Salivary Mediated Autoinoculation of Herpes Simplex Virus on the Face in the Absence of “Cold Sores,” After Trauma

The clinical presentations of herpes simplex virus (HSV) infections are varied and range from asymptomatic to a prodrome of tingling and burning followed by painful vesicles, erosions and ulcers. Resolution leads to latent infection of the sensory ganglia. HSV-1 is associated with most of the nongenital HSV–induced infections and HSV-2 is generally associated with anogenital lesions; however, lesions at either site may be caused by both viruses. In persons living with HIV (PLHIV), the lesions have been described as verrucous/hypertrophic, exophytic or vegetative and may suggest a neoplastic rather than an infective process and this can be a diagnostic dilemma in resource-limited countries with no access to confirmatory diagnostic testing for HSV. We report on two PLHIV who developed rapidly growing lesions on the face that clinically mimicked neoplasia but were found to be HSV-associated squamous proliferative lesions which responded to high dose acyclovir.

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Alzheimer's disease, herpes simplex virus type 1, cold sores and apolipoprotein E4

Biochemical Society Transactions ◽

10.1042/bst023594s ◽

1995 ◽

Vol 23 (4) ◽

pp. 594S-594S ◽

Cited By ~ 19

Author(s):

WOAN-RU LIN ◽

DAZHUANG SHANG ◽

G.K. WILCOCK ◽

RUTH F. ITZHAKI

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Herpes Simplex Virus ◽

Herpes Simplex ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Apolipoprotein E4 ◽

Cold Sores ◽

Simplex Virus

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The Herpes Simplex Virus 1 UL51 Protein Interacts with the UL7 Protein and Plays a Role in Its Recruitment into the Virion

Journal of Virology ◽

10.1128/jvi.02799-14 ◽

2014 ◽

Vol 89 (6) ◽

pp. 3112-3122 ◽

Cited By ~ 27

Author(s):

Richard J. Roller ◽

Rachel Fetters

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Virus Assembly ◽

Viral Glycoprotein ◽

Herpes Simplex Virus 1 ◽

Independent Functions ◽

The Face ◽

Infected Cells ◽

Simplex Virus ◽

Cell Spread

ABSTRACTThe alphaherpesvirus UL51 protein is a tegument component that interacts with the viral glycoprotein E and functions at multiple steps in virus assembly and spread in epithelial cells. We show here that pUL51 forms a complex in infected cells with another conserved tegument protein, pUL7. This complex can form in the absence of other viral proteins and is largely responsible for recruitment of pUL7 to cytoplasmic membranes and into the virion tegument. Incomplete colocalization of pUL51 and pUL7 in infected cells, however, suggests that a significant fraction of the population of each protein is not complexed with the other and that they may accomplish independent functions.IMPORTANCEThe ability of herpesviruses to spread from cell to cell in the face of an immune response is critical for disease and shedding following reactivation from latency. Cell-to-cell spread is a conserved ability of herpesviruses, and the identification of conserved viral genes that mediate this process will aid in the design of attenuated vaccines and of novel therapeutics. The conserved UL51 gene of herpes simplex virus 1 plays important roles in cell-to-cell spread and in virus assembly in the cytoplasm, both of which likely depend on specific interactions with other viral and cellular proteins. Here we identify one of those interactions with the product of another conserved herpesvirus gene, UL7, and show that formation of this complex mediates recruitment of UL7 to membranes and to the virion.

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Multiple recurrences of HSV-1 on right lower buttock

The Southwest Respiratory and Critical Care Chronicles ◽

10.12746/swrccc.v7i27.492 ◽

2019 ◽

Vol 7 (27) ◽

pp. 72-74

Author(s):

Surav Man Sakya ◽

Judy Park DeWitt ◽

Ashley Sturgeon

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

General Population ◽

Herpes Simplex Virus Type ◽

Anogenital Region ◽

The Face ◽

Simplex Virus ◽

Multiple Recurrences ◽

Hsv 1

Herpes simplex virus type 1 (HSV-1) is a common localized viral infection that affects many individuals in the general population. HSV-1 infection most commonly presents on the face and lips. In contrast, HSV-2 most commonly arises clinically in a boxer distribution on the anogenital region, which can include the buttocks. We report a healthy 28-year-old Asian female patient with multiple reoccurrences of grouped vesicles with an erythematous base on her right inferior medial buttock. On viral blood testing, the etiological agent was HSV-1, not HSV-2. The novelty of this case is the manifestation of HSV-1, not HSV-2 on this location.

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Assembly of VP26 in herpes simplex virus-1 capsid implicated by 3-D structure of recombinant capsid

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100140579 ◽

1995 ◽

Vol 53 ◽

pp. 840-841

Author(s):

Z. Hong Zhou ◽

Jing He ◽

Joanita Jakana ◽

J. D. Tatman ◽

Frazer J. Rixon ◽

...

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

3D Structure ◽

Structure Study ◽

Herpes Simplex Virus 1 ◽

Shell Proteins ◽

Life Threatening ◽

Infected Cells ◽

Simplex Virus ◽

Hsv 1

Herpes simplex virus-1 (HSV-1) is a ubiquitous virus which is implicated in diseases ranging from self-curing cold sores to life-threatening infections. The 2500 Å diameter herpes virion is composed of a glycoprotein spike containing, lipid envelope, enclosing a protein layer (the tegument) in which is embedded the capsid (which contains the dsDNA genome). The B-, and A- and C-capsids, representing different morphogenetic stages in HSV-1 infected cells, are composed of 7, and 5 structural proteins respectively. The three capsid types are organized in similar T=16 icosahedral shells with 12 pentons, 150 hexons, and 320 connecting triplexes. Our previous 3D structure study at 26 Å revealed domain features of all these structural components and suggested probable locations for the outer shell proteins, VP5, VP26, VP19c and VP23. VP5 makes up most of both pentons and hexons. VP26 appeared to bind to the VP5 subunit in hexon but not to that in penton.

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Application of EM to differentiate herpes virus from pox virus in genital specimens

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100169043 ◽

1994 ◽

Vol 52 ◽

pp. 262-263

Author(s):

K. Rekrut ◽

K. Schleuter

Keyword(s):

Tissue Culture ◽

Herpes Simplex Virus ◽

Herpes Simplex ◽

Fresh Tissue ◽

Culture Cells ◽

Viral Transport ◽

Carbon Coated ◽

Immuno Assay ◽

Simplex Virus ◽

Enzyme Immuno Assay

Confirmation of herpes simplex virus (HSV) from genital lesions of obstetrical (OB) patients may affect both the management of the delivery and of the neonate.(l,2) During 1992 and 1993, 4,450 genital specimens from OB patients were submitted in viral transport media for herpes culture. The specimens were inoculated into MRC-5, Vero, and A-549 tissue culture tubes, incubated, and examined daily for 7 days for cytopathic effect (CPE). The original specimens were frozen at −70° C until final reports were issued. Culture tubes with CPE were tested by the Dupont Herpchek enzyme immuno assay (EIA) to confirm the presence of herpes simplex virus (HSV). (3,4) 170 OB patient specimens were positive by culture and confirmed by EIA.There were also 63 cultures exhibiting CPE ressembling HSV which were negative by EIA testing, which failed to pass in fresh tissue culture cells or progress to more enhanced CPE in culture. These original specimens were screened by electron microscopy after direct ultracentrifugation employing the Beckman airfuge with the EM 90 rotor on to formvar carbon-coated 300 mesh copper grids and negatively stained with 2% PTA.

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