear Factor Kappa ◽  
Gene Sets ◽  
Pulp Cells ◽  
Differential Gene

This study aimed to analyze the effects of pulp capping materials on gene expression changes in primary tooth-derived dental pulp cells using next-generation sequencing. Dental pulp cells were extracted and treated with mineral trioxide aggregate (MTA), Biodentine (BD), or TheraCal LC (TC). Cell viability assays were performed. Total RNA was extracted and analyzed through mRNA sequencing. Bioinformatic analysis of differential gene expression in dental pulp cells exposed to BD or TC versus MTA was performed. MTA, BD, and TC exposure had no significant effect on pulp cell viability (p > 0.05). Gene sets associated with inflammatory response (p = 2.94 × 10−5) and tumor necrosis factor alpha (TNF-α) signaling via the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway (p = 2.94 × 10−5) were enriched in all materials. In BD-treated cells, Wnt/β-catenin signaling (p = 3.15 × 10−4) gene sets were enriched, whereas enrichment of interferon gamma (IFN-γ) response (p = 3 × 10−3) was observed in TC-treated cells. In gene plot analysis, marked increases in receptor activator of nuclear factor kappa-Β ligand (RANKL) expression were seen in TC-treated cells over time. Despite the similar cell viabilities exhibited among MTA-, BD-, and TC-treated cells, patterns of gene networks differed, suggesting that diverse functional gene differences may be associated with treatment using these materials.

Effect of Nifedipine on the Differentiation of Human Dental Pulp Cells Cultured with Mineral Trioxide Aggregate

2013 ◽  
Vol 39 (6) ◽  
pp. 801-805 ◽  
Author(s):  
Su-Mi Woo ◽  
Yun-Chan Hwang ◽  
Hoi-Soon Lim ◽  
Nam-Ki Choi ◽  
Sun-Hun Kim ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Mineral Trioxide Aggregate ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Cells Cultured

The Effect of Mineral Trioxide Aggregate on the Mineralization Ability of Rat Dental Pulp Cells: An In Vitro Study

2008 ◽  
Vol 34 (9) ◽  
pp. 1057-1060 ◽  
Author(s):  
Yoshiyuki Yasuda ◽  
Masafumi Ogawa ◽  
Toshiya Arakawa ◽  
Tomoko Kadowaki ◽  
Takashi Saito
Keyword(s):  
Dental Pulp ◽  
Mineral Trioxide Aggregate ◽  
In Vitro Study ◽  
Vitro Study ◽  
Dental Pulp Cells ◽  
Pulp Cells

Mineral Trioxide Aggregate with Mussel-inspired Surface Nanolayers for Stimulating Odontogenic Differentiation of Dental Pulp Cells

2018 ◽  
Vol 44 (6) ◽  
pp. 963-970 ◽  
Author(s):  
Ming-Gene Tu ◽  
Chia-Che Ho ◽  
Tuan-Ti Hsu ◽  
Tsui-Hsien Huang ◽  
Min-Jhen Lin ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Mineral Trioxide Aggregate ◽  
Dental Pulp Cells ◽  
Odontogenic Differentiation ◽  
Pulp Cells
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