In Vitro Osteogenic Potential of an Experimental Calcium Silicate–based Root Canal Sealer

ABSTRACTIntroduction: The aim of this in vitro study was to assess the pH values of selected calcium-silicate root canal sealers in comparison with AH Plus resin-based root canal sealer.Materials and methods: Six root canal sealers were included in this study: BioRoot RCS (Septodont, France), GuttaFlow bioseal (Coltėne/Whaledent, Switzerland), MTA Fillapex (Angelus, Brazil), Total Fill BC Sealer (FKG, Switzerland), Well-Root ST (Vericom, Korea) and AH Plus (Dentsply, DeTrey, Germany). Materials samples (n = 6) were placed in dishes and immersed in 10 mL of distilled water (pH = 6.8). After 1, 3, 24, 48, 72, 168, 336 and 504 hrs, water pH was determined with a pH meter calibrated with a solution of known pH. Data were statistically analyzed using Student-t test (p < 0.05).Results: There were differences in the water pH between the materials tested. The highest water pHs were exhibited by Total Fill BC Sealer, Well-Root ST and BioRoot RCS (pH ~12), followed by GuttaFlow bioseal (pH ~10.5) and MTA Fillapex (pH ~9). The lowest pH was shown by AH Plus (pH ~8).Conclusions: All calcium-silicate root canal sealers studied are able to elevate the pH level, which could have biological and antimicrobial effects. The AH Plus root canal sealer has poor alkalizing properties.

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In vitro cytocompatibility and osteogenic potential of calcium silicate-based dental cements in a root canal-filling model

Journal of International Medical Research ◽

10.1177/0300060519894801 ◽

2019 ◽

Vol 48 (4) ◽

pp. 030006051989480

Author(s):

Yan Jing ◽

Ting Gong ◽

Chenggang Duan ◽

Huan Wang ◽

Chengfei Zhang ◽

...

Keyword(s):

Root Canal ◽

Calcium Silicate ◽

Negative Control ◽

Osteogenic Potential ◽

Filling Material ◽

Periodontal Ligament Stem Cells ◽

Root Canal Filling ◽

Root Cells ◽

Ah Plus

Objective To investigate the in vitro cytocompatibility and osteogenic potential of an experimental calcium silicate-based cement and the inflammatory response in human periodontal ligament stem cells (hPDLSCs). Methods Cellular responses, osteogenic-related gene expression, and the production of inflammatory cytokines including interleukin (IL)-6 and IL-8 were studied in hPDLSCs exposed to the experimental root canal-filling material C-Root, the commercial tricalcium silicate-based material BioRoot RCS, and the epoxy resin-based material AH Plus. Differences were analyzed using one-way ANOVA with Bonferroni-adjusted pairwise comparison. Results Exposure to BioRoot and C-Root caused time-dependent increases in cell proliferation. Significantly more mineralized nodules were formed in cells exposed to AH Plus and BioRoot compared with the negative control. Alkaline phosphatase (ALP) activity was significantly lower in AH Plus cells compared with negative control, BioRoot, and C-Root cells. ALP, osteocalcin (OCN), and runt-related transcription factor2 (RUNX2) mRNA expression levels were all significantly higher in C-Root compared with AH Plus cells at day 7. IL-6 and IL-8 levels differed significantly among the experimental groups, with the highest IL-8 levels in BioRoot cells at days 7 and 14. Conclusion The experimental root canal-filling material C-Root has similar in vitro cytocompatibility to BioRoot and better osteogenic potential than AH Plus.

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In Vitro Evaluation of the Sealing Ability of Newly Developed Calcium Phosphate–based Root Canal Sealer

Journal of Endodontics ◽

10.1016/j.joen.2006.07.023 ◽

2007 ◽

Vol 33 (8) ◽

pp. 978-981 ◽

Cited By ~ 14

Author(s):

Sung-Eun Yang ◽

Seung-Ho Baek ◽

WooCheol Lee ◽

Kee-Yeon Kum ◽

Kwang-Shik Bae

Keyword(s):

Calcium Phosphate ◽

Root Canal ◽

Root Canal Sealer ◽

In Vitro Evaluation ◽

Sealing Ability

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Comparative evaluation of retreatability of calcium silicate-based root canal sealers and epoxy resin-based root canal sealers in curved canals-An In-Vitro micro-CT analysis

Indian Journal of Dental Research ◽

10.4103/ijdr.ijdr_328_20 ◽

2021 ◽

Vol 32 (1) ◽

pp. 79

Author(s):

MV Mavishna ◽

KondasVijay Venkatesh

Keyword(s):

Epoxy Resin ◽

Root Canal ◽

Calcium Silicate ◽

Comparative Evaluation ◽

Micro Ct ◽

Ct Analysis ◽

Root Canal Sealers

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Effectiveness of a silicon-based root canal sealer for filling of simulated lateral canals

Brazilian Dental Journal ◽

10.1590/s0103-64402007000100005 ◽

2007 ◽

Vol 18 (1) ◽

pp. 20-23 ◽

Cited By ~ 2

Author(s):

João Vicente Baroni Barbizam ◽

Matheus Souza ◽

Doglas Cecchin ◽

Jakob Dabbel

Keyword(s):

Root Canal ◽

In Vitro Study ◽

Root Canal Sealer ◽

Human Teeth ◽

Significance Level ◽

Working Length ◽

Group 3 ◽

Group 2 ◽

Silicon Based

The purpose of this in vitro study was to evaluate the ability of a silicon-based root canal sealer, compared to zinc oxide and eugenol and an epoxy resin-based sealers, for filling of simulated lateral canals. Thirty extracted single-rooted human teeth were selected, conventional access was made and the working length was established 1 mm from the apical foramen. Three simulated lateral canals, one in each root third (coronal, middle and apical) were prepared in both the mesial and distal surfaces of each tooth using a size 15 reamer adapted to a low-speed handpiece. Each root canal was instrumented using ProTaper rotary files up to file F3 at the working length, and then irrigated with 2.5% NaOCl followed by EDTA. The teeth were assigned to 3 groups (n=10), according to the root canal sealer: Roeko Seal (Group 1), Sealer 26 (group 2) and Grossman's sealer (Group 3). Gutta-percha cold lateral condensation technique was performed in all groups. Postoperative radiographs were taken and the images were projected for evaluation of the quality of lateral canal filling. Data were submitted to statistical analysis by Kruskal Wallis test at 5% significance level. The results showed that Grossman's sealer filled a larger number of lateral canals than Roeko Seal (p<0.05) and Sealer 26 (p<0.01). It may be concluded that Roeko Seal silicone-based root canal sealer was not as effective as the Grossman's sealer for filling of simulated lateral canals. The lateral canals localized in the apical third of the root were more difficult to be filled.

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